CT imaging character of different brain regions in different ages of Diannan small-ear pigs.

Objective: To collect and establish normal data of the brain regions of Diannan small-ear (DSE pigs, basically throughout measuring and comparing computed tomography values of the barin.

Methods: 12 ordinary DSE pigs were divided into juvenile, adult, old groups based on the physiological ages (n=4) A SOMATOM Definition AS 64-row 128-slice 4D spiral CT scanner (SOMATOM Definition AS, Germany) was used to collect CT images of DSE pigs, record and analyze the scanning data.

Results: Compared with the juvenile group, the CT values of the right frontal lobe, right parietal lobe, right temporal lobe, left temporal lobe, and right occipital lobe were significantly higher in the elderly group.

Application prospects of cerebrospinal fluid DIA techniques in identifying key regulatory molecules for mental disorders in encephalitis.

At present, data independent acquisition (DIA) protein spectrum detection technology is one of the most attractive mass spectrometry acquisition techniques, which once led the new development of quantitative proteomics. Its application fields include the screening of clinical disease markers, the study of action mechanism, the study of drug targets and so on. DIA has been wide-ranging used in clinic because of its high throughput, high resolution and high reproducibility.

[Determination of total phthalates in perfume and their exposure assessment].

A novel method for rapid screening of phthalates (PAEs) in perfumes was developed. The PAEs were hydrolyzed to phthalic acid (PA), and the PA in the acidified solution was extracted with tributyl phosphate (TBP) which was detected by high performance liquid chromatography-diode array detection (HPLC-DAD). Meanwhile exposure dose to PAEs was estimated by the percentage of a topically applied dose that permeates the skin.

Determination of total phthalate in cosmetics using a simple three-phase sample preparation method.

A simple sample preparation method requiring minimal organic solvents is proposed for the determination of the total phthalate content in cosmetics by high-performance liquid chromatography-tandem mass spectrometry. The hydrolysis of phthalates and purification of interfering substances were performed in a three-phase system that included an upper n-hexane phase, a middle ethanol phase, and a lower aqueous alkali solution. This three-phase system utilized an incremental purification strategy.

Determination of chlorophenols in water using dispersive liquid-liquid microextraction coupled with water-in-oil microemulsion electrokinetic chromatography in normal stacking mode.

The current routes to couple dispersive liquid-liquid microextraction with capillary electrophoresis are the evaporation of water immiscible extractants and the back-extraction of analytes. In this study, a new methodology for this combination using water-in-oil microemulsion electrokinetic chromatography coupled with normal stacking mode on-line sample concentration was developed to analyze chlorophenols in water samples. The analytes were extracted with tributyl phosphate and the extractant dilution (3×) was directly injected into an electrophoresis buffer (7.

Chiral N,N'-dioxide-Sc(NTf) complex-catalyzed asymmetric bromoamination of chalones with N-bromosuccinimide as both bromine and amide source.

The catalytic asymmetric bromoamination of chalcones with N-bromosuccinimide as both bromine and amide source was realized by using a chiral N,N'-dioxide-Sc(NTf) complex as an efficient catalyst. The corresponding chiral bromoamination products were obtained in high yields with high dr and good ee values (up to 92% yield, 93 : 7 dr and 97% ee) under mild reaction conditions.

The N,N'-dioxide/Ni(II)-catalyzed asymmetric inverse-electron-demand hetero-Diels-Alder reaction of methyleneindolinones with hetero-substituted alkenes.

The highly efficient catalytic asymmetric inverse-electron-demand hetero-Diels-Alder reaction of methyleneindolinones with hetero-substituted alkenes has been accomplished under mild reaction conditions. In the presence of chiral N,N'-dioxide/Ni(II) complexes, a wide range of optically active dihydropyran-fused indoles were obtained in up to 99% yield, >95 : 5 dr and 99% ee.

Acute PAR2 activation reduces GABAergic inhibition in the spinal dorsal horn.

We investigated the mechanism underlying inhibition of spinal dorsal horn GABAergic neurotransmission to elucidate the role of protease-activated receptor-2 (PAR2). Initially, we confirmed that PAR2 agonist SL-NH(2) applied intrathecally produced mechanical hyperalgesia. Then we performed patch-clamp experiments in substantia gelatinosa neurons of spinal cord slice, and found that spontaneous inhibitory post-synaptic currents (sIPSCs) were significantly decreased in both frequency and amplitude when neurons were incubated with PAR2 agonist SL-NH(2) for a brief time period (2 min).