Diverse RNA methylation patterns in neutrophils: key drivers in hepatocellular carcinoma.

Background: Neutrophils, crucial in the immune system, have recently been implicated in promoting malignancy. RNA methylation, an essential epigenetic feature, plays a key role in tumor microenvironment (TME) reprogramming. However, the relationship between neutrophils and RNA methylation in hepatocellular carcinoma (HCC) remains unclear.

Assessing and Comparing Potential Allergenicity of Two Partially Hydrolyzed Whey-Based Formulas for Infants: A Population-Based Study in China.

Scope: In cases where breast milk is unavailable or inadequate, hydrolyzed infant formula is recommended as the primary alternative. The aim of this study is to assess and compare the allergenicity of two partially hydrolyzed whey-based formulas (PHF-Ws) using serum samples from patients with cow's milk allergy (CMA).

Methods And Results: LC-MS/MS technology is used to investigate the peptide distribution in both samples.

Infection evaluation in the early period after liver transplantation: A single-center exploration.

Background: Infection is a significant risk factor that impacts for perioperative morbidity and mortality in liver transplantation (LTx) patients and is difficult to evaluate quantitatively in the early posttransplantation period. Thus, a biomarker to assess the risk of infection and the prognosis of the recipient is highly desirable.

Methods: A total of 128 consecutive patients with end-stage liver diseases undergoing LTx between January 1, 2020 and December 31, 2021, at the First Affiliated Hospital of Zhejiang University School of Medicine, were screened retrospectively.

Evaluating the Response and Safety of Inactivated COVID-19 Vaccines in Liver Transplant Recipients.

Purpose: To evaluate the response and safety of an inactivated vaccine (Sinovac Life Sciences Co., Ltd., Beijing, China) for coronavirus disease 2019 (COVID-19) in liver transplant (LTx) recipients from China.

Sarcomatoid hepatocellular carcinoma: From clinical features to cancer genome.

Background: Sarcomatoid hepatocellular carcinoma (HCC) is a rare and highly lethal histological subtype of HCC, with completely unknown genetic etiology and therapeutic targets.

Methods: We included 16 patients with sarcomatoid HCC receiving radical resection among 6731 cases of pathological confirmed HCC in year 2008 to 2018 in our hospital. We compared the clinical features, prognosis and cancer genome between 15 sarcomatoid HCC and propensity score-matched 75 non-sarcomatoid HCC patients.

Aberrantly DNA Methylated-Differentially Expressed Genes and Pathways in Hepatocellular Carcinoma.

Methylation plays a significant role in the etiology and pathogenesis of hepatocellular carcinoma (HCC). The aim of the present study is to identify aberrantly methylated-diferentially expressed genes (DEGs) and dysregulated pathways associated with the development of HCC through integrated analysis of gene expression and methylation microarray. Aberrantly methylated-DEGs were identified from gene expression microarrays (GSE62232, GSE74656) and gene methylation microarrays (GSE44909, GSE57958).

Fatty acids promote fatty liver disease via the dysregulation of 3-mercaptopyruvate sulfurtransferase/hydrogen sulfide pathway.

Objective: Accumulation of free fatty acids (FFAs) in hepatocytes induces lipotoxicity, leading to non-alcoholic fatty liver disease (NAFLD). This study aimed to investigate the underlying mechanisms by which FFA contributes to the pathogenesis of NAFLD via the regulation of 3-mercaptopyruvate sulfurtransferase (MPST), a key enzyme that regulates endogenous hydrogen sulfide (HS) biosynthesis.

Design: Hepatic MPST expression was evaluated in mice and patients with NAFLD.

Liver transplantation for hepatocellular carcinoma beyond the Milan criteria.

Objective: Liver transplantation is an optimal radical therapy for selected patients with hepatocellular carcinoma. The stringent organ allocation system driven by the Milan criteria has been challenged by alternative sets of expanded criteria. Careful analysis is needed to prove that the Milan criteria can be expanded safely and effectively.

Liver transplantation in Crigler-Najjar syndrome type I disease.

Background: Crigler-Najjar syndrome type I (CNS I) is a very rare autosomal recessive inherited disease that liver transplantation can properly deal with.

Methods: We present one case of an 18-month-old child with CNS I diagnosed by clinical findings and genetic detecting. LTx was performed 5 days after kernicterus broke out and neurological symptoms were successfully reversed.

A novel model for evaluating the risk of hepatitis B recurrence after liver transplantation.

Background: Hepatitis B virus (HBV) recurrence is a major risk factor affecting the long-term survival of recipients for liver transplantation (LTx). Therefore, a model that can assess this risk before transplantation is highly desirable.

Methods: One hundred and eighty-five consecutive liver transplant recipients because of HBV-related end-stage liver diseases were selected.

[Progress in quality analysis of honey by infrared spectroscopy].

The detection of the quality of honey and the differentiation of adulteration are very important for quality and safety assurance. Traditionally used chemical methods were expensive and complicated, therefore they are not suitable for the requirement of wide-scale detection. In the past decade, the detection technology of honey developed with a trend of fast and high throughput detection.

[Possibilities of near-infrared spectroscopy for the assessment of principle components in honey].

The potential of near infrared spectroscopy (NIR) as a nondestructive method for determining the principle components of honeys was studied for 153 unifloral honeys and multifloral honey samples. Fourier transform near-infrared spectroscopy (FT-NIR), CCD near-infrared spectroscopy and PDA near-infrared spectroscopy were evaluated to quantitatively determine water content, fructose content and glucose content in honey. On the basis of partial-least square (PLS) regression, the models of honey were compared.

[Feasibility of using NIR spectroscopy to detect melamine in milk].

In the present study, 22 certified milk samples without melamine were collected, then 50 adulterated milk samples with added different content of melamine (0.1-1 500 mg x kg(-1)) were prepared. The near-infrared (NIR) spectra of these milk samples were measured.

[Analysis of NIR characteristic wavelengths for apple flesh firmness based on GA and iPLS].

In the present study, the fruit flesh firmness of apple was analyzed by near infrared (NIR) spectroscopy using an FT-NIR spectrometer. The sensitive spectral regions that provide the lowest prediction error were analyzed by different well-known variable selection methods, including dynamic backward interval partial least-squares (dynamic biPLS), sequential application of backward interval partial least-squares and genetic algorithm(dynamic biPLS & GA-PLS), and iterative genetic algorithm partial least-squares (iterative GA-PLS). Iterative GA-PLS, dynamic biPLS & GA-PLS led to a distinct reduction in the number of spectral data points with better predictive quality.

Clinical utility of an automated pupillometer for assessing and monitoring recipients of liver transplantation.

Pupil examination has been used as a basic measure in critically ill patients and has great importance for the prognosis and management of disease. An automated pupillometer is a computer-based infrared digital video system by which the accuracy and precision of the pupil examination are markedly improved. We conducted an observational study of pupil assessment with automated pupillometry in clinical liver transplantation settings, including pretransplant evaluations and posttransplant surveillance.

[Using GA-dOSC method to eliminate interference of peel with prediction of apple firmness based on near infrared diffuse reflection spectra].

In the present work, "Fuji" apples from Shandong Yantai were used to take the diffuse reflection spectra by FT-NIR PLS components (i.e., factors) were computed by nonlinear iterative partial least squares (NIPALS) and the number of latent factors (LV) was optimized by a leave-one-out cross-validation procedure on the calibration set.

[Improving apple fruit quality predictions by effective correction of Vis-NIR laser diffuse reflecting images].

In the present study, improved laser-induced light backscattering imaging was studied regarding its potential for analyzing apple SSC and fruit flesh firmness. Images of the diffuse reflection of light on the fruit surface were obtained from Fuji apples using laser diodes emitting at five wavelength bands (680, 780, 880, 940 and 980 nm). Image processing algorithms were tested to correct for dissimilar equator and shape of fruit, and partial least squares (PLS) regression analysis was applied to calibrate on the fruit quality parameter.

Long-term outcome of combined liver-kidney transplantation: a single-center experience in China.

Background/aims: To evaluate patient survival with combined liver-kidney transplantation (CLKT) and to identify factors of death in these patients.

Methodology: Among 554 cases of liver transplantations (LTs) performed between January 1999 and October 2006, there were 19 cases (3.43%) of CLKT recipients.

The performance of nu-support vector regression on determination of soluble solids content of apple by acousto-optic tunable filter near-infrared spectroscopy.

The nu-support vector regression (nu-SVR) was used to construct the calibration model between soluble solids content (SSC) of apples and acousto-optic tunable filter near-infrared (AOTF-NIR) spectra. The performance of nu-SVR was compared with the partial least square regression (PLSR) and the back-propagation artificial neural networks (BP-ANN). The influence of SVR parameters on the predictive ability of model was investigated.

Study of wavelet denoising in apple's charge-coupled device near-infrared spectroscopy.

Discrete wavelet transform was used to eliminate the noise in the charge-coupled device near-infrared (CCD-NIR) spectra of apple. The influence of three parameters (wavelet function, decomposition level, and threshold) on the predictive ability of the calibration model was investigated. The result showed that the db, sym, and bior wavelet families performed well, while the coif, dmey, and haar wavelets were not able to denoise effectively.

[Comparative proteomics research of apoptosis initiation induced by homoharringtonine in HL-60 cells].

Objective: To study the related proteins of apoptosis initiation induced by homoharringtonine (HHT) in HL-60 cells.

Methods: After establishment of an apoptosis initiation model induced by HHT in HL-60 cells, proteins of untreated and HHT treated HL-60 cells were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) two-dimensional electrophoresis respectively. The alteration protein spots were identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.

[A preliminary experience of treatment of severe aplastic anemia with murine anti-human CD3 T lymphocyte monoclonal antibody].

Objective: To investigate the efficacy of the specific murine anti-human CD(3) T lymphocyte monoclonal antibody (CD(3) MoAb) for severe aplastic anemia (SAA).

Methods: 13 SAA patients were chosen with a medium age of 22 years, including 4 untreated patients and 9 nonresponding to previous management. They were treated with 5 mg of CD(3) MoAb per day for a total 10 days.