[Psychological impact on the adolescent patients at the beginning of the fixed orthodontic treatment].

Objective: To assess whether there is a short negative psychological influence on adolescent patients at the beginning of the fixed orthodontic treatment.

Methods: 150 patients (average 14.8 years old) were selected.

[A study on transfection of green fluorescence protein gene into human adipose stromal cells in vitro].

Objective: To make a comparison on the efficiency of two methods for transfecting Green Fluorescence Protein gene into human adipose tissue-derived stromal cells, and to study the biological properties and multipotential differentiation of gene-transfected cells.

Methods: The human subcutaneous adipose tissue was obtained, digested with one volume of collagenase type I, and then cultured with BGJb medium. After subculture and expansion, the human adipose tissue-derived stromal cells infected with Ad-GFP or liposome were observed and analyzed with fluorescence microscopy and flow cytometry to assess transfection efficiency.

[Study on multi-lineage potential of bone marrow mesenchymal stem cells derived from green fluorescent protein transgenic mice].

Objective: To study the multi-lineage potential of bone marrow mesenchymal stem cells (MSCs) derived from transgenic mice with green fluorescent protein (GFP) gene in vitro.

Methods: A 6-week-old GFP transgenic mouse was executed by dislocation of cervical vertebra, and the marrow in tibia and thighbone was washed out with asepsis. The limited cell strains of MSCs derived from GFP transgenic mice (GFP-MSCs) were obtained with density gradient centrifugation.

[A study on transfecting green fluorescent protein gene to rat bone marrow mesenchymal stem cells].

Objective: To study an efficient method to transfect green fluorescent protein gene (GFP) to rat bone marrow mesenchymal stem cells (MSCs) and to determine the biological properties and differentiation potency of transfected MSCs.

Methods: SD rats' bone marrow MSCs were separated and purified in vitro. After subculture and expansion, MSCs infected with Adenoviral vector (Ad-GFP) or transfected with liposome were observed, and their transfection efficiency was assessed with flow cytometry.

[A study on the chondrogenesis of the compound of alginate gelatin and bone marrow stromal cells in vivo].

Objective: To investigate the chondrogenisis by alginate gelatin and rats' bone marrow stromal cells (BMSCs) chondrogenicly induced in vitro.

Methods: Thirty-two male adult SD rats were assigned randomly to experimental and control groups. In experimental group, bone marrow was obtained from the right tibias of all the rats.

[Establishment of a culture system of chick embryo for mouse tooth germ development].

Objective: To establish a new culture system for mouse tooth germs in chick embryo.

Methods: The mandibular first molar germ fragments of 15 embryonic days' Kunming mouse embryo were implanted into the lateral mesenchyme of 4-5 days' chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.