Optimization of Enzymatic Hydrolysis and Fermentation Processing for Set-Type Oat Yogurt with Favorable Acidity and Coagulated Texture.

The key role of enzymatic hydrolysis and fermentation in the sensory quality of set yogurt made from whole oats was demonstrated. The optimal process was established by the orthogonal and response surface methodology based on the acidity, textural, and rheological properties. The results indicated that the enzymatic hydrolysis appropriately consisted of liquefaction with 12 U/mL α-amylase at 70 °C and pH 6.

Heterologous expression of P9 from Akkermansia muciniphila increases the GLP-1 secretion of intestinal L cells.

Glucagon-like peptide-1(GLP-1) is an incretin hormone secreted primarily from the intestinal L-cells in response to meals. GLP-1 is a key regulator of energy metabolism and food intake. It has been proven that P9 protein from A.

Homologous Overexpression of Tyrosinase in and Its Application in Glycinin Cross-Linking.

Tyrosinase is capable of oxidizing tyrosine residues in proteins, leading to intermolecular protein cross-linking, which could modify the protein network of food and improve the texture of food. To obtain the recombinant tyrosinase with microbial cell factory instead of isolation tyrosinase from the mushroom , a TYR expression cassette was constructed in this study. The expression cassette was electroporated into Rut-C30 and integrated into its genome, resulting in a recombinant strain C30-TYR.

A Ropy Exopolysaccharide-Producing Strain Bi-OTA128 Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice.

Inflammatory bowel disease (IBD) is a chronic disease associated with overactive inflammation and gut dysbiosis. Owing to the beneficial effects of bifidobacteria on IBD treatment, this study aimed to investigate the anti-inflammation effects of an exopolysaccharide (EPS)-producing strain Bi-OTA128 through a dextran sulfate sodium (DSS)-induced colitis mice model. treatment improved DSS-induced colitis symptoms and maintained intestinal barrier integrity by up-regulating MUC2 and tight junctions' expression.

Regulates the Heterogeneity Driven by the Acid Tolerance Response in L9.

The acid tolerance of lactic acid bacteria is crucial for their fermentation and probiotic functions. Acid adaption significantly enhances the acid tolerance of strains, and the phenotypic heterogeneity driven by the acid tolerance response (ATR) contributes to this process by providing a selective advantage in harsh environments. The mechanism of heterogeneity under the ATR is not yet clear, but individual gene expression differences are recognized as the cause.

Up-regulation of sortase-dependent pili in Bifidobacterium longum BBMN68 in response to bile stress enhances its adhesion to HT-29 cells.

Adhesion to gastrointestinal tract is crucial for bifidobacteria to exert their probiotic effects. Our previous work found that bile salts significantly enhance the adhesion ability of Bifidobacterium longum BBMN68 to HT-29 cells. In this study, trypsin-shaving and LC-MS/MS-based surface proteomics were employed to identify surface proteins involved in bile stress response.

Biofilm-Forming Capacity and Drug Resistance of Different Subgroups Associated with Bacterial Vaginosis.

Bacterial vaginosis (BV) is the most common infection of the lower reproductive tract among women of reproductive age. Recurrent infections and antibiotic resistance associated with biofilms remain significant challenges for BV treatment. species are commonly found in women with and without BV, indicating that genetic differences among isolates may distinguish pathogenic from commensal subgroups.

An underlying mechanism for MleR activating the malolactic enzyme pathway to enhance acid tolerance in L9.

Tolerance to acid stress is a crucial property of probiotics against gastric acids. The malolactic enzyme pathway is one of the most important acid resistance systems in lactic acid bacteria. It has been reported that the malolactic enzyme pathway was regulated by the transcriptional regulator, MleR.

Molecular Organization and Functional Analysis of a Novel Plasmid-Borne Gene Cluster from YC41.

Capsular polysaccharide (CPS) can tightly attach to bacterial surfaces and plays a critical role in protecting microorganisms from environmental stresses. However, the molecular and functional properties of some plasmid-borne gene clusters are poorly understood. In this study, comparative genomics of the draft genomes of 21 Lactiplantibacillus plantarum strains revealed that the specific gene cluster for CPS biosynthesis was observed only in the 8 strains with a ropy phenotype.

Prevalence, antimicrobial susceptibility, and antibiotic resistance gene transfer of Bacillus strains isolated from pasteurized milk.

Pasteurization is carried out in dairy industries to kill harmful bacteria present in raw milk. However, endospore-forming bacteria, such as Bacillus, cannot be completely eliminated by pasteurization. In this study, a total of 114 Bacillus strains were isolated from 133 pasteurized milk samples.

The plasmid-encoded lactose operon plays a vital role in the acid production rate of during milk beverage fermentation.

is used extensively in the fermented milk-beverage industry as a starter culture. Acid production capacity during fermentation is the main criterion for evaluating starters although it is strain-dependent. In this study, the acid production rates of 114 strains were determined and then classified into high acid (HC), medium acid (MC), and low acid (LC) groups.

Unraveling the mechanism of raffinose utilization in Ren by transcriptomic analysis.

Unlabelled: In the gastrointestinal tract, some dietary carbohydrates, such as xylose, raffinose and arabinose, are able to stimulate the growth of and . In this study, the growth rate of Ren in raffinose was 0.91 ± 0.

L-Malic Acid Protects L9 from Glycodeoxycholic Acid Stress via the Malolactic Enzyme Pathway.

Bile stress tolerance is a crucial characteristic of probiotics for surviving in the human gastrointestinal tract. The mechanism underlying the effect of l-malic acid on enhancing the glycodeoxycholic acid (GDCA) tolerance of L9 was investigated herein. Bile tolerance specificity assays revealed that L9 was more sensitive to GDCA than to taurocholic acid, glycocholic acid, and taurodeoxycholic acid.

Anti-inflammatory effect of Bifidobacterium animalis subsp. lactis A6 on DSS-induced colitis in mice.

Aims: This study aimed to investigate the protective effect of Bifidobacterium animalis subsp. lactis A6 on dextran sodium sulphate (DSS)-induced colitis in C57BL/6J mice.

Methods And Results: Mice were randomly divided into three groups (n = 8 per group).

Transcriptome analysis revealed growth phase-associated changes of a centenarian-originated probiotic Bifidobacterium animalis subsp. lactis A6.

Background: The physiology and application characteristics of probiotics are closely associated with the growth phase. Bifidobacterium animalis subsp. lactis A6 is a promising probiotic strain isolated from the feces of a healthy centenarian in China.

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of in Raw Milk.

Raw milk is susceptible to microbial contamination during transportation and storage. producing heat-resistant enzymes have become the most common and harmful psychrophilic microorganisms in the cold chain logistics of raw milk. To rapidly detect in raw milk, the protease gene X was selected as a detection target to construct a set of primers with strong specificity, and a loop-mediated isothermal amplification (LAMP) assay was established.

GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice.

Ulcerative colitis (UC) is a relapsing and remitting inflammatory disease. Probiotics have a potential beneficial effect on the prevention of UC onset and relapse in clinical trials. GG ( GG) have shown clinical benefits on UC patients, however, the precise mechanisms are unknown.

Homologous Over-Expression of Chain Length Determination Protein EpsC Increases the Molecular Weight of Exopolysaccharide in 05-34.

In , EpsC is a polysaccharide co-polymerase which is involved in determining the chain length of EPS synthesized by the Wzx/Wzy-dependent pathway. Our previous study found that there was a positive correlation between transcription level of and molecular weight of EPS in 05-34. To further investigate the effects of EpsC on EPS biosynthesis, this gene was over-expressed in 05-34 in this study.

Correlation in Causality: A Progressive Study of Hierarchical Relations within Human and Organizational Factors in Coal Mine Accidents.

It has been revealed in numerous investigation reports that human and organizational factors (HOFs) are the fundamental causes of coal mine accidents. However, with various kinds of accident-causing factors in coal mines, the lack of systematic analysis of causality within specific HOFs could lead to defective accident precautions. Therefore, this study centered on the data-driven concept and selected 883 coal mine accident reports from 2011 to 2020 as the original data to discover the influencing paths of specific HOFs.

A Novel Major Pilin Subunit Protein FimM Is Involved in Adhesion of BBMN68 to Intestinal Epithelial Cells.

Adhesion to the gastrointestinal tract is considered to be important for bifidobacteria to colonize the human gut and exert their probiotic effects. Some cell surface proteins of bifidobacteria, known as adhesins, play critical roles in the binding to host cells or the extracellular matrix (ECM). To elucidate the mechanisms associated with the adhesion of BBMN68, a centenarian originated potential probiotic, PSORTdb was employed to identify putative extracellular localized proteins in the BBMN68.

Expression, purification, and characterization of phospholipase B1 from in .

is an important fungal pathogen that causes a wide variety of human infections, ranging from mucocutaneous infections to life-threatening systemic infections. Phospholipase B1 (PLB1) has been reported to be directly responsible for pathogenicity and is likely to be involved in the early steps of host invasion. Therefore, PLB1 could be a potential marker for diagnosis of infection.

The N-terminal domain of rhamnosyltransferase EpsF influences exopolysaccharide chain length determination in 05-34.

Glycosyltransferases are key enzymes involved in the assembly of repeating units of exopolysaccharides (EPS). A glycosyltransferase generally consists of the N-terminal and the C-terminal domain, however, the functional role of these domains in EPS biosynthesis remains largely unknown. In this study, homologous overexpression was employed to investigate the effects of EpsF, a truncated form of rhamnosyltransferase EpsF with only the N-terminal domain, on EPS biosynthesis in 05-34.

Corrigendum: Global Transcriptomic Analysis and Function Identification of Malolactic Enzyme Pathway of L9 in Response to Bile Stress.

[This corrects the article DOI: 10.3389/fmicb.2018.