Monitoring of gastrointestinal carcinoma via molecular residual disease with circulating tumor DNA using a tumor-informed assay.

Background: Circulating tumor DNA (ctDNA)-based minimal residual disease (MRD) detection, which can identify disease relapse ahead of radiological imaging, has shown promising performance. The objective of this study was to develop and validate OriMIRACLE S (Minimal Residual Circulating Nucleic Acid Longitudinal Detection in Solid Tumor), a highly sensitive and specific tumor-informed assay for MRD detection.

Methods: Tumor-specific somatic single nucleotide variants (SNVs) were identified via whole exome sequencing of tumor tissue and matched germline DNA.

Effects of exogenous melatonin on body mass and thermogenesis in red-backed vole (Eothenomys miletus) between Kunming and Dali regions.

Melatonin (MEL) is an indole hormone synthesized and secreted by the pineal gland at night, which is involved in the regulation of body mass and thermogenesis in small mammals. To test the effects of exogenous MEL on body mass and thermogenic ability in two different red-backed vole (Eothenomys miletus) populations from two different regions (Kunming [KM] and Dali [DL]) with different annual variation in climatic variables, such as temperature, sunshine and rainfall. we traced the changes of energy balance in E.

Metabonomics of white adipose tissue and brown adipose tissue in Tupaia belangeri during cold acclimation.

In the present study, liquid chromatography-mass spectrometer (LC-MS) was used to perform untargeted metabolomics analysis of white adipose tissue (WAT) and brown adipose tissue (BAT) in Tupaia belangeri during cold acclimation. Differences in biochemical composition between WAT and BAT were compared. Clarifying how the two adipose tissues respond to the lower temperature in terms of metabolomics, which elucidate the metabolic process and energy homeostasis regulation mechanism in T.

Different Performance of Intravertebral Vacuum Clefts in Kümmell's Disease and Relevant Treatment Strategies.

Objectives: This study aimed to present the different pattern of intravertebral vacuum cleft (IVC) related to high risk of cement complications in minimally invasive treatments for Kümmell's disease (KD) and relevant treatment strategies.

Methods: A retrospective study from January 2016 to January 2018 was conducted at Wuhan Fourth Hospital and comprised 35 patients with Kümmell's disease. There were seven males and 28 females, and the mean age of the patients was 70.

Metabolomics of Eothenomys miletus from five Hengduan Mountains locations in summer.

Climatic characteristics of Hengduan Mountains region were diverse, and Eothenomys miletus was a native species throughout this region. To investigate adaptive strategies of E. miletus to environmental factors in different locations in this region, five locations were selected, including Deqin (DQ), Xianggelila (XGLL), Lijiang (LJ), Jianchuan (JC) and Ailaoshan (ALS).

Role of thermal physiology and bioenergetics on adaptation in tree shrew (Tupaia belangeri): the experiment test.

Ambient conditions, as temperature and photoperiod, play a key role in animals' physiology and behaviors. To test the hypothesis that the maximum thermal physiological and bioenergetics tolerances are induced by extreme environments in Tupaia belangeri. We integrated the acclimatized and acclimated data in several physiological, hormonal, and biochemical markers of thermogenic capacity and bioenergetics in T.

Effects of random food deprivation and refeeding on energy metabolism, behavior and hypothalamic neuropeptide expression in Apodemus chevrieri.

Maintaining adaptive control of behavior and physiology is the main strategy used by animals in responding to changes of food resources. To investigate the effects of random food deprivation (FD) and refeeding on energy metabolism and behavior in Apodemus chevrieri, we acclimated adult males to FD for 4weeks, then refed them ad libitum for 4weeks (FD-Re group). During the period of FD, animals were fed ad libitum for 4 randomly assigned days each week, and deprived of food the other 3days.

Seasonal changes in body mass, serum leptin levels and hypothalamic neuropeptide gene expression in male Eothenomys olitor.

The present study examined seasonal changes in body mass and energy metabolism in the Chaotung vole (Eothenomys olitor) and the physiological mechanisms underpinning these changes. Seasonal changes in the following parameters were measured in male E. olitor, body mass, food intake, thermogenesis, enzyme activity, masses of tissues and organs, hormone concentrations and expression of hypothalamic arcuate nucleus energy balance genes including neuropeptide Y (NPY), agouti-related protein (AgRP), pro-opiomelanocortin (POMC), and cocaine- and amphetamine-regulated transcript (CART).

Benefits of a circular approach to mobilize the thyroid during an endoscopic thyroidectomy.

Background: Mobilization of the thyroid during an endoscopic thyroidectomy (ET) via a breast approach was originally carried out from the lower pole to the upper pole (upward approach). Here, we applied a modified circular approach to achieve better exposure of the surgical field, in which the path of thyroid mobilization started from the isthmus and resembled a circle. The purpose of this study is to evaluate the safety and feasibility of the circular approach compared with the upward approach.

A novel trinuclear cobalt(II) complex derived from an asymmetric Salamo-type N2O3 bisoxime chelate ligand: synthesis, structure and optical properties.

A novel trinuclear Co(II) complex, {[CoL(PrOH)]2Co(H2O)}·PrOH, has been synthesized with 6-ethoxy-6'-hydroxy-2,2'-[ethylenedioxybis(nitrilomethylidyne)]diphenol (H3L), and characterized by elemental analyses, FT-IR, UV-Vis, molar conductance and X-ray crystallographic analysis. Two n-propanol molecules and one water molecule coordinate to three Co(II) ions and four μ-phenoxo oxygen atoms from two [CoL(CH3CH2CH2OH)] units also coordinating to Co(II) ion. All the penta-coordinated Co(II) ions of the Co(II) complex have a slightly distorted trigonal bipyramidal coordinated polyhedron.

Variations in thermal physiology and energetics of the tree shrew (Tupaia belangeri) in response to cold acclimation.

Variations in environmental factors instigate significant changes in the physiology and behavior of animals, necessary for their survival. The present study investigated the hypothesis that ambient temperature is a cue capable of inducing changes in body mass, energy intake, and thermogenic capacity. Moreover, the current study determined the potential role of leptin in regulating adaptive thermogenesis in tree shrews (Tupaia belangeri).