Protein nanoparticles as potent delivery vehicles for polycytosine RNA-binding protein one.

Ma recently reported in the that ferroptosis occurs in osteoblasts under high glucose conditions, reflecting diabetes pathology. This condition could be protected by the upregulation of the gene encoding polycytosine RNA-binding protein 1 (PCBP1). Additionally, Ma used a lentivirus infection system to express PCBP1.

Targeting nuclear factor erythroid 2-related factor 2-regulated ferroptosis to treat nervous system diseases.

By critically examining the work, we conducted a comprehensive bibliometric analysis on the role of nuclear factor erythroid 2-related factor 2 (NRF2) in nervous system diseases. We also proposed suggestions for future bibliometric studies, including the integration of multiple websites, analytical tools, and analytical approaches, The findings presented provide compelling evidence that ferroptosis is closely associated with the therapeutic challenges of nervous system diseases. Targeted modulation of NRF2 to regulate ferroptosis holds substantial potential for effectively treating these diseases.

Encapsulating taurine into liposomes: A promising therapeutic for liver fibrosis.

We summarize the mechanism by which taurine (Tau) inhibits autophagy and induces iron apoptosis in hepatic stellate cells. Tau interacts with autophagy regulates multifunctional proteins, microtubule-associated protein 1 light chain 3 Beta, and autophagy-related gene 5 to inhibit autophagy, binds to ferritin heavy chain 1 and nuclear receptor coactivator 4 to trigger ferritin autophagy, and interacts with glutathione peroxidase 4 to promote iron apoptosis. There is a solid rationale for developing Tau-based therapies targeting autophagy and ferroptosis regulation.

Research on nanosciences involvement in pharmaceutical education should be reinforced.

Inclusion of nanoscience in pharmaceutical education should be reinforced, in order to match the demand of current pharmaceutical talent cultivation.

[Effect of bleaching combined with Er:YAG or Nd:YAG laser on shear bond strength and microleakage of resin restoration].

Purpose: To evaluate the effects of bleaching combined with Er:YAG laser or Nd:YAG laser on bond strength and microleakage of resin fillings on enamel surface.

Methods: Sixty-four pieces of enamel specimens prepared from isolated teeth were randomly divided into 4 groups(n=16): control group, simple bleaching group, bleaching combined with Er: YAG laser group and bleaching combined with Nd:YAG laser group. Then the shear bond strength and the depth of microleakage were tested, and the fracture mode of the specimen was observed under microscope.

Ecological shifts of salivary microbiota associated with metabolic-associated fatty liver disease.

Introduction: Metabolic-associated fatty liver disease (MAFLD) is the most common chronic liver disease related to metabolic syndrome. However, ecological shifts in the saliva microbiome in patients with MAFLD remain unknown. This study aimed to investigate the changes to the salivary microbial community in patients with MAFLD and explore the potential function of microbiota.

[Correlation analysis of supragingival microbiome and host blood lipid levels].

Purpose: To analyze the correlation between microbiome of supragingival plaque and host blood lipid levels.

Methods: Samples of supragingival plaques from 68 volunteers aged 45-60 years were collected. The total DNA was extracted, and 16S rDNA V3-V4 regions were amplified via PCR.

[Correlational Study of Thyroid-Stimulating Hormone and Salivary Microecology].

Objective: To investigate the relationship between the composition of salivary microecology and thyroid-stimulating hormone (TSH) levels in healthy adults.

Methods: Healthy subjects were included in the high-TSH group (=22, 3.00-4.

[Evaluation on the fracture resistance of dental tissues after guided plate-mediated precision minimally invasive root canal treatment].

Purpose: In order to achieve accurate and minimally invasive root canal treatment and enhance the fracture resistance of tooth tissue after root canal therapy, this study explores digital guided mediated minimally invasive root canal treatment and compares it with conventional root canal treatment to provide a more favorable method for clinical practice.

Methods: Forty freshly extracted first permanent molars were randomly divided into control group and experimental group. Teeth in the control group were treated with conventional root canal treatment, while teeth in the experimental group were treated with precise minimally invasive root canal treatment.

Shifts in the Bacterial Community of Supragingival Plaque Associated With Metabolic-Associated Fatty Liver Disease.

Metabolic-associated fatty liver disease (MAFLD), also known as the hepatic manifestation of metabolic disorders, has become one of the most common chronic liver diseases worldwide. The associations between some oral resident microbes and MAFLD have been described. However, changes to the oral microbial community in patients with MAFLD remain unknown.

[Shanghai municipal expert consensus on standardized prevention and control of COVID-19 during procedures of oral radiology].

The announcement of National Health Commission on January 20, 2020 (No.1 of 2020) has included novel coronavirus pneumonia into the B class infectious diseases according to the law of the People's Republic of China on the prevention and control of infectious diseases, and has been managed as A class infectious diseases. People's governments at all levels and health administration departments have been paying high attention to it.

[In vitro study of apical negative pressure irrigation on apical extrusion and E. faecalis elimination].

Purpose: To compare the efficiency of negative pressure irrigation and conventional syringe irrigation and evaluate the possibility as a new way of irrigation.

Methods: In vitro silicone socket model was used to simulate the resistance of apical flow by apical surrounding tissues. The in vitro efficacy on apical extrusion and E.

[The effect of Streptococcus mutans luxS gene on mixed-species biofilm communities].

Purpose: To evaluate the effect of S.mutans luxS gene on mixed-species biofilms communities.

Methods: Biofilms were formed by S.

p300 and C/EBPβ-regulated IKKβ expression are involved in thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells.

Asthma and chronic obstructive pulmonary disease (COPD) are common chronic lung inflammatory diseases. Thrombin and interleukin (IL)-8/C-X-C chemokine ligand 8 (CXCL8) play critical roles in lung inflammation. Our previous study showed that c-Src-dependent IκB kinase (IKK)/IκBα/nuclear factor (NF)-κB and mitogen-activated protein kinase kinase kinase 1 (MEKK1)/extracellular signal-regulated kinase (ERK)/ribosomal S6 protein kinase (RSK)-dependent CAAT/enhancer-binding protein β (C/EBPβ) activation are involved in thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells.

[Construction of luxS gene knockout mutant of Enterococcus faecalis].

Puppose: To construct quorum sensing luxS knockout mutants of Enterococcus faecalis through homologous recombination.

Methods: The upstream and downstream flank DNA fragments of E. faecalis luxS gene (up, dn) and erythromycin resistance gene (erm) were amplified by PCR.

[Exploring the stem cell surface markers expressed in human dental pulp stem cells].

Purpose: To explore the stem cell surface markers expressed in human dental pulp stem cells which were selected and isolated by magnetic beads.

Methods: Human dental pulp cells (hDPCs) were separated and cultured from dental pulp of healthy third molars for orthodontic purpose. HDPSCs were isolated from cultured hDPCs by magnetic-activated cell sorting's (MACS) indirect magnetic cell labeling and positive selection strategy with antibody STRO-1 in the 2nd generation.

The impact of various time intervals on the supragingival plaque dynamic core microbiome.

Objective: The aim of this study was to examine the influence of various time intervals on the composition of the supragingival plaque microbiome, especially the dynamic core microbiome, and to find a suitable observation interval for further studies on oral microbiota.

Methods And Materials: Eight qualified volunteers whose respective age ranges from 25 to 28 years participated in the present study. The supragingival plaque was collected from the buccogingival surface of the maxillary first molar at eight time slots with different intervals (day 0, 1 day, 3 days, 1 week, 2 weeks, 3 weeks, 1 month, and 3 months).

[Complementation and function of methyl-metabolic pathway in Streptococcus mutans luxS null strain].

Purpose: To complement the activated methyl cycle (AMC) pathway at an AI-2 defect background in Streptococcus mutans (S. mutans) luxS null strain.

Methods: A sahH gene was amplified from Pseudomonas aeruginosa and introduced into the S.

[The net analyte preprocessing combined with radial basis partial least squares regression applied in noninvasive measurement of blood glucose].

In order to improve the prediction accuracy of quantitative analysis model in the near-infrared spectroscopy of blood glucose, this paper, by combining net analyte preprocessing (NAP) algorithm and radial basis functions partial least squares (RBFPLS) regression, builds a nonlinear model building method which is suitable for glucose measurement of human, named as NAP-RBFPLS. First, NAP is used to pre-process the near-infrared spectroscopy of blood glucose, in order to effectively extract the information which only relates to glucose signal from the original near-infrared spectra, so that it could effectively weaken the occasional correlation problems of the glucose changes and the interference factors which are caused by the absorption of water, albumin, hemoglobin, fat and other components of the blood in human body, the change of temperature of human body, the drift of measuring instruments, the changes of measuring environment, and the changes of measuring conditions; and then a nonlinear quantitative analysis model is built with the near-infrared spectroscopy data after NAP, in order to solve the nonlinear relationship between glucose concentrations and near-infrared spectroscopy which is caused by body strong scattering. In this paper, the new method is compared with other three quantitative analysis models building on partial least squares (PLS), net analyte preprocessing partial least squares (NAP-PLS) and RBFPLS respectively.

Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing.

The objective of this study was to investigate the compositional profiles and microbial shifts of oral microbiota during head-and-neck radiotherapy. Bioinformatic analysis based on 16S rRNA gene pyrosequencing was performed to assess the diversity and variation of oral microbiota of irradiated patients. Eight patients with head and neck cancers were involved in this study.

Exploring the dynamic core microbiome of plaque microbiota during head-and-neck radiotherapy using pyrosequencing.

Radiotherapy is the primary treatment modality used for patients with head-and-neck cancers, but inevitably causes microorganism-related oral complications. This study aims to explore the dynamic core microbiome of oral microbiota in supragingival plaque during the course of head-and-neck radiotherapy. Eight subjects aged 26 to 70 were recruited.

Effects of intensity-modulated radiotherapy on human oral microflora.

This study aimed to evaluate changes in the biodiversity of the oral microflora of patients with head and neck cancer treated with postoperative intensity-modulated radiotherapy (IMRT) or conventional radiotherapy (CRT). Pooled dental plaque samples were collected during the radiation treatment from patients receiving IMRT (n = 13) and CRT (n = 12). Denaturing gradient gel electrophoresis (DGGE) was used to analyze the temporal variation of these plaque samples.

[Influence of Streptococcus mutans luxS gene on polysaccharide matrix metabolism].

Purpose: To investigate the effect of Streptococcus mutans luxS gene on polysaccharide matrix metabolism.

Methods: Based on the immobilization of magnetic beads by adherent cells,an assay of biofilm quantitative analysis was developed for the kinetic quantification of biofilm formation. S.

Modeling of diffusion transport through oral biofilms with the inverse problem method.

Aim: The purpose of this study was to develop a mathematical model to quantitatively describe the passive transport of macromolecules within dental biofilms.

Methodology: Fluorescently labeled dextrans with different molecular mass (3 kD, 10 kD, 40 kD, 70 kD, 2000 kD) were used as a series of diffusion probes. Streptococcus mutans, Streptococcus sanguinis, Actinomyces naeslundii and Fusobacterium nucleatum were used as inocula for biofilm formation.