A novel strategy to improving Rhodobacter azotoformans denitrification efficiency: Insight into the role of a two-component system NtrX/Y in denitrification regulation.

Transcription factors (TFs) can manage the coordinated expression of genes clusters or multiple genes. TF was used to improve bacterial denitrification ability in this study. During denitrification, the ntrY of R.

Ammonia assimilation: A double-edged sword influencing denitrification of Rhodobacter azotoformans and for nitrogen removal of aquaculture wastewater.

NO-N and NH-N are two prevalent nitrogenous pollutants in aquaculture wastewater posing a significant health risk to aquatic animals. R. azotoformans ATCC17025 can rapidly denitrify to remove NO-N, assimilating NH-N.

Improving CoQ productivity by strengthening glucose transmembrane of Rhodobacter sphaeroides.

Background: Several Rhodobacter sphaeroides have been widely applied in commercial CoQ production, but they have poor glucose use. Strategies for enhancing glucose use have been widely exploited in R. sphaeroides.

Improving microbial bioremediation efficiency of intensive aquacultural wastewater based on bacterial pollutant metabolism kinetics analysis.

How to effectively bioremediate aquacultural wastewater using microbes is an urgent issue for the application of aquaculture beneficial microorganisms. Purple non-sulfur bacteria (PNSB) are beneficial in preventing related pollution in aquaculture applications. An autochthonous PNSB Rhodobacter sphaeroides was employed in this study to explore an effective bioremediation strategy of aquacultural wastewater.

Biopotentiality of High Efficient Aerobic Denitrifier Bacillus megaterium S379 for Intensive Aquaculture Water Quality Management.

Excessive nitrite accumulation is a very tough issue for intensive aquaculture. A high efficient aerobic denitrifier Bacillus megaterium S379 with 91.71±0.

Biochemical analysis and the preliminary crystallographic characterization of D-tagatose 3-epimerase from Rhodobacter sphaeroides.

Background: D-Tagatose 3-epimerase epimerizes D-fructose to yield D-psicose, which is a rare sugar that exists in small quantities in nature and is difficult to synthesize chemically. We aim to explore potential industrial biocatalysts for commercial-scale manufacture of this rare sugar. A D-tagatose 3-epimerase from Rhodobacter sphaeroides (RsDTE) has recently been identified as a D-tagatose 3-epimerase that can epimerize D-fructose to yield D-psicose with a high conversion rate.

Improving fermented quality of cider vinegar via rational nutrient feeding strategy.

This work aimed to find a rational nutrient feeding strategy for cider vinegar fermentation based on adequate information on the nutritional requirement of acetic acid bacteria. Through single nutrient lack experiment assay, necessary nutrient recipe for Acetobacter pasteurianus CICIM B7003 in acetous fermentation was confirmed. Compounds from the essential nutrient recipe were tested further to find out the key substrates significantly influencing cider vinegar fermentation.

Genetic and biochemical characterization of genes involved in hyaluronic acid synthesis in Streptococcus zooepidemicus.

The biosynthetic pathway for hyaluronic acid (HA) has been proposed; however, a thorough genetic and functional analysis is required to further elucidate the roles of genes involved in HA production. Previously, we developed a markerless gene-deletion system for Streptococcus zooepidemicus and confirmed that hasA is essential for HA synthesis. Here, we constructed a comprehensive set of deletion mutants and investigated the roles of ten additional predicted genes in the HA synthetic pathway.

[Physiological response to acetic acid stress of Acetobacter pasteuranus during vinegar fermentation].

Objective: The aim of the study is to propose a dynamic acetic acid resistance mechanism through analysis on response of cellular morphology, physiology and metabolism of A. pasteurianus CICIM B7003 during vinegar fermentation.

Methods: Vinegar fermentation was carried out in a Frings 9 L acetator by strain B7003 and cultures were sampled at different cellular growth phases.