[Expression of multiple tumor suppressor gene p16 and its relationship with prognosis of gastric cancer patients].

Objective: To investigate the relationship between p16 expression and cell proliferation and prognosis in gastric cancer patients.

Methods: Gastric cancer cell lines SGC-7901, MKN45, MKN28, human embryonic kidney cell line HEK293, human fibroblast cell line MRC-5, and surgical specimens of gastric carcinoma and adjacent normal gastric mucosa from 65 patients were included in this study. RT-PCR, MTT and FCM assays were used to detect p16 expression in gastric cancer cell lines and surgical specimens of gastric cancer.