MdNAC5: a key regulator of fructose accumulation in apple fruit.

The sweetness of apple fruit is a key factor in the improvement of apple varieties, with fructose being the sweetest of the soluble sugars, playing a crucial role in determining the overall sweetness of the apple. Therefore, uncovering the key genes controlling fructose accumulation and deciphering the regulatory mechanisms of fructose are vitally important for the improvement of apple varieties. In this study, through BSA-seq and transcriptome analysis of the 'Changfu 2' × 'Golden Delicious' F1 hybrid population, MdNAC5 was identified as a key regulatory gene for fructose content.

Plasma Membrane-Localized Transporter MdSWEET12 Is Involved in Sucrose Unloading in Apple Fruit.

Sugar content is an important factor determining the flavor in apple fruit. Sugar unloading is a prerequisite step for sugar accumulation. However, little is known about sugar unloading mechanisms in apple.

The MdBBX22-miR858-MdMYB9/11/12 module regulates proanthocyanidin biosynthesis in apple peel.

Proanthocyanidins (PAs) have antioxidant properties and are beneficial to human health. The fruit of apple (Malus × domestica Borkh.), especially the peel, is rich in various flavonoids, such as PAs, and thus is an important source of dietary antioxidants.

DWPPI: A Deep Learning Approach for Predicting Protein-Protein Interactions in Plants Based on Multi-Source Information With a Large-Scale Biological Network.

The prediction of protein-protein interactions (PPIs) in plants is vital for probing the cell function. Although multiple high-throughput approaches in the biological domain have been developed to identify PPIs, with the increasing complexity of PPI network, these methods fall into laborious and time-consuming situations. Thus, it is essential to develop an effective and feasible computational method for the prediction of PPIs in plants.

MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel.

The red coloration of apple ( × Borkh.) is due to the accumulation of anthocyanins in the fruit peel. Light is essential for anthocyanin biosynthesis in apple.

Improved prediction of drug-target interactions based on ensemble learning with fuzzy local ternary pattern.

: The prediction of interacting drug-target pairs plays an essential role in the field of drug repurposing, and drug discovery. Although biotechnology and chemical technology have made extraordinary progress, the process of dose-response experiments and clinical trials is still extremely complex, laborious, and costly. As a result, a robust computer-aided model is of an urgent need to predict drug-target interactions (DTIs).

An Ensemble Learning-Based Method for Inferring Drug-Target Interactions Combining Protein Sequences and Drug Fingerprints.

Identifying the interactions of the drug-target is central to the cognate areas including drug discovery and drug reposition. Although the high-throughput biotechnologies have made tremendous progress, the indispensable clinical trials remain to be expensive, laborious, and intricate. Therefore, a convenient and reliable computer-aided method has become the focus on inferring drug-target interactions (DTIs).

Metabolomic insights into the browning of the peel of bagging 'Rui Xue' apple fruit.

Background: Bagging is one of the most important techniques for producting high-quality fruits. In the actual of cultivating, we found a new kind of browning in peel of apple fruit that occurs before harvest and worsen during storage period. There are many studies on metabonomic analysis of browning about storage fruits, but few studies on the mechanism of browning before harvest.

mdm-miR828 Participates in the Feedback Loop to Regulate Anthocyanin Accumulation in Apple Peel.

Anthocyanins are responsible for the red pigmentation in the peel of apple ( Borkh.) fruit. Relatively few studies have investigated anthocyanins at the posttranscriptional level.

[Studies on resistance of to praziquantel XVII Biological characteristics of praziquantel-resistant isolates of in mice].

Objective: To investigate the biological characteristics of the praziquantel-resistant isolate of in mice, so as to explore the pathogenicity to definitive hosts and transmission intensity of the praziquantel-resistant isolate of .

Methods: Mice were infected with the cercariae released from two praziquantel-resistant isolates and two praziquantel-susceptible isolates of . The mouse- snail-mouse cycle was established and maintained in the laboratory.

[Studies on resistance of to praziquantel XⅥ Biological characteristics of praziquantel-resistant isolates of in snails].

Objective: To investigate the difference of biological characteristics between the praziquantel-resistant and -susceptible isolates of in intermediate host snails.

Methods: Mice were infected with cercariae of praziquantel-resistant and -susceptible isolates of , and the parasite eggs were collected 37 days post-infection to hatch miracidium. Then, the snails were infected with the miracidium of each parasite isolate.

[Surveillance and forecast system of schistosomiasis in Jiangsu Province. VI. Detection technology of water infectivity based on enrichment of Schistosoma japonicum cercariae on water surface].

Objective: To explore the enrichment technique of Schistosoma japonicum cercariae on the water surface, so as to establish a new method combined with the existing technology to detect the cercarial infested water body quickly and sensitively.

Methods: Soybean oil, gasoline, kerosene and isophorone were screened as expanding agents. The cercariae were enriched by the thrust of the expanding agents when diffusing on the water surface, and PE adsorption film and C-6 film were applied to seize them so as to determine the infectivity of the water quickly.