Publications by authors named "Zhengxian He"
The objective of study was to investigate the in vitro suppressive effect of angelica polysaccharide (APS) on human cytomegalovirus-induced apoptosis via direct infection in CHRF-288-11 cells. HCMV AD169 directly infected CHRF-288-11 were cultured in vitro, APS in different doses were added on day 3 after the infection of virus. Cells of every group were collected at different time points.
View Article and Find Full Text PDF[Human cytomegalovirus induces apoptosis of human promyelocytic leukemic cells via direct infection in vitro].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
February 2007
The study was purposed to investigate the proliferation and the suppression effect of human cytomegalovirus (hCMV) on human promyelocyte cell line HL-60, and to study whether hCMV can induce apoptosis of HL-60 via direct infection in vitro and its mechanism. Promyelocyte cell line HL-60 and hCMV AD169 strain were co-cultured. PCR was used to detect the direct infection of HL-60 cells by hCMV IEA expression.
View Article and Find Full Text PDF[Clinical investigation on the treatment of HCMV hepatitis in children].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
March 2004
Objective: To investigate the effective therapeutic method of human cytomegalovirus (HCMV) hepatitis in children.
Methods: Twenty-five children with HCMV hepatitis were randomly assigned to a treated group (n=13) or a control group (n=12). Both groups were treated with prednisone, glucurone, luminal and Xiaoyanlidanpian.
[Correlation between plasma leptin level and premature infant weight loss].
Zhonghua Er Ke Za Zhi
February 2004
Article Synopsis
- Leptin is a hormone produced by fat cells that plays a role in regulating body weight and energy balance, and this study focuses on its levels in premature infants during the neonatal period.
- A radioimmunoassay was used to measure plasma leptin concentrations in 26 premature infants, with blood samples taken at birth and every two days until weight was regained.
- Results indicated a significant positive correlation between body weight loss and plasma leptin levels in the infants, with both dropping to low levels within the first week after birth.
[Human cytomegalovirus aggravates apoptosis of human megakaryocytes via direct infection in vitro].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
February 2004
The megakaryocyte and platelet lineage may be one of the major sites of human cytomegalovirus (HCMV) infection. However, whether HCMV aggravates apoptosis in normal megakaryocytes was not well investigated. Megakaryocytic cell line CHRF-288-11 and HCMV AD 169 strain were co-cultured in this study.
View Article and Find Full Text PDFObjective: To investigate the mechanism and the suppression effect of human cytomegalovirus (HCMV) on hematopoietic system.
Methods: Semi-solid culture system was used to observe the effect of HCMV AD169 strain on colony forming unit granulocyte/macrophage (CFU-GM), CFU-erythroid (CFU-E), CFU-multipotent (CFU-Mix) and CFU-megakaryocyte (CFU-MK) growth. The techniques of in situ polymerase chain reaction (IS-PCR) and polymerase chain reaction (PCR) were used to demonstrate the existence of HCMV DNA in the colony cells of cultured CFU-GM, CFU-Mix, CFU-MK and CFU-E, respectively.
[Detection of human cytomegalovirus infection by FQ-PCR technique and its application in the diagnosis and treatment of HCMV infected children].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
June 2002
Background: To detect quantitatively HCMV DNA in peripheral blood leukocytes to monitor the status of HCMV infection, evaluate the effectiveness of antiviral treatment with ganciclovir (GCV) combined with intravenous immunoglobulin (IVIG) and find out the relationship among the HCMV DNA levels, the state of infection and the clinical outcome.The long-term goal of the study was to establish a molecular diagnostic standard for HCMV infection in children.
Methods: 45 cases of suspected HCMV-infected children were examined by PCR, ELISA and fluorescent quantitative (FQ)-PCR, respectively.