Background: Sepsis-associated encephalopathy (SAE) is among the most prevalent and deadly complications associated with sepsis, but satisfactory treatments and therapeutic agents are lacking. Gelsevirine, an active ingredient derived from Gelsemium elegans Benth., has shown promising effects in animal models of anxiety, ischaemic stroke and osteoarthritis.
View Article and Find Full Text PDFZhongguo Shi Yan Xue Ye Xue Za Zhi
December 2023
Objective: To investigate characteristics of silent alpha thalassemia genes in child-bearing adults in Guangdong, in order to provide data for the prevention and control of hemoglobin H disease.
Methods: A total of 8 752 cases were collected from January 2016 to December 2020. Gap-PCR was used to detect the deletional of α-thalassemia mutations (-α, -α), while PCR reverse dot blot hybridization assay (RDB) was used to detect the non-deletional α-thalassemia mutations (Hb CS, Hb QS and Hb Westmead).
Single-cell analysis of the DNA repair protein is important but remains unachieved. Exploration of nanopipettte technologies in single-cell electroanalysis has recently seen rapid growth, while the θ-nanopipette represents an emerging technological frontier with its potential largely veiled. Here a θ-nanopipette is first applied for single-cell resistive-pulse sensing (RPS) of the important DNA repair protein O-alkylguanine DNA alkyltransferase (hAGT).
View Article and Find Full Text PDFThe use of functional DNA nanostructures as carriers to ship proteins through solid-state nanopores has recently seen substantial growth in single-protein-molecule detection (SPMD), driven by the potential of this methodology and implementations that it may enable. Ultrasmall nanopores have exhibited obvious advantages in spatiotemporal biological detection due to the appropriate nanoconfined spaces and unique properties. Herein, a 6.
View Article and Find Full Text PDFPaclitaxel (PTX) has previously been used to treat tumours of various tissue origins, such as lung, breast, ovarian, prostate cancers and leukemia. PTX-induced apoptosis is associated with p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase (ERK), nuclear factor-kappa B (NF-κB) and c-Jun N-terminal kinase or stress-activated protein kinase (JNK/ SAPK) pathways. Transforming growth factor-beta-activated kinase 1 (TAK1) and TAK1-binding protein 1 (TAB1) play an important role in cell apoptosis through the p38, ERK, NF-κB and JNK signal transduction pathways.
View Article and Find Full Text PDFNan Fang Yi Ke Da Xue Xue Bao
July 2017
Objective: To report the identification of a novel 3.8-kb deletion that caused α thalassemia and establish the method for detecting the deletion fragment.
Methods: Peripheral blood samples were collected from the proband and his mother for analysis of the hematological parameters and routine test for thalassemia genes.
Using the tricyano precursor (Bu(4)N)[(Tp)Cr(CN)(3)] (Bu(4)N(+) = tetrabutylammonium cation; Tp = tris(pyrazolyl)hydroborate), a pentanuclear heterometallic cluster [(Tp)(2)Cr(2)(CN)(6)Cu(3)(Me(3)tacn)(3)][(Tp)Cr(CN)(3)](ClO(4))(3)·5H(2)O (1, Me(3)tacn = N,N',N'-trimethyl-1,4,7-triazacyclononane), three tetranuclear heterometallic clusters [(Tp)(2)Cr(2)(CN)(6)Cu(2)(L(OEt))(2)]·2.5CH(3)CN (2, L(OEt) = [(Cp)Co(P(O)(OEt)(2))(3)], Cp = cyclopentadiene), [(Tp)(2)Cr(2)(CN)(6)Mn(2)(L(OEt))(2)]·4H(2)O (3), and [(Tp)(2)Cr(2)(CN)(6)Mn(2)(phen)(4)](ClO(4))(2) (4, phen = phenanthroline), and a one-dimensional (1D) chain polymer [(Tp)(2)Cr(2)(CN)(6)Mn(bpy)](n) (5, bpy = 2,2'-bipyridine) have been synthesized and structurally characterized. Complex 1 shows a trigonal bipyramidal geometry in which [(Tp)Cr(CN)(3)](-) units occupy the apical positions and are linked through cyanide to [Cu(Me(3)tacn)](2+) units situated in the equatorial plane.
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