Publications by authors named "Zheng-You Yang"
To investigate the role of Tobacco vein banding mosaic virus (TVBMV) 3'-UTR in virus systemic infection, three types of deletions were introduced into TVBMV infectious clone pCaTVBMV-GFP. Mutants with deletions at the nucleotide position 8-42, 43-141, or 163-174 in the 3'-UTR failed to cause systemic infection in N. benthamiana plants.
View Article and Find Full Text PDFMultiple plant viruses, including potato virus X (PVX), have been modified as vectors for expressing heterologous genes or silencing endogenous genes in plants. PVX-based vectors facilitate the functional analysis of genes in plant. However, they can only express one protein in a time.
View Article and Find Full Text PDF[Comprehensive periodontal treatment of severe chronic periodontitis: a case report with four years follow-up].
Zhonghua Kou Qiang Yi Xue Za Zhi
February 2012
Objective: To investigate the significance of type IV collagen, metalloproteinase-2 (MMP-2), metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) expression in laryngeal squamous cell carcinomas (LSCCs).
Methods: Expression was quantified in 44 LSCC and 22 adjacent non-cancer normal tissues using a streptavidin-peroxidase conjugated immunohistochemistry and associations between the levels of the four proteins and clinicopathological characteristics in LSCC were analyzed.
Results: Significantly different expression of all four proteins was observed in LSCC and adjacent non-cancer normal tissues (P<0.
Rapid detection of enterotoxigenic Clostridium perfringens in meat samples was accomplished with an immunomagnetic separation polymerase chain reaction (IMS-PCR). First, a monoclonal antibody (mAb) specific to C. perfringens was generated.
View Article and Find Full Text PDFArticle Synopsis
- Three detection methods for Listeria monocytogenes in meat samples were developed, including ELISA, ICG strip tests, and IMBS systems, all showing strong reactions with Listeria and weak reactions with Staphylococcus aureus.
- * Combining ELISA and ICG with IMBS reduced the required enrichment time from 24 hours to 14 hours, allowing for faster detection of L. monocytogenes in contaminated meat.
- * The new methods (ELISA-IMBS and ICG-IMBS) demonstrate similar accuracy to standard methods and can detect L. monocytogenes within 15 hours, making them promising for rapid and cost-effective screening in food safety.
Article Synopsis
- Researchers developed a rapid immunochromatography (ICG) strip test to detect aflatoxin B1 (AFB1) using a nanocolloidal gold-antibody probe, achieving a visual detection limit of 0.5 ng/ml.
- The test was designed with a specific monoclonal antibody that reacts not only to AFB1 but also to related aflatoxins B2, G1, and G2.
- Results from the ICG test were consistent with traditional HPLC analysis across 172 grain and feed samples, showing its potential as a fast, cost-effective screening method for mycotoxins in food and agricultural products, with results deliverable in just 15 minutes.
An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L.
View Article and Find Full Text PDFA monoclonal antibody (mab) against the antimicrobial sulfamethazine was prepared and characterized by an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA). Sulfamethazine in the range of 0.2 and 45 ng/ml could be determined with the mab by IC-ELISA.
View Article and Find Full Text PDFAn immunochromatography (ICG) strip test for rapid detection of atrazine in water samples was developed. A monoclonal antibody (MAb) specific to atrazine was produced from the cloned hybridoma cell (AT-1-M3) and used to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and ICG strip. MAb conjugated to colloidal gold, and that was applied to the conjugate pad of the ICG strip.
View Article and Find Full Text PDFTo detect the organophosphorus (OP) pesticide pirimiphos-methyl in grain samples, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed and optimized. By the active esters method, pirimiphos-methyl hapten A was conjugated to keyhole limpet hemocyanin to be used as the immunogen for the production of monoclonal antibodies, and pirimiphos-methyl hapten B was conjugated to ovalbumin to be used as coating antigen. By using the monoclonal antibody and the coating antigen, an IC-ELISA has been developed.
View Article and Find Full Text PDFA direct competitive enzyme-linked immunosorbent assay (ELISA) based on a monoclonal antibody has been developed and optimized for detection of aflatoxin B1 (AFB1), and an ELISA kit has been designed. This immunoassay was highly specific, sensitive, rapid, simple, and suitable for aflatoxin monitoring. AFB1 concentrations determinable by ELISA ranged from 0.
View Article and Find Full Text PDF[Effects of surface potentials of tooth hard tissue on bone remodeling in rabbit tibiae].
Zhonghua Kou Qiang Yi Xue Za Zhi
July 2005
Article Synopsis
- This study explores how the electrical properties of tooth hard tissue influence bone remodeling in rabbits.
- Electrochemical measurements were taken from extracted human teeth, and teeth sections were implanted into the tibiae of 25 rabbits, which were observed over 8 weeks for bone regeneration differences.
- Results showed that the side of the tooth acting as a cathode (cementum) promoted new bone formation, while the anode side (enamel) caused bone resorption, indicating that tooth electrochemistry plays a crucial role in alveolar bone remodeling.
Article Synopsis
- A multiplex PCR assay was developed to identify molds that can produce aflatoxins in Korean fermented foods and grains by targeting three specific genes related to aflatoxin biosynthesis.
- Testing showed that the PCR method effectively detected aflatoxigenic molds like Aspergillus flavus and Aspergillus parasiticus, while other molds tested negative, supported by additional tests (DC-ELISA).
- The study analyzed 22 Meju samples, 24 Doenjang samples, and 10 barley samples, finding aflatoxin in some Meju and barley samples, while Doenjang samples showed low contamination levels overall.