Detecting Bacteria with Ultralow Concentrations by Enzymatic Cascade Reaction-Amplifying Strategy.

Bacteria can cause infectious diseases even at ultralow concentrations (<1 CFU/mL). It is important to rapidly identify bacterial contamination at ultralow concentrations. Herein, FITC-labeled gelatinase-sensitive nanoparticles (GNPs@FITCs) and NFM@GNP@FITCs are designed and fabricated as ultralow concentration bacteria detection platforms based on an enzymatic cascade reaction-amplifying strategy.

Is conventional magnetic resonance imaging superior to radiography in the functional integrity evaluation of anterior cruciate ligament in patients with knee osteoarthritis?

Background: The functional integrity of the anterior cruciate ligament (ACL) influences surgical decision-making in patients with knee osteoarthritis (KOA). This study aimed to compare the diagnostic value of radiography and magnetic resonance imaging (MRI) in determining the functional status of ACL.

Methods: We analyzed 306 knees retrospectively using preoperative hip-to-ankle anteroposterior standing (APS) radiographs, anteroposterior (AP) and lateral knee radiographs, AP valgus stress (VS) force radiographs, and standard orthogonal MRI.

Factors that impact the patellofemoral contact stress in the TKA: a review.

Abnormal retro patellar stress is believed to contribute to patellofemoral complications after total knee arthroplasty (TKA), but the causal link between TKA and patellofemoral contact stress remains unclear. By reviewing the relevant studies, we found that both TKA implantation and additional patellar resurfacing increase retro patellar pressure. The rotation and size of the femoral component, thickness and position of the patellar component, installation of the tibial component, prosthesis design and soft tissue balance further influence patellofemoral stress.

Pressure Attenuation Law of Low-Frequency Pulse Pressure Flooding and Its Influence on Oil Recovery.

Pulse water injection is widely used in tertiary oil recovery. This study aims to reduce the pulse frequency, control the pulse frequency at 0.033∼0.

Relationship between patellofemoral finite helical axis and femoral trans-epicondylar axis using a static magnetic resonance-based methodology.

Background: To manage patellofemoral joint disorders, a complete understanding of the in vivo patellofemoral kinematics is critical. However, as one of the parameters of joint kinematics, the location and orientation of the patellofemoral finite helical axis (FHA) remains unclear. The purpose of this study is to quantify the location and orientation of the patellar FHA, both in vivo and non-invasively at various flexion angles, and evaluate the relationship of the FHA and the trans-epicondylar axis (TEA).

Correlation between femorotibial pressure and joint gap in osteoarthritis patients with varus deformity: an intraoperative measurement using sensor device in primary posterior-stabilized total knee arthroplasty.

Background: The normal femorotibial pressure and its variation under different gap values remain unknown. Thus, for the purpose of improving soft-tissue balancing in total knee arthroplasty (TKA), a load-sensor device was used to measure femorotibial pressures. More specifically, the aim of this study was to analyze the trend in pressure changes.

Surface Functional Nanofiber Membrane for Ultrasensitive and Naked-Eye Visualization of Bacterial Concentration.

Bacterial contamination in water is a serious health risk to human beings, so it is very important to realize the point-of-care (POC) bacterial detection in water. However, the traditional bacterial detection methods are time-consuming, professional- and equipment-dependent, and do not meet the needs of POC detection. There is a pressing need to develop a platform for POC bacterial detection to defeat the increasing risk of bacterial infections.

Research Methods and Progress of Patellofemoral Joint Kinematics: A Review.

Patellofemoral pain syndrome has a high morbidity, and its pathology is closely associated with patellofemoral joint kinematics. A series of and studies have been conducted to explore patellofemoral kinematics, and the findings are relevant to the diagnosis, classification, and management of patellofemoral diseases and even the whole knee joint. However, no definite conclusion on normal patellofemoral kinematics has been established.

Dynamic layer-by-layer films on nanofiber membrane: a platform for ultra-sensitive bacterial concentration detection.

Herein, a new platform was established for ultra-sensitive bacterial concentration detection. The sensing system had a linear relationship with the logarithm of bacterial concentration from 1 × 101 to 1 × 105 CFU mL-1 within 5 min. Moreover, the platform showed excellent consistency compared with the traditional standard method against practical samples.

Local autoantigen expression as essential gatekeeper of memory T-cell recruitment to islet grafts in diabetic hosts.

It is generally believed that inflammatory cues can attract noncognate, "bystander" T-cell specificities to sites of inflammation. We have shown that recruitment of naive and in vitro activated autoreactive CD8⁺ T cells into endogenous islets requires local autoantigen expression. Here, we demonstrate that absence of an autoantigen in syngeneic extrapancreatic islet grafts in diabetic hosts renders the grafts "invisible" to cognate memory (and naive) T cells.

Microbial community of granules in expanded granular sludge bed reactor for simultaneous biological removal of sulfate, nitrate and lactate.

This study studied the cultivation of granules from an expanded granular sludge bed reactor that simultaneously transforms sulfates, nitrates, and oxygen to elementary sulfur, nitrogen gas, and carbon dioxides, respectively. The living cells accumulate at the granule outer layers, as revealed by the multicolor staining and confocal laser scanning microscope technique. The microbial community comprises sulfate-reducing bacteria (SRB, Desulfomicrobium sp.

Simultaneous biological removal of sulfur, nitrogen and carbon using EGSB reactor.

High-rate biological conversion of sulfide and nitrate in synthetic wastewater to, respectively, elemental sulfur (S(0)) and nitrogen-containing gas (such as N(2)) was achieved in an expanded granular sludge bed (EGSB) reactor. A novel strategy was adopted to first cultivate mature granules using anaerobic sludge as seed sludge in sulfate-laden medium. The cultivated granules were then incubated in sulfide-laden medium to acclimate autotrophic denitrifiers.

Surfactant as an airway smooth muscle relaxant.

A variety of clinical and experimental evidence indicates that surfactant may be important in the pathogenesis and treatment of asthma. The purpose of this study was to determine the pharmacologic effect of pulmonary surfactant and its major lipid and protein constituents on bronchial smooth muscle. First-generation bronchi from male Sprague-Dawley rats were contracted with methacholine and exposed to two kinds of surfactant: whole rat surfactant and two bovine surfactant extracts in clinical use.