Associations between the indoor microbiome, environmental characteristics and respiratory infections in junior high school students of Johor Bahru, Malaysia.

Pathogens are commonly present in the human respiratory tract, but symptoms are varied among individuals. The interactions between pathogens, commensal microorganisms and host immune systems are important in shaping the susceptibility, development and severity of respiratory diseases. Compared to the extensive studies on the human microbiota, few studies reported the association between indoor microbiome exposure and respiratory infections.

Indoor microbiome, environmental characteristics and asthma among junior high school students in Johor Bahru, Malaysia.

Indoor microbial diversity and composition are suggested to affect the prevalence and severity of asthma by previous home microbiome studies, but no microbiome-health association study has been conducted in a school environment, especially in tropical countries. In this study, we collected floor dust and environmental characteristics from 21 classrooms, and health data related to asthma symptoms from 309 students, in junior high schools in Johor Bahru, Malaysia. The bacterial and fungal composition was characterized by sequencing 16s rRNA gene and internal transcribed spacer (ITS) region, and the absolute microbial concentration was quantified by qPCR.

[Identification of the scope of practice for dental nurses with Delphi method].

Purpose: To identify the practice scope of dental nurses under the new situations.

Methods: The draft of scope of practice for dental nurses was based on theoretical analysis, literature review and consultation of advisory panel, and the final scope of practice for dental nurses was established by using the Delphi method. Statistical analysis was implemented using coefficient of variation, Kendall W with SPSS 17.

High correlation of specific IgE sensitization between birch pollen, soy and apple allergens indicates pollen-food allergy syndrome among birch pollen allergic patients in northern China.

Background: Birch pollen sensitization and associated pollen-food syndrome among Chinese allergic patients have not been investigated.

Methods: Sera from 203 allergic patients from the northern part of China and collected during February to July 2014 were investigated. Specific immunoglobulin E (IgE) against birch pollen extract Bet v and major birch pollen allergen Bet v 1 were measured using the ADVIA Centaur.

[Optimal sequence algorithm of divided period appointment consultation service sequence for re-visiting patients in the department of stomatology].

Purpose: An optimal sequence algorithm model of divided period appointment consultation service was built through optimal sequence algorithm.

Methods: Re-visiting patients in the department of stomatology in a tertiary hospital were enrolled as the research objects in this study. Information about the diagnose term of common diseases, tooth positions treated, doctor's consultation hours were collected.

Indoor Allergen Levels and Household Distributions in Nine Cities Across China.

Objective: Chinese allergic subjects have high levels of sensitization to house dust mite (HDM) and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households.

Methods: Dust samples were collected from nine cities.

[Optimize visiting sequence and time-interval appointment of re-visiting patients in stomatology].

Purpose: To optimize visiting sequence and time-interval appointment mode to reduce waiting time of re-visiting patients in department of stomatology based on clinical data repository.

Methods: Waiting time data of re-visiting patients in department of stomatology was collected and divided into 2 groups. A total of 70897 patients from July 2014 to September 2014 were enrolled as experimental group (group year-2014), while 63960 patients from July 2013 to September 2013 were used as control group (group year-2013).

Endotoxin, ergosterol, fungal DNA and allergens in dust from schools in Johor Bahru, Malaysia- associations with asthma and respiratory infections in pupils.

There are few studies on associations between respiratory health and allergens, fungal and bacterial compounds in schools in tropical countries. The aim was to study associations between respiratory symptoms in pupils and ethnicity, chemical microbial markers, allergens and fungal DNA in settled dust in schools in Malaysia. Totally 462 pupils (96%) from 8 randomly selected secondary schools in Johor Bahru, Malaysia, participated.

[Optimize the service flow of stomatological department to accommodate outpatient's demands].

Purpose: To provide measures to dispose of unsatisfactory service links for outpatients, and to optimize the service flow of department of stomatology.

Methods: Four hundred and seventy-nine questionnaires were delivered in Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. The questionnaires included 15 questions relating to general experience in department of stomatology and assessment on separate link of service flow.

Prevalence of sensitization to weed pollens of Humulus scandens, Artemisia vulgaris, and Ambrosia artemisiifolia in northern China.

Objective: Weed pollens are common sources of allergens worldwide. The prevalence of weed pollen sensitization is not yet fully known in China. The purpose of this study was to investigate the prevalence of sensitization to weed allergens from Artemisia, Ambrosia, and Humulus in northern China.

Indoor mite allergen levels, specific IgE prevalence and IgE cross-inhibition pattern among asthmatic children in Haikou, southern China.

Background: Haikou locates in tropical island with unique mite propagation. The aim of this stuy is to determine mite allergens levels in Haikou, and to investigate the prevalence of mite specific IgE-sensitization and IgE cross-reactivity between house dust mites.

Methods: Allergen and antigen concentrations against six mite species were tested by enzyme-linked immunosorbent assay (ELISA).

Allergen micro-array detection of specific IgE-reactivity in Chinese allergy patients.

Background: Allergen micro-arrays are powerful tools for screening of serum IgE-reactivity. In this study allergen micro-arrays were used to identify dominating IgE-binding allergens and cross-reactivity patterns among selected Chinese allergy patients.

Methods: The study was conducted using patient sera from the cities of Guangzhou, Nanjing, Chengdu and Shenyang.

[A study of Rutter behavior problems in school aged children with cleft lip and/or palate].

Purpose: To study the difference of behavior problems in school aged children with cleft of lip and/or palate.

Methods: The Rutter Children Behavior Parent Checklist was used in 100 school aged children with cleft lip and/or palate and 135 school aged normal children in Shanghai.The questionnaire were filled and analyzed with chi2 test and logistic regression using SPSS 10.

Optimal calibration method for water distribution water quality model.

A water quality model is to predict water quality transport and fate throughout a water distribution system. The model is not only a promising alternative for analyzing disinfectant residuals in a cost-effective manner, but also a means of providing enormous engineering insights into the characteristics of water quality variation and constituent reactions. However, a water quality model is a reliable tool only if it predicts what a real system behaves.