Thermal facial image analyses reveal quantitative hallmarks of aging and metabolic diseases.

Although human core body temperature is known to decrease with age, the age dependency of facial temperature and its potential to indicate aging rate or aging-related diseases remains uncertain. Here, we collected thermal facial images of 2,811 Han Chinese individuals 20-90 years old, developed the ThermoFace method to automatically process and analyze images, and then generated thermal age and disease prediction models. The ThermoFace deep learning model for thermal facial age has a mean absolute deviation of about 5 years in cross-validation and 5.

Single-cell senescence identification reveals senescence heterogeneity, trajectory, and modulators.

Cellular senescence underlies many aging-related pathologies, but its heterogeneity poses challenges for studying and targeting senescent cells. We present here a machine learning program senescent cell identification (SenCID), which accurately identifies senescent cells in both bulk and single-cell transcriptome. Trained on 602 samples from 52 senescence transcriptome datasets spanning 30 cell types, SenCID identifies six major senescence identities (SIDs).

Label-free quantitative detection and comparative analysis of lysine acetylation during the different life stages of .

Proteome-wide lysine acetylation has been documented in apicomplexan parasite and . Here, we conducted the first lysine acetylome in unsporulated oocysts (USO), sporulated 7 h oocysts (SO 7h), sporulated oocysts (SO), sporozoites (S), and the second generation merozoites (SMG) of through a 4D label-free quantitative technique. Altogether, 8532 lysine acetylation sites on 2325 proteins were identified in , among which 5445 sites on 1493 proteins were quantified.

Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance -specific immunity.

The epidemic of avian coccidiosis seriously threatens the animals' welfare and the economic gains of the poultry industry. Widespread in avian coccidiosis, () could obviously impair the production performance of the infected chickens. So far, few effective vaccines targeting have been reported, and the nanovaccines composed of nanospheres captured our particular attention.

Molecular Docking and In Silico Simulation of Membrane-Associated Progesterone Receptor Component 2 () and Its Interaction with Human PGRMC1.

. is a foodborne zoonotic disease caused by spp., including .

Molecular Characterization of spp. among Dairy, Cashmere, and Meat Goats in Shaanxi Province, Northwestern China.

Anaplasma spp. are important tick-borne pathogens endangering the health of humans and various animals. Although several studies have reported Anaplasma infection in livestock in China, little is known about the impact of production categories on the occurrence of Anaplasma species.

PLGA Nanospheres as Delivery Platforms for 1a Protein: A Novel Strategy to Improve Specific Immunity.

The infections of chicken coccidiosis impact the welfare of chickens and the economical production of poultry. is ubiquitous in chicken coccidiosis, and infection can significantly affect the productivity of birds. Up to now, few efficient vaccines against have been reported, whereas the recombinant subunit vaccines delivered by nanomaterials may elicit an encouraging outcome.

A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens.

With a worldwide distribution, Eimeria spp. could result in serious economic losses to the poultry industry. Due to drug resistance and residues, there are no ideal drugs and vaccines against Eimeria spp.

Nano vaccines for Ribosomal P2 Protein With Nanomaterials as a Promising DNA Vaccine Against Toxoplasmosis.

Caused by , toxoplasmosis has aroused great threats to public health around the world. So far, no effective vaccine or drug is commercially available, and the demands for a safe and effective therapeutic strategy have become more and more urgent. In the current study, we constructed a DNA vaccine encoding ribosomal P2 protein (TgP2) and denoted as TgP2-pVAX1 plasmid.

Nano DNA Vaccine Encoding Histone Deacetylase SIR2 Enhanced Protective Immunity in Mice.

The pathogen of toxoplasmosis, (), is a zoonotic protozoon that can affect the health of warm-blooded animals including humans. Up to now, an effective vaccine with completely protection is still inaccessible. In this study, the DNA vaccine encoding histone deacetylase SIR2 (pVAX1-SIR2) was constructed.

With Chitosan and PLGA as the Delivery Vehicle, Oxidoreductase-Based DNA Vaccines Decrease Parasite Burdens in Mice.

() is an intracellular parasitic protozoan that can cause serious public health problems. However, there is no effectively preventive or therapeutic strategy available for human and animals. In the present study, we developed a DNA vaccine encoding oxidoreductase from short-chain dehydrogenase/reductase family (TgSDRO-pVAX1) and then entrapped in chitosan and poly lactic-co-glycolic acid (PLGA) to improve the efficacy.

Characterization of Membrane-Associated Progesterone Receptor Component-2 from and Its Interaction with Progesterone and Mifepristone.

is a foodborne zoonotic disease caused by spp., including . In the present study, membrane-associated progesterone receptor component-2 () gene was cloned and characterized using protein sequencing analysis.

Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant.

As an important zoonotic protozoan, () has spread around the world, leading to infections in one-third of the population. There is still no effective vaccine or medicine against , and recombinant antigens entrapped within nanospheres have benefits over traditional vaccines. In the present study, we first expressed and purified proteasome subunit alpha type 1 (TgPSA1), then encapsulated the recombinant TgPSA1 (rTgPSA1) in chitosan nanospheres (CS nanospheres, rTgPSA1/CS nanospheres) and incomplete Freund's adjuvant (IFA, rTgPSA1/IFA emulsion).

Assessing the rate of aging to monitor aging itself.

Healthy aging is the prime goal of aging research and interventions. Healthy aging or not can be quantified by biological aging rates estimated by aging clocks. Generation and accumulation of large scale high-dimensional biological data together with maturation of artificial intelligence among other machine learning techniques, have enabled and spurred the rapid development of various aging rate estimators (aging clocks).

Recombinant Ribosomal Protein P2 Modulates the Functions of Murine Macrophages In Vitro and Provides Immunity against Acute Toxoplasmosis In Vivo.

Almost every warm-blooded animal can be an intermediate host for (); there is still no efficient vaccine and medicine available for infections. Detected on the surface of free tachyzoites of , ribosomal protein P2 (TgRPP2) has been identified as a target for protection against toxoplasmosis. In the present study, TgRPP2 was firstly expressed in a prokaryotic expression system, and the purified recombinant TgRPP2 (rTgRPP2) was characterized by its modulation effects on murine macrophages.

Histone deacetylase SIR2 in Toxoplasma gondii modulates functions of murine macrophages in vitro and protects mice against acute toxoplasmosis in vivo.

Silent information regulator 2 (SIR2) in histone deacetylase (HDAC) is particularly conserved and widely expressed in all eukaryotic cells. HDAC is a crucial post-translational modification protein regulating gene expression. In the present study, a Toxoplasma gondii (T.

Modulation Effects of Histone H2A1 on Murine Macrophages and Encapsulation with Polymer as a Vaccine Candidate.

() is the most common zoonotic protozoa and has infected about one-third of the population worldwide. Recombinant epitopes encapsulated in nanospheres have advantages over traditional vaccines. For an efficient delivery system, poly (DL-lactide-co-glycolide) (PLGA) and chitosan are the most frequently used biodegradable polymeric nanospheres with strong safety profiles.

Individual variation of the SARS-CoV-2 receptor ACE2 gene expression and regulation.

The COVID-19 coronavirus is now spreading worldwide. Its pathogen, SARS-CoV-2, has been shown to use angiotensin-converting enzyme 2 (ACE2) as its host cell receptor, same as the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003. Epidemiology studies found males although only slightly more likely to be infected than females account for the majority of the severely ill and fatality, which also bias for people older than 60 years or with metabolic and cardiovascular diseases.

Increased Progastrin-Releasing Peptide Expression is Associated with Progression in Gastric Cancer Patients.

Purpose: The purpose of this study was to assess the diagnostic and prognostic value of serum progastrin-releasing peptide (ProGRP) in patients with gastric cancer (GC).

Materials And Methods: A total of 150 patients with GC (89 males and 61 females) were recruited, including those with stage I (n=28), stage II (n=33), stage III (n=50), and stage IV (n=39) disease; 50 healthy controls and 66 patients with benign gastric diseases were also enrolled. Levels of serum ProGRP, carcinoembryonic antigen (CEA), and carbohydrate antigen 72-4 (CA72-4) were measured in all subjects.

MOLECULAR CHARACTERIZATION OF THEILERIA SPP. IN GOATS FROM SHAANXI PROVINCE, NORTHWESTERN CHINA.

Theileriosis is an important tick-borne pathogen of livestock globally, causing severe reduction of livestock productivity and economic loss. To systematically investigate the prevalence and species of Theileria spp. in goats from Shaanxi province, a total of 509 blood samples were collected from dairy, cashmere and meat goats from 7 counties, and examined by using the microscopic examination and the nested PCR targeting the SSU rRNA gene.

The association between interleukin-19 concentration and diabetic nephropathy.

Background: Interleukin-19 (IL-19) is a newly discovered cytokine belonging to the Interleukin-10(IL-10) family. IL-19 have indispensable functions in many inflammatory processes and also can induce the angiogenic potential of endothelial cells. The purpose of present study was to investigate the relation of serum interleukin-19 (IL-19) levels with diabetic nephropathy (DN).

Association between interleukin-19 and angiopoietin-2 with vascular complications in type 2 diabetes.

Aims/introduction: The aim of the present study was to investigate the role of interleukin-19 (IL-19) in angiogenesis of type 2 diabetes mellitus with complications, and to assess the relationship of serum IL-19 and angiopoietin-2 (Ang-2) in type 2 diabetes mellitus.

Materials And Methods: The group studied comprised of 240 patients with type 2 diabetes mellitus (132 men and 108 women), and included macrovascular complications, microvascular complications and type 2 diabetes mellitus without vascular complications. The control group consisted of 50 healthy blood donors.