CD39 Contributes to the Ability of Cell Invasion in Heterogeneity of Colorectal Cancer.

Tumour heterogeneity will accumulate and amplify during cell culture and passage, even if derived from the same strain. In the current study, multiple batches of colorectal cancer HCT116 and HT29 cell lines were obtained using different conditions of trypsin digestion and processed for RNA sequencing. CD39 was identified as a biomarker highly expressed in easily trypsin-digested cells compared to the undigestible ones.