Multi-omics analyses reveal bacteria and catalase associated with keloid disease.

Background: The pathology of keloid and especially the roles of bacteria on it were not well understood.

Methods: In this study, multi-omics analyses including microbiome, metaproteomics, metabolomic, single-cell transcriptome and cell-derived xenograft (CDX) mice model were used to explore the roles of bacteria on keloid disease.

Findings: We found that the types of bacteria are significantly different between keloid and healthy skin.

Decoding the molecular landscape of keloids: new insights from single-cell transcriptomics.

Keloids are a fibrotic disease caused by an excessive accumulation of extracellular matrix in the dermis; they have neoplasia-like properties of aggressive growth and high posttreatment recurrence rates. Therefore, it is imperative to gain additional insight into the pathobiology of keloid formation. Single-cell RNA sequencing (scRNA-seq) technology has brought data-driven innovation to understanding the pathogenesis of keloids by breaking the limitations of traditional sequencing technologies to resolve cell composition and to distinguish functional cell subtypes at an unprecedented resolution.

Deciphering the single-cell transcriptome network in keloids with intra-lesional injection of triamcinolone acetonide combined with 5-fluorouracil.

Objectives: Keloid is a highly aggressive fibrotic disease resulting from excessive extracellular matrix deposition after dermal injury. Intra-lesional injection of triamcinolone acetonide (TAC) in combination with 5-fluorouracil (5-FU) is a commonly used pharmacological regimen and long-term repeated injections can achieve sustained inhibition of keloid proliferation. However, the molecular mechanisms underlying the inhibitory effect on keloids remain insufficiently investigated.

Multi-omics integrative analysis reveals the role of tumor necrosis factor superfamily member 4 in keloidal scarring.

Objectives: To identify aberrantly expressed immune molecules in keloids and to explore their possible biologic significance.

Methods: Immune molecules with abnormal expression were identified based on immune gene sequencing of keloids, microarray datasets and high-throughput sequencing datasets and methylation microarray datasets from the Gene Expression Omnibus (GEO) database, and real-time quantitative PCR analysis.

Results: Upregulation of tumor necrosis factor superfamily member 4 (TNFSF4) in keloids was identified.

Histopathologic study of keloid vascular structures shows the vascular origin pattern of keloid subepidermal vascular network flaps.

Background: Keloid subepidermal vascular network flaps (KSVNFs) have achieved satisfactory results in clinical practice. Through this retrospective study, we further examined keloid vascular structure to better understand vascular origin pattern in KSVNFs.

Methods: Paraffin-embedded keloid tissues were stained for CD31.

Risk factors associated with keloid infections: A five-year retrospective study.

Keloid infections reduce patient-reported quality of life greatly. Characteristics and risk factors of keloid infections have not been thoroughly studied. So, a retrospective cohort study was conducted focusing on the potential risk factors, microbiologic cultures and histological findings.

Single-cell RNA sequencing reveals distinct immunology profiles in human keloid.

Keloids, characterized by skin fibrosis and excessive accumulation of extracellular matrix, remain a therapeutic challenge. In this study, we systematically capture the cellular composition of keloids by the single-cell RNA sequencing technique. Our results indicated that there are significant differences in most cell types present between 12 pairs of keloid and adjacent normal tissue.

Advances in the pathogenesis and clinical application prospects of tumor biomolecules in keloid.

Keloid scarring is a kind of pathological healing manifestation after skin injury and possesses various tumor properties, such as the Warburg effect, epithelial-mesenchymal transition (EMT), expression imbalances of apoptosis-related genes and the presence of stem cells. Abnormal expression of tumor signatures is critical to the initiation and operation of these effects. Although previous experimental studies have recognized the potential value of a single or several tumor biomolecules in keloids, a comprehensive evaluation system for multiple tumor signatures in keloid scarring is still lacking.

Giant pilomatrixoma with bullous appearance on the back: A case report and discussion of misdiagnosis.

Pilomatrixoma is a benign, asymptomatic skin tumor that forms from hair follicle matrix cells. Most are under 3 cm in diameter and occur on the head, face, or neck. Bullous appearance is very rare in pilomatrixoma.

Fabrication of cobaltous telluride and carbon composite as a promising carrier for boosting electro oxidation of ethylene glycol on palladium in alkaline medium.

The development of highly active and earth-rich electrocatalysts remains a formidable challenge for the commercialization of fuel cells. Herein, a composite carrier composed of cobaltous telluride (CoTe) and carbon (C) has been designed for the first time to enhance the electrocatalytic performance of palladium (Pd) nanoparticles (NPs) for the electro-oxidation of ethylene glycol (EG). Remarkably, the mass activity for the as-prepared Pd/CoTe-C catalyst during the ethylene glycol oxidation reaction (EGOR) is found to reach up to 3917.

Reliability and Validity of the Chinese Version of the Lymphedema Quality of Life Questionnaire.

Objective To study the reliability and validity of the Chinese version of the Lymphedema Quality of Life Questionnaire (LYMQOL) in lymphedema patients. Methods LYMQOL was translated into Chinese. The Chinese version of the LYMQOL was distributed with the official Wechat account "Lymphedema Channel" to lymphedema patients who were recruited from October 28 , 2020 to February 23, 2021.

Synthesis of PtRu alloy nanofireworks as effective catalysts toward glycerol electro-oxidation in alkaline media.

Electro-oxidation of glycerol is a key anodic reaction in direct alcohol fuel cell (DAFCs). Exploring the cost-effective nanocatalysts for glycerol oxidation reaction (GOR) is very important for the development of DAFC, but it is still challenging. In this paper, nanofirework-like PtRu alloy catalyst was successfully synthesized and used for GOR in alkaline medium.

Application and prospect of adipose stem cell transplantation in treating lymphedema.

Background: Lymphedema is a chronic, debilitating and incurable disease that affects 0.13%-2% of the global population. Emerging evidence indicates that adipose-derived stem cells (ADSCs) might serve as suitable seed cells for lymphatic tissue engineering and lymphedema therapy.

Effect of enterotoxin on gastric cancer cells SGC7901 which highly expressed claudin-4 protein.

Aim: To investigate the effects of enterotoxin (CPE) on gastric cancer cells which highly expressed claudin-4 (CL4) protein.

Methods: In this study, we detected expression of CL4 protein in different gastric cancer cell lines. Then, we investigated the effects of CPE on SGC7901 cells which highly expressed CL4 protein and the effects of CPE on subcutaneous tumor in nude mice models.