A pan-immune panorama of bacterial pneumonia revealed by a large-scale single-cell transcriptome atlas.

Bacterial pneumonia is a significant public health burden, contributing to substantial morbidity, mortality, and healthcare costs. Current therapeutic strategies beyond antibiotics and adjuvant therapies are limited, highlighting the need for a deeper understanding of the disease pathogenesis. Here, we employed single-cell RNA sequencing of 444,146 bronchoalveolar lavage fluid cells (BALFs) from a large cohort of 74 individuals, including 58 patients with mild (n = 22) and severe (n = 36) diseases as well as 16 healthy donors.

Risk Factors for Multidrug Resistance in Patients Infected with Carbapenem-Resistant : A Nomogram.

Purpose: Our aim was to determine the risk factors for multidrug resistance in patients with carbapenem-resistant (CRKP).

Methods: The information of 196 patients with infection was collected. The patients were subsequently assigned to the carbapenem-resistant, multidrug-resistant, and non-multidrug-resistant groups.

A prognosis prediction chromatin regulator signature for patients with severe asthma.

Severe asthma imposes a physical and economic burden on both patients and society. As chromatin regulators (CRs) influence the progression of multiple diseases through epigenetic mechanisms, we aimed to study the role of CRs in patients with severe asthma. Transcriptome data (GSE143303) from 47 patients with severe asthma and 13 healthy participants was downloaded from the Gene Expression Omnibus database.

Mesoscale Analysis of the Effect of Interfacial Transition Zone on the Compressive Damage of Concrete Based on Discrete Element Method.

The coarse aggregate-mortar interface transition zone (ITZ) has a great influence on the mechanical properties of concrete, which cannot be easily studied using laboratory tests in the mesoscale. In this paper, a series of axial compression tests were conducted using the discrete element method (DEM) on concrete specimens for four phases: coarse aggregates, mortars, aggregate-mortar interface transition zones, and voids. The effects of ITZ strength on macroscopic stress and microscopic cracks under different strength reduction factors were investigated through axial compression testing.