The Selection and Validation of Reference Genes for RT-qPCR Analysis of the Predatory Natural Enemy (Hemiptera: Anthocoridae).

is a highly effective natural enemy for controlling thrips, tetranychids, aphids, and various Lepidoptera pests. Nevertheless, the molecular mechanisms underlying its interactions with host pests remain unclear. Screening for optimal reference genes is a prerequisite for using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to investigate the interrelationship.

Insulin Signaling Pathway Mediates FoxO-Pepck Axis Regulation of Glucose Homeostasis in .

The agricultural pest exhibits a strong preference for feeding on fresh fruits, demonstrating high adaptability to sugary environments. Meanwhile, high sugar levels stimulate insulin secretion, thereby regulating the steady state of sugar metabolism. Understanding the mechanisms related to sugar metabolism in is crucial due to its adaptation to these specific environmental conditions.

Bioaccumulation and transferreing for impacts on Cd and Pb by aphid consumption of the broad bean, Vicia faba L, in soil heavy metal pollution.

Heavy metal pollution threatens human and ecological health. Heavy metals can exist in the soil for a long time and migrate to organisms along the food chain. However, only a few studies have investigated the effects of a single stress on broad beans.

Molecular Correlates of Diapause in .

The aphidophagous gall midge, (Rondani) (Diptera: Cecidomyiidae), a dominant natural enemy of aphids, has been used as a biological control agent in many countries to control aphids in greenhouses. To identify key factors that induce diapause in , we evaluated the effects of photoperiod and temperature on the incidence of diapause in under laboratory conditions. The results showed that temperature and photoperiod had significant impacts on development and diapause in Low temperatures and a short photoperiod inhibited development, while high temperatures and a long photoperiod promoted development.

Control effect and field application of four predatory Orius species on Megalurothrips usitatus (Thysanoptera: Thripidae).

Megalurothrips usitatus (Bagrall) is one of the most important pests of cowpea, Vigna unguiculata (Linn.) Walp in South China. Four Orius species, including Orius minutus (L.

Effect of Guanylate Cyclase-22-like on Ovarian Development of (Hemiptera: Anthocoridae).

This study identified and characterized the gene encoding recep tor-type guanylate cyclase-22-like (GCY-22; ) from the pirate bug , an important biological control agent. The full-length cDNA of the of was obtained by rapid amplification of cDNA ends (RACE); it had a total length of 4888 base pairs (bp), of which the open reading frame (ORF) was 3750 bp, encoding a polypeptide of 1249 amino acid residues. The physicochemical properties of were predicted and analyzed by using relevant ExPASy software, revealing a molecular formula of C6502H10122N1698O1869S57, molecular weight of ~143,811.

Comparative 4D Label-Free Quantitative Proteomic Analysis of Provides Insights into Proteins and Processes Associated with Diapause.

Diapause, an adaptative strategy for survival under harsh conditions, is a dynamic multi-stage process. , an important agricultural pollinator, is declining in the wild, but artificial breeding is possible by imitating natural conditions. Mated queen bees enter reproductive diapause in winter and recover in spring, but the regulatory mechanisms remain unclear.

knockout affects development and fecundity of .

Forkhead box O (FoxO), a key transcription factor in many species, participates in numerous physiological and pathological processes of organisms through a variety of signaling pathways. In the present study, we established knockout (-KO) strain using CRISPR/Cas9, and the influence on development and fecundity of mutant strain were evaluated. To clarify the corresponding mechanism, a transcriptome analysis was conducted subsequently.

Exogenous Calcium Suppresses the Oviposition Choices of (Thysanoptera: Thripidae) and Promotes the Attraction of (Hemiptera: Anthocoridae) by Altering Volatile Blend Emissions in Kidney Bean Plants.

is a destructive pest of horticultural plants, while is a natural enemy of thrips. It has been demonstrated that exogenous calcium could induce plant defenses against herbivore attack. We examined whether CaCl supplementation altered the volatile emissions of kidney bean plants, which influence the oviposition preference of .

Management through Combined Use of Beneficial Insects and Thiacloprid in Pepper Seedlings.

insecticide application has posed a threat to pollinators and has also increased insecticide resistance of Sulzer. Therefore, it is urgent to develop an economical and effective strategy, especially for greenhouse vegetables. Firstly, we selected a neonicotinoid insecticide that is specifically fatal to but relatively safe to predators and bumblebees by laboratory toxicity tests and risk assessments.

Quantitative Proteomic and Transcriptomic Analyses of Metabolic Regulation of Adult Reproductive Diapause in (Diptera: Drosophilidae) Females.

Diapause is a form of dormancy used by many insects to survive adverse environmental conditions, which can occur in specific developmental stages in different species. is a serious economic pest and we determined the conditions for adult reproductive diapause by the females in our previous studies. In this study, we combined RNA-Seq transcriptomic and quantitative proteomic analyses to identify adult reproductive diapause-related genes and proteins.

FoxO Transcription Factor Regulate Hormone Mediated Signaling on Nymphal Diapause.

Diapause is a complex physiological adaptation phenotype, and the transcription factor Forkhead-box O (FoxO) is a prime candidate for activating many of its diverse regulatory signaling pathways. Hormone signaling regulates nymphal diapause in . Here, the function of the gene isolated from was investigated.

Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata.

Chitin synthases are very important enzymes for chitin synthesis in various species, which makes them a specific target of insecticides. In the present study, the function of the chitin synthase A (CHSA) gene isolated from Mythimna separate is investigated. The majority of dsMysCHSA treated larvae (89.