3D Film-Like Nanozyme with a Synergistic Amplification Effect for the Ultrasensitive Immunochromatographic Detection of Respiratory Viruses.

Greatly improving the sensitivity and detection range of lateral flow immunoassays (LFAs) by at least 100 times without using additional instruments remains challenging. Herein, we develop a three-dimensional (3D) film-like nanozyme (GO-Pt-AuPt) by ordered assembly of one layer of 30 nm Pt nanoparticles (NPs) and one layer of small Au@Pt satellites (5 nm) onto a two-dimensional (2D) graphene oxide (GO) nanofilm, in which GO greatly increased the interface area and stability of the nanozyme whereas Pt and Au@Pt NPs synergistically enhanced colorimetric/catalytic activities. The grafting of outer Au@Pt satellites converted the 2D nanofilm into a 3D flexible nanozyme with numerous catalytic sites for enzymatic deposition signal amplification and binding sites for target capture.

Identification of chronic non-atrophic gastritis and intestinal metaplasia stages in the Correa's cascade through machine learning analyses of SERS spectral signature of non-invasively-collected human gastric fluid samples.

The progression of gastric cancer involves a complex multi-stage process, with gastroscopy and biopsy being the standard procedures for diagnosing gastric diseases. This study introduces an innovative non-invasive approach to differentiate gastric disease stage using gastric fluid samples through machine-learning-assisted surface-enhanced Raman spectroscopy (SERS). This method effectively identifies different stages of gastric lesions.

Singular perturbation analysis in a coupled Chua's circuit with diffusion.

This paper is concerned with the traveling wave solutions of a singularly perturbed system, which arises from the coupled arrays of Chua's circuit. By the geometric singular perturbation theory and invariant manifold theory, we prove that there exists a heteroclinic cycle consisting of the traveling front and back waves with the same wave speed. In particular, the expression of corresponding wave speed is also obtained.

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance.

Helicobacter pylori is a major human pathogen that infects approximately half of the global population and is becoming a serious health threat due to its increasing antibiotic resistance. It is the causative agent of chronic active gastritis, peptic ulcer disease, and gastric cancer and has been classified as a Group I Carcinogen by the International Agency for Research on Cancer. Therefore, the rapid and accurate diagnosis of H.

Rapid discrimination of spp. and label-free surface enhanced Raman spectroscopy coupled with machine learning algorithms.

and enterotoxigenic (ETEC) are major bacterial pathogens of diarrheal disease that is the second leading cause of childhood mortality globally. Currently, it is well known that spp., and .

Clinical and immunohistochemical analysis of the verrucous and non-verrucous divided nevus of the eyelids.

Purpose: Divided nevus with verrucous hyperplasia will always recur after surgery but non-verrucous divided eyelid nevus rarely recur. This study analyzed the differential expression of Ki-67, S100, Melan A and HMB45 and identified the correlation between the clinical and histopathological features of verrucous and non-verrucous divided eyelid nevus.

Methods: This study included 29 patients, of whom 8 patients had verrucous divided nevus.

Orbital Reconstruction in Le Fort III Fractures.

Background: Few quantitative results are reported about the surgical effect of orbital reconstruction in Le Fort III fractures. The authors' team proposed an ordered surgery method which was effective for Le Fort III fractures. The aim of this study was to evaluate the effectiveness of this method with quantitative outcomes.

Homoclinic orbits in three-dimensional continuous piecewise linear generalized Michelson systems.

In this paper, we investigate the homoclinic orbits for the three-dimensional continuous piecewise linear generalized Michelson systems via analytical methods and numerical simulation. Based on the Poincaré map and invariant manifold theory, we discuss the existence of homoclinic orbits connecting the saddle-focus equilibrium. Finally, numerical simulations are presented to illustrate our results.

Bioresorbable Implants in Reduction of Paediatric Zygomaticomaxillary Complex Fractures Concurrent With Internal Orbital Reconstruction.

Purpose: To evaluate the clinical effectiveness and safety of bioresorbable implants for treating paediatric zygomaticomaxillary complex (ZMC) fractures with concomitant orbital floor defects.

Methods: A retrospective review of paediatric patients who underwent ZMC repair with concomitant orbital floor fractures with bioresorbable implants in Shanghai Ninth People's Hospital from July 2015 to June 2019 was performed. The primary outcome measures included ocular motility, diplopia, enophthalmos, facial deformities, and restricted mouth opening, as well as complication rates.

Myocutaneous sliding flap for reconstruction of divided eyelid nevus.

Objective: To evaluate the myocutaneous sliding flap for restructuring the eyelid of divided nevus, thus optimizing the aesthetic outcome.

Design: A retrospective clinical study.

Participants: Clinical data from patients with a small or medium-sized divided nevus of the eyelids between January 2015 and December 2018 in the Shanghai Ninth People's Hospital were reviewed.

A custom-made conformer wrapped in lower oral mucosa for the correction of severely contracted socket.

Purpose: To investigate the clinical effects of a custom-made conformer wrapped in lower oral mucosa for the correction of severely contracted socket.

Methods: Clinical data and photographs of 32 patients with severely contracted socket were retrospectively analyzed. Among the 32 cases, 21 were males and 11 were females.

Comparative evaluation of BACTEC FX, BacT/ALERT 3D, and BacT/ALERT VIRTUO automated blood culture systems using simulated blood cultures.

Background: To evaluate the performance of BACTEC FX, BacT/ALERT 3D, and VIRTUO systems using simulated blood culture (BC).

Methods: Two experimental designs based on 'with' or 'without' added trough antibiotic concentrations in bottles were implemented.

Results: For the experiment A, A shorter time to detection (TTD) was observed for most of organisms (17/22) in VIRTUO system.

Orbital malformation with dysplastic infraorbital artery in neurofibromatosis type 1.

Purpose: To report a case with neurofibromatosis type 1 presenting as prominent enophthalmos and abnormal infraorbital artery.

Case Description: A 19-year-old man with a family history of neurofibromatosis presented with prominent right enophthalmos. Computed tomography showed orbital dysplasia and enlarged inferior orbital fissure but no plexiform neurofibroma.

Delayed Orbital Apex Syndrome in the Post-Reconstructed Orbit.

Delayed orbital apex syndrome (OAS) is rare during orbital blowout fracture reconstruction. A 30-year-old woman fractured the right orbital floor in a fall and undergone repairing operation 2 weeks later. After severe sneezing on postoperative day 10, she gradually arose vision loss, ophthalmoplegia, ptosis with a dilated and fixed pupil within few hours, then consulted our department and was diagnosed as OAS.

Removal of Orbital Metallic Foreign Bodies With Image-Guided Surgical Navigation.

Purpose: To describe the use of an image-guided 3-dimensional surgical navigation system for the removal of metallic foreign bodies from the human intraorbital region.

Patients And Methods: Between January 2016 and June 2019, 30 patients with metallic foreign bodies in the orbital area underwent image-guided 3-dimensional surgical navigational removal at the authors' center, and their data were retrospectively analyzed. Patients' age, gender, complaints, cause of initial injury, location, interval between injury, and surgery were recorded.

Prevalence of healthcare-associated infections and antimicrobial use in China: Results from the 2018 point prevalence survey in 189 hospitals in Guangdong Province.

Background: Limited data on healthcare-associated infections (HAIs) are available from the developing world, thus a point prevalence survey was conducted to determine the prevalence of HAIs and antimicrobial use in Guangdong Province, China.

Methods: A standardized methodology for point prevalence surveys on HAIs and antimicrobial use has been developed by the Chinese Nosocomial Infection Control and Quality Improvement Center. The prevalence of HAIs, antimicrobial use, and baseline hospital-level variables were collected in 189 hospitals from June 2017 to May 2018.

The Effects of Intraoperative Tarsorrhaphy on Conjunctival Chemosis During Orbital Fracture Repair Surgery.

Purpose: We employed intraoperative tarsorrhaphy depending on the degree of edema to treat moderate conjunctival chemosis during orbital fracture repair surgery.

Methods: This is a retrospective case review of 1367 patients (1384 eyes) who underwent orbital fracture repair surgery by a transconjunctival approach. All cases of moderate conjunctival chemosis during surgery were included and were divided into 2 groups.

Bioresorbable Material in Secondary Orbital Reconstruction Surgery.

Purpose: To validate the potential of bioresorbable implantation in secondary revisional reconstruction after inadequate primary orbital fracture repair, with assessment of pre- and postoperative clinical characteristics and computed tomography image findings.

Methods: A retrospective chart review was conducted on 16 consecutive patients treated for orbital fractures at Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, with inadequate prior surgeries between July 2010 and June 2017; patients who had suffered orbital blowout fractures had undergone primary surgeries elsewhere. Secondary repair of orbital fractures used bioresorbable material following unsatisfactory primary orbital repair.

Overexpression of a MYB Family Gene, , Increases Drought and Salinity Stress Tolerance in Transgenic Rice.

MYB transcription factors have been demonstrated to play key regulatory roles in plant growth, development and abiotic stress response. However, knowledge concerning the involvement of rice genes in salinity and drought stress resistance are largely unknown. In the present study, we cloned and characterized the gene, which was induced by drought and salinity stress.

A Modified Pre-Auricular Approach in the Treatment of Orbital Zygomatic Maxillary Complex Fractures.

Objective: This study aimed to evaluate the safety of a modified pre-aricular approach in the treatment of orbital zygomatic maxillary complex fractures.

Methods: This was a retrospective study of clinical data from 108 adults who were diagnosed with orbital zygomatic maxillary complex fractures and undergo open reduction and internal fixation through a modified pre-auricular approach. Exophthalmometry and limitation of extraocular muscle movement were determined using a Hertel exophthalmometer or synoptophore examination respectively.

Facile Synthesis of Highly Dispersed CoO Nanoparticles on Expanded, Thin Black Phosphorus for a ppb-Level NO Gas Sensor.

Expanded few-layer black phosphorus nanosheets (FL-BP NSs) were functionalized by branched polyethylenimine (PEI) using a simple noncovalent assembly to form air-stable overlayers (BP-PEI), and a CoO@BP-PEI composite was designed and synthesized using a hydrothermal method. The size of the highly dispersed CoO nanoparticles (NPs) on the FL-BP NSs can be controlled. The BP-C5 (190 °C for 5 h) sensor, with 4-6 nm CoO NPs on the FL-BP NSs, exhibited an ultrahigh sensitivity of 8.