Publications by authors named "Zhengrong Gao"

Background: Mpox has spread to many countries around the world. While the existing live attenuated mpox vaccines are effective, advances in 21st century technologies now enable the development of vaccines with more specific antigens, clearer mechanisms, and more controllable side effects.

Methods: We systematically evaluated the immunogenicity and protective efficacy of the A35R, M1R and B6R antigens of the mpox virus (MPXV).

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  • Blood-based mitochondrial bioenergetic profiling is a practical and low-risk method for studying mitochondrial function and energy metabolism using human blood samples.
  • The study employs two respirometric techniques to assess mitochondrial bioenergetics in peripheral blood mononuclear cells and platelets, revealing that female participants exhibit significantly higher respiration rates than male participants, even after adjusting for other variables.
  • Findings highlight the importance of considering sex differences in mitochondrial bioenergetic profiles, particularly in the context of human health and aging research.
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  • A study compared the effects of a new sonic irrigation system called Easydo Activator (EA) on sealer penetration at the root apex to traditional irrigation methods: needle irrigation (NI) and passive ultrasonic irrigation (PUI).
  • Forty-two single-rooted teeth were divided into three groups for different irrigation treatments, followed by analysis using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) to assess sealer penetration and smear layer removal.
  • The results showed that EA outperformed both NI and PUI in terms of greater sealer penetration area, depth, and lower smear layer scores, suggesting it could enhance clinical root canal therapy.
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The global outbreak of the mpox virus (MPXV) in 2022 highlights the urgent need for safer and more accessible new-generation vaccines. Here, we used a structure-guided multi-antigen fusion strategy to design a 'two-in-one' immunogen based on the single-chain dimeric MPXV extracellular enveloped virus antigen A35 bivalently fused with the intracellular mature virus antigen M1, called DAM. DAM preserved the natural epitope configuration of both components and showed stronger A35-specific and M1-specific antibody responses and in vivo protective efficacy against vaccinia virus (VACV) compared to co-immunization strategies.

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Objective: To investigate the efficacy of treating patients with HIV-positive osteonecrosis of the femoral head using drilled decompression autologous bone marrow and allogeneic bone grafting.

Methods: 40 patients (44 hips) with early osteonecrosis of the femoral head treated by drilling decompression autologous bone marrow and allogeneic bone grafting since October 2015 were retrospectively analyzed, among which 20 patients (24 hips) were HIV-positive patients with early osteonecrosis of the femoral head, 16 males and 4 females, age 22-43 years, average 39.6 ± 10.

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Unlabelled: The article has been withdrawn at the request of the author of the journal Current HIV Research (CHIVR). Bentham Science apologizes to the readers of the journal for any inconvenience this may have caused. The Bentham Editorial Policy on Article Withdrawal can be found at https://benthamscience.

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SARS-CoV-2 variants with severe immune evasion are a major challenge for COVID-19 prevention, especially the circulating Omicron XBB/BQ.1.1/BF.

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Vaccination with different vaccines has been implemented globally to counter the continuous COVID-19 pandemic. However, the vaccine-elicited antibodies have reduced efficiency against the highly mutated Omicron sub-variants. Previously, we developed a protein subunit COVID-19 vaccine called ZF2001, based on the dimeric receptor-binding domain (RBD).

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Kangfuxin (KFX) shows potential in wound healing, but its role in socket healing is unclear. This research finds increased bone mass, mineralization, and collagen deposition in KFX-treated mice. Mouse bone marrow mesenchymal stem cells, human periodontal ligament stem cells (hPDLSCs), and human dental pulp stem cells (hDPSCs) are treated with KFX under osteogenic induction.

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  • - The article discusses the challenges of treating jaw-bone defects caused by diseases, emphasizing the need for better reconstruction methods through bone tissue engineering using oral cavity-derived stem cells.
  • - It reviews current research on the effectiveness of various types of stem cells and jaw-bone defect models, highlighting their respective advantages and disadvantages.
  • - The conclusion suggests that the choice of cell type and animal model should align with the specific goals of research and the nature of the disease, providing groundwork for future tissue engineering studies.
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Objective: HIV patients are prone to infection and difficult to treat, which mainly manifests itself in decreased CD4 T cells in the body. Therefore, the predictive value of lymphocyte count and hemoglobin for CD4 levels in HIV patients was discussed in the prospective study.

Methods: 125 HIV patients (aged >18 or < 80 years) were recruited.

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  • Mitochondrial bioenergetics, which are essential for cellular energy production, are altered in patients with Alzheimer's disease, specifically affecting those with normal cognition, mild cognitive impairment, and dementia.
  • The study measured mitochondrial respiration differences in blood cells from participants, finding that those with dementia showed significantly lower bioenergetics compared to those with normal cognition.
  • Analysis of lipid metabolites in the participants' plasma revealed that glycocholic acid (GCA) was lower and linoleic acid (LA) was higher in dementia patients, suggesting these lipids may influence energy metabolism and cognitive status.
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Background: The Oral Health-related Quality of Life (OHRQoL) is a multi-dimensional concept commonly used to examine the impact of health status on quality of life, and the Oral Health Impact Profile-14 (OHIP-14) questionnaire is a good self-assessment tool. This study was designed to investigate the factor structure of the OHIP-14 scale Chinese version, measurement invariance and latent mean differences across genders among college students.

Methods: The online survey was completed by 919 college students.

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  • Researchers discovered that mitochondrial dysfunction in blood cells can indicate cognitive decline in Alzheimer's disease (AD) patients.
  • The study found that people with dementia had the lowest mitochondrial function in their blood cells, while those with mild cognitive impairment (MCI) showed higher oxygen use compared to healthy individuals.
  • Blood-based assessments of mitochondrial function could serve as a non-invasive method to track bioenergetic changes related to AD risk and progression over time.
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Bone tissue engineering (BTE) is a promising method for the repair of difficult-to-heal bone tissue damage by providing three-dimensional structures for cell attachment, proliferation, and differentiation. Traditional Chinese medicine (TCM) has been introduced as an effective global medical program by the World Health Organization, comprising intricate components, and promoting bone regeneration by regulating multiple mechanisms and targets. This study outlines the potential therapeutic capabilities of TCM combined with BTE in bone regeneration.

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The immunological response occurring during periapical inflammation includes expression of nucleotide binding oligomerization domain containing 2 and hepcidin. Nucleotide binding oligomerization domain containing 2 deficiency increases infiltration of inflammatory cells close to alveolar bone. Hepcidin has an important role in iron metabolism affecting bone metabolism.

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  • The study investigates the impact of Mycobacterium leprae infection on bone remodeling, particularly focusing on the role of osteocytes in leprosy-related bone formation and loss.
  • Researchers treated osteocyte-like cells with N-glycosylated muramyl dipeptide (N.g MDP) and found significant changes in messenger RNAs and circular RNAs, identifying eight key genes linked to osteogenic differentiation.
  • The findings suggest a unique circRNA-miRNA-mRNA network that could lead to new therapeutic targets for leprosy by better understanding how N.g MDP affects osteocyte function and bone health.
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Periodontitis is a globally prevalent disease that imposes a functional and aesthetic burden on patients. The oral microbiome influences human health. The aim of this study was at assessing gender variation in the subgingival bacterial microbiome of elderly patients with initial periodontitis and to determine the causes of this variation.

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To provide reliable molecular markers and effective therapeutic targets for chondrosarcoma and glioma.Gene Set Enrichment (GSE) 29745 and GSE48420 were downloaded from the Gene Expression Omnibus (GEO) database. Differently expressed genes (DEGs) were identified by the GEO2R.

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  • The study investigates gender differences in the oral microbiomes of youths with severe periodontitis by analyzing subgingival plaque samples from 17 patients (11 males, 6 females).
  • Using 16S rRNA gene sequencing, researchers compared microbiome composition and diversity while applying statistical analyses like LEfSe to identify specific bacteria in each group.
  • Results revealed that males had higher overall microbial diversity and different dominant bacteria compared to females, with implications for understanding how these differences may affect immune responses and periodontitis progression.
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  • * This study compared the effects of a Mediterranean diet, linked to better health, and a Western diet, linked to poorer outcomes, on mitochondrial bioenergetics in three brain regions of middle-aged macaques.
  • * Results showed that the Mediterranean diet preserved significant differences in mitochondrial function across brain regions, while the Western diet led to reduced bioenergetic distinctions and positive correlations with increased blood glucose and insulin levels in specific regions associated with Alzheimer's and diabetes.
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Paxillin (PXN) is a protein involved in numerous physiological processes, and its presence is closely related to the occurrence and development of many types of tumors. However, no studies have analyzed PXN from a pan-cancer perspective. We analyzed expression, immune cell infiltration, prognosis, and biological function across different types of tumors included in The Cancer Genome Atlas and Gene Expression Omnibus datasets.

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Bone healing in tooth extraction sockets occurs in a complex environment containing saliva and many microorganisms and is affected by many factors. Endoplasmic reticulum (ER) stress affects bone metabolism, but the role of ER stress in bone healing after tooth extraction remains unclear. We utilized a rat tooth extraction model, in which we promoted wound healing by using salubrinal to regulate the ER stress response.

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The mechanisms underlying the two-way relationship between diabetes mellitus (DM) and periodontitis are unclear. We examined a possible effect of galectin-3 (Gal-3), a factor in DM and bone metabolism, on periodontitis with or without DM. Using enzyme-linked immunosorbent assay, we detected saliva Gal-3 in patients with periodontitis, with or without type 2 diabetes mellitus (T2DM).

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