Effective Modulation of Ion Mobility through Solid-State Single-Digit Nanopores.

Many experimental studies have proved that ion dynamics in a single-digit nanopore with dimensions comparable to the Debye length deviate from the bulk values, but we still have critical knowledge gaps in our understanding of ion transport in nanoconfinement. For many energy devices and sensor designs of nanoporous materials, ion mobility is a key parameter for the performance of nanofluidic equipment. However, investigating ion mobility remains an experimental challenge.

Large-Scale Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities.

Over the past decades, remarkable progress on phosphoramidite chemistry-based large-scale oligonucleotide synthesis has been achieved, enabling numerous novel and exciting applications. Among them, genome synthesis and DNA data storage are striking. However, to make these two applications more practical, the synthesis length, speed, cost, and throughput require vast improvements, which is a challenge to be met by the phosphoramidite chemistry.

Inhibition of Glycogen Synthase Kinase 3β Increases the Proportion and Suppressive Function of CD19CD24CD27 Breg Cells.

CD19CD24CD27 memory Breg cells exhibit decreased abundance in patients with chronic graft-versus-host disease (cGVHD) after liver transplantation and produce less IL-10 than those from patients without cGVHD and healthy donors. Due to the lack of Breg cells and the difficulty in expanding them , in mouse models and early human clinical trials, the adoptive transfer of Breg cells to autoimmune diseases is greatly restricted. Glycogen synthase kinase 3β (GSK-3β) is a multifunctional serine/threonine (ser/thr) protein kinase that can participate in B cell growth, metabolic activity, and proliferation.

[Gene Mutants and Their Clinical Characteristics of G6PD Deficiency Among Children in Luzhou Area].

Objective: To study the gene mutants of G6PD deficiency and their clinical featuers among children in Luzhou area.

Methods: 732 children with suspected G6PD deficiency in Luzhou area from March 2017 to July 2019 were selected, which were examined for G6PD enzyme activity and gene mutation. The G6PD enzyme activity was detected by ultraviolet rate quantification, and the gene mutation was detected by melting curve analysis-based PCR assay, and the clinical characteristics of different mutants when acute hemolysis happens were analyzed.

Noble scallop, Chlamys nobilis, sperm motility duration in the post-activation phase.

Sperm motility in the post-activation phase is important for conducting and assessing species-specific artificial fertilization protocols. This study characterizes spermatozoa movement of the noble scallop, Chlamys nobilis, during the post-activation phase. Sperm samples were diluted and activated by fresh seawater, and subsequently incubated at 26 °C for 4 h.

Polyelectrolyte Binder for Sulfur Cathode To Improve the Cycle Performance and Discharge Property of Lithium-Sulfur Battery.

To achieve the higher capacity and the better cycle performance of the lithium-sulfur (L-S) batteries, a copolymer electrolyte prepared via emulsifier-free emulsion polymerization was used as the binder for the sulfur cathode in this study. This polyelectrolyte binder has uniform dispersion and good Li conductivity in the cathode that can improve the kinetics of sulfur electrochemical reactions. As a result, the capacity and cycle performance of the battery are improved evidently when the cell is discharged to 1.

A deep study of the protection of Lithium Cobalt Oxide with polymer surface modification at 4.5 V high voltage.

Charging the cells above a conventional voltage of 4.2 V is a promising attempt to increase the energy density of Lithium Cobalt Oxide (LCO), however, the problem of crystal instability at high voltage that leading deterioration of cycle performance needs to be urgently resolved. In this work, as an effective and easy approach to improve the cycle performance and crystal stability of LCO cycling at 4.

Application of protein typing in molecular epidemiological investigation of nosocomial infection outbreak of aminoglycoside-resistant Pseudomonas aeruginosa.

Pseudomonas aeruginosan has emerged as an important pathogen elated to serious infections and nosocomial outbreaks worldwide. This study was conducted to understand the prevalence of aminoglycoside (AMG)-resistant P. aeruginosa in our hospital and to provide a scientific basis for control measures against nosocomial infections.

An enzyme free electrochemical biosensor for sensitive detection of miRNA with a high discrimination factor by coupling the strand displacement reaction and catalytic hairpin assembly recycling.

An isothermal, enzyme free, ultra-specific and ultra-sensitive protocol for electrochemical detection of miRNAs is proposed based on the toehold-mediated strand displacement reaction (SDR) and non-enzymatic catalytic hairpin reaction (CHA) recycling. The SDR was first triggered only in the presence of target miRNA and this process also affects other miRNA interferences having similar target sequences, thus guaranteeing a high discrimination factor and could be used in rare content miRNA detection with various amounts of interferences having similar target sequences. The output protector strand then triggered enzyme free CHA amplification and generates plenty of hairpin self-assembly products.

Circulating tumor DNA is effective for detection of KRAS mutation in colorectal cancer: a meta-analysis.

Background:: Circulating tumor DNA (ctDNA) offers a novel and minimally invasive approach to the detection of the KRAS oncogene mutation in colorectal cancer. This study was conducted to compare the prognostic value of ctDNA with that of the current gold standard tumor tissue analysis.

Methods:: A systematic literature review was conducted to identify relevant articles published from inception to December 27, 2016; the PubMed, Web of Science, Embase, Wanfang and China National Knowledge Infrastructure databases were searched.

Molecular characterization and expression analysis of chitinase from the pearl oyster Pinctada fucata.

Chitinase is an enzyme that plays an important role in the chitin metabolism of a wide range of organisms. However, the function of chitinase in the pearl oyster Pinctada fucata is yet to be determined. In this study, a chitinase gene (named PfChi1) was cloned from P.

Platelet proteomics in diagnostic differentiation of primary immune thrombocytopenia using SELDI-TOF-MS.

Background: Primary immune thrombocytopenic purpura (pITP) is defined as isolated autoimmune thrombocytopenia with idiopathic low platelet count, normal bone marrow, and unexplained causes of thrombocytopenia. Currently there is no definite criterion for ITP diagnosis.

Methods: We conducted proteomic screen of patients with pITP, secondary immune thrombocytopenia (sITP), and healthy controls using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).

MicroRNAs in body fluids as biomarkers for non-small cell lung cancer: a systematic review.

Non-small cell lung cancer (NSCLC) is one of the most common life-threatening malignant tumors. A test for early diagnosis of NSCLC needs to be not too invasive and not too heavy a burden for weakened patients. A series of studies reported various microRNAs (miRNAs) could be novel serum biomarkers for NSCLC.

Kinetic analysis of one-step solid-state reaction for Li4Ti5O12/C.

The kinetics of one-step solid-state reaction of Li(4)Ti(5)O(12)/C in a dynamic nitrogen atmosphere was first studied by means of thermogravimetric-differential thermal analysis technique at five different heating rates. According to the double equal-double steps method, the Li(4)Ti(5)O(12)/C solid-state reaction mechanism could be properly described as the Jander equation, which was a three-dimensional diffusion with spherical symmetry, and the reaction mechanism functions were listed as follows: f(α) = (3)/(2)(1 - α)(2/3)[1 - (1 - α)(1/3)](-1), G(α) = [1 - (1 - α)(1/3)](2). In FWO method, average activation energy, frequency factor, and reaction order were 284.

[Detection of fungi in liquor workers with tinea corporis and tinea cruris using arbitrarily primed polymerase chain reaction].

Objective: To explore the method of rapid detection of skin fungi and the significance of conventional diagnosis liquor worker tinea corporis and tinea cruris using arbitrarily primed polymerase chain reaction AP-PCR.

Methods: Among liquor workers who were 50 tinea corporis patients, 58 tinea cruris patients and 50 health persons, we amplified the DNAs of the dermatophytes were amplified using AP-PCR and random primers OPD18 5'-GAGAGCCAAC-3' and OPAA11 5'-ACCCGACCTG-3', at the same time, the dermatophytes with microscope were detected and cultured.

Results: AP-PCR analysis detected fungal DNA in 45 patients(90.

Effect of human cytomegalovirus on proliferation of hematopoietic progenitor cells of cord blood.

Objective: This study was designed to investigate the effect of human cytomegalovirus (HCMV) on the proliferation of colony forming unit granulocyte-macrophage (CFU-GM), CFU-erythroid (CFU-E), burst forming unit-erythroid (BFU-E), CFU-multipotential (CFU-Mix) and CFU-megakaryocytic (CFU-Mk) progenitor cells of cord blood in vitro as well as the possible mechanism.

Methods: Twenty cord blood specimens were collected from the umbilical vein of normal full-term neonates delivered spontaneously. This study consisted of five groups: 3 Infection groups in which 0.

Epidemiology of assaultive injuries in areas of Sichuan province, China.

Objective: To scrutinize the epidemiological characteristics of assaultive injuries in Sichuan province, China.

Methods: A survey of all cases of assaultive injuries reported by police was performed during 8 years in eight counties of Sichuan province, China. A total of 2862 victims and 2856 offenders were registered.

[Influence of ganciclovir and astragalus membranaceus on proliferation of hematopoietic progenitor cells of cord blood after cytomegalovirus infection in vitro].

Objective: Cytomegalovirus (CMV) infection was greatly common in the world. CMV infection produces usually mild or asymptomatic infections in individuals with normal immune responses, whereas it may cause serious disease in immunosuppressive patients. Clinical manifestations include suppression of myelopoiesis, a mononucleosis like syndrome, hepatosplenomegaly, lymphadenopathy, thrombocytopenia, and hemolytic anemia.