[Cloning of human PAK6 cDNA, preparation of anti-PAK6 polyclonal antibody and PAK6 expression in prostate cancer].

Objective: To study the role of PAK6 in prostate cancer by cloning PAK6-N terminal sequence into E.coli and preparing its polyclonal rabbit antibody to detect PAK6 expression in prostate cancer.

Methods: Based on human PAK6 cDNA sequence, we designed a pair of primers to amplify the PAK6-N terminal sequence by PCR.

[Preparation of anti-P21-activated kinase 5 polyclonal antibody and its application in dental germ cells].

Objective: To clone PAK5-N terminal sequence for expression in E. coli to prepare its polyclonal antibody, and examine the role of PAK5 in dental germ cells.

Methods: Based on human PAK5 cDNA sequence, PCR primers were designed to amplify PAK5-N terminal sequence.