Molecular probes for tracking lipid droplet membrane dynamics.

Lipid droplets (LDs) feature a unique monolayer lipid membrane that has not been extensively studied due to the lack of suitable molecular probes that are able to distinguish this membrane from the LD lipid core. In this work, we present a three-pronged molecular probe design strategy that combines lipophilicity-based organelle targeting with microenvironment-dependent activation and design an LD membrane labeling pro-probe called LDM. Upon activation by the HClO/ClO microenvironment that surrounds LDs, LDM pro-probe releases LDM-OH probe that binds to LD membrane proteins thus enabling visualization of the ring-like LD membrane.

visualization of the cellular uptake and sub-cellular distribution of mussel oligosaccharides.

Unlike chemosynthetic drugs designed for specific molecular and disease targets, active small-molecule natural products typically have a wide range of bioactivities and multiple targets, necessitating extensive screening and development. To address this issue, we propose a strategy for the direct microdynamic examination of potential drug candidates to rapidly identify their effects and mechanisms of action. As a proof-of-concept, we investigated the behavior of mussel oligosaccharide (MOS-1) by tracking the subcellular dynamics of fluorescently labeled MOS-1 in cultured cells.

Prediction model for asymptomatic carotid atherosclerosis using retinal microvascular intelligent analysis: A retrospective study.

Importance: Early detection and timely diagnosis of asymptomatic carotid atherosclerosis significantly assist in the prevention of ischemic stroke for them.

Objective: This observational study aimed to develop and validate a novel prediction model to assist in the early diagnosis of carotid atherosclerosis based on new characteristic variables screened by retinal microvascular intelligence analysis.

Main Outcome(s) And Method (s): The least absolute shrinkage and selection operator (LASSO) combined with 10-fold cross-validation were screened for characteristic variables, and nomograms were plotted to demonstrate the prediction model.

Corrigendum: Impact of a mobile health intervention based on multi-theory model of health behavior change on self-management in patients with differentiated thyroid cancer: protocol for a randomized controlled trial.

[This corrects the article DOI: 10.3389/fpubh.2024.

Selective production of methylindan and tetralin with xylose or hemicellulose.

Indan and tetralin are widely used as fuel additives and the intermediates in the manufacture of thermal-stable jet fuel, many chemicals, medicines, and shockproof agents for rubber industry. Herein, we disclose a two-step route to selectively produce 5-methyl-2,3-dihydro-1H-indene (abbreviated as methylindan) and tetralin with xylose or the hemicelluloses from agricultural or forestry waste. Firstly, cyclopentanone (CPO) was selectively formed with ~60% carbon yield by the direct hydrogenolysis of xylose or hemicelluloses on a non-noble bimetallic Cu-La/SBA-15 catalyst.

Chronic obstructive pulmonary disease as a risk factor for sarcopenia: A systematic review and meta-analysis.

Sarcopenia prevalence and its risk factors in chronic obstructive pulmonary disease (COPD) vary partly due to definition criteria. This systematic review aimed to identify the prevalence and risk factors of sarcopenia in COPD patients. This review was registered in PROSPERO (CRD42022310750).

The association between passive smoking and sleep quality in a Chinese hypertensive population: A cross-sectional study.

Introduction: This study evaluates the association between passive smoking, specifically secondhand smoke (SHS) and thirdhand smoke (THS) exposure, and sleep quality in a hypertensive population.

Methods: We enrolled 1427 eligible hypertensive patients from a 2022 national cross-sectional survey in China. Data on tobacco smoke exposure and sleep were collected via questionnaires.

Impact of a mobile health intervention based on multi-theory model of health behavior change on self-management in patients with differentiated thyroid cancer: protocol for a randomized controlled trial.

Introduction: Theoretical models of health behavior are important guides for disease prevention and detection, treatment and rehabilitation, and promotion and maintenance of physical and mental health, but there are no intervention studies related to differentiated thyroid cancer (DTC) that use theoretical models of health as a guide. In this study, we used a microblogging platform as an intervention vehicle and mobile patient-doctor interactive health education as a means of intervention, with the aim of improving the health behaviors of DTC patients as well as the corresponding clinical outcomes.

Methods: This research project is a quantitative methodological study, and the trial will be a single-blind, single-center randomized controlled trial conducted at the Fourth Hospital of Harbin Medical University in Harbin, Heilongjiang Province.

Individualised monitoring programme for pulmonary rehabilitation of patients with chronic obstructive pulmonary disease-study protocol for a randomised controlled trial.

Background: Chronic obstructive pulmonary disease (COPD) is characterised mainly by exertional dyspnoea, which may lead to activity reduction. Pulmonary rehabilitation (PR) is considered capable of mitigating these impairments. However, access to PR is limited to specialised centres, especially during the coronavirus disease 2019 pandemic.

Relationship Between Physical Exercise and Cognitive Impairment Among Older Adults with Type 2 Diabetes: Chain Mediating Roles of Sleep Quality and Depression.

Objective: Although physical exercise has been shown to boost physical, psychological, and psychiatric conditions in older adults, there is a relative lack of research on the mechanisms involved in this process for older adults with type 2 diabetes mellitus (T2DM). We thus evaluated whether sleep quality and depression mediated the relationship between physical exercise and cognitive impairment in older adults with T2DM by focusing on the exercise-physiology-psychology and psychiatry connection.

Methods: Self-reported data were collected from 2646 older adults with T2DM in Weifang, Shandong, China.

Synthesis of Cyclopentadiene and Methylcyclopentadiene with Xylose or Extracted Hemicellulose.

Cyclopentadiene (CPD) and methylcyclopentadiene (MCPD) are important intermediates that have been widely used in the production of high-energy-density rocket fuels, polymers and valuable chemicals. Currently, CPD and MCPD are produced from fossil energies at very low yields, which greatly limits their application. As a solution to this problem, we disclose an alternative two-step bio-route to access CPD and MCPD using xylose or extracted hemicellulose as the feedstock.

Fluorescent Probes Design Strategies for Imaging Mitochondria and Lysosomes.

Modern cellular biology faces several major obstacles, such as the determination of the concentration of active sites corresponding to chemical substances. In recent years, the popular small-molecule fluorescent probes have completely changed the understanding of cellular biology through their high sensitivity toward specific substances in various organisms. Mitochondria and lysosomes are significant organelles in various organisms, and their interaction is closely related to the development of various diseases.

Research on the Operating Mechanism of E-Commerce Poverty Alleviation in Agricultural Cooperatives: An Actor Network Theory Perspective.

E-commerce poverty alleviation has become a new wisdom in China's rural poverty alleviation, but there are a few empirical researches on e-commerce poverty alleviation based on farmer cooperatives. Taking four typical poverty counties in Zhejiang Province as an example, based on the actor network theory (ANT), this paper defines the participants and their obligatory passage point (OPP) from the e-commerce poverty alleviation actor network (EPAAN), combs the roles and interest demands of various stakeholders, and constructs the EPAAN model based on farmer cooperatives according to the translation process. We found that the EPAAN is a heterogeneous network by constantly updating, which consists of human actors with cooperatives as the main body and non-human actors.

Functional Connectivity Underlying Symptoms in Preschool Boys With Autism: A Resting-State Functional Magnetic Resonance Imaging Study.

Background: Single-sex children have been regarded as one of the best subjects to understand the abnormal development patterns of autism spectrum disorders (ASDs). However, the functional connectivity (FC) behind their symptoms is still unknown.

Methods: Based on FC analysis, the acquired resting-state functional magnetic resonance imaging (rs-fMRI) data sets, including 86 boys with ASD and 54 normal controls (NC), were used to detect the neural synchronous activity between brain regions.

One-Step Acidic Hydrothermal Preparation of Dendritic Rutile TiO₂ Nanorods for Photocatalytic Performance.

Three-dimensional and dendritic rutile TiO₂ nanorods were successfully fabricated on a Ti foil surface using a one-step acidic hydrothermal method. The TiO₂ nanorods were characterized using X-ray diffraction (XRD), energy dispersive X-ray spectrometry (EDX), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and optical contact angle testing. The results showed that the nanorods with diameters of 100⁻500 nm and lengths of 100 nm to 1 μm were obtained on the Ti foil surface.

Contracting with private providers for primary care services: evidence from urban China.

Controversy surrounds the role of the private sector in health service delivery, including primary care and population health services. China's recent health reforms call for non-discrimination against private providers and emphasize strengthening primary care, but formal contracting-out initiatives remain few, and the associated empirical evidence is very limited. This paper presents a case study of contracting with private providers for urban primary and preventive health services in Shandong Province, China.