[Construction and identification of recombinant adenoviruses containing the gene fragments encoding Her2/neu extracellular and transmembrane domains].

Objective: To construct eukaryotic expression vectors using recombinant adenovirus containing the gene fragments encoding Her2/neu extracellular first ligand-binding domain (Her2-ECD), full-length extracellular domain (Her2-ECD), and extracellular and transmembrane domain (Her2-TM).

Methods: The cDNAs were amplified by RT-PCR and inserted into shuttle pAdTrack-CMV plasmids. Viral plasmids were obtained from homologous recombination in E.

Human keratinocyte growth inhibition by ultraviolet B and the protective effect of vitamin E.

Objective: To investigate the survival and cell cycle of HaCaT keratinocytes following UVB irradiation at various dosages, and observe the effect of vitamin E(Vit E) on the growth behavior of the cells in this condition.

Methods And Results: MTT assay and flow cytometry were employed to analyze the cell survival ratio and changes in cell growth behavior in various phases of the cell cycle. It was found that for a given length of time of cell incubation following UVB irradiation, the survival ratio of HaCaT keratinocytes decreased gradually as the irradiation dose increased; at a fixed UVB irradiation dose, prolonged incubation of the cells following the irradiation resulted in decreased cell survival ratio, which, however, began to increase when the minimum ratio was reached, with the exception of the cells subjected to irradiation for 15 min.