Publications by authors named "Zheng-Rong Ding"
Background: This report describes emergency response following an imported vaccine derived poliovirus (VDPV) case from Myanmar to Yunnan Province, China and the cross-border collaboration between China and Myanmar. Immediately after confirmation of the VDPV case, China disseminated related information to Myanmar with the assistance of the World Health Organization.
Methods: A series of epidemiological investigations were conducted, both in China and Myanmar, including retrospective searches of acute flaccid paralysis (AFP) cases, oral poliovirus vaccine (OPV) coverage assessment, and investigation of contacts and healthy children.
To investigate the epidemic and evolutionary trends of enterovirus (EV) in the external environment of Yunnan Province, China, molecular typing was performed on 4 EV strains that were isolated from environmental sewage in Yunnan. The VP1 region of isolates was amplified by RT-PCR using universal enterovirus primers, and the amplified VP1 region was sequenced for GenBank BLAST search and genotype analysis. The 4 EV strains were identified as ECHO7.
View Article and Find Full Text PDFArticle Synopsis
- A study was conducted on 105 non-polio enteroviruses (NPEVs) from Yunnan Province, China, focusing on the VP1 coding region to analyze their genotype distribution and evolution over a five-year period from 2006 to 2010.* -
- Results revealed that the majority (73.3%) of the isolates belonged to human enterovirus B species, followed by A (17.1%) and C (9.5%), with no isolates from enterovirus D species.* -
- Phylogenetic analysis indicated that while different viral serotypes clustered together, Yunnan strains often branched differently from prototype strains, highlighting significant genetic variation even among similar genotypes.*
To explore the enteroviruses surveillance among healthy children under 15 years old in the border areas of Yunnan Province and Myanmar in 2009. The stool samples were collected from the healthy children under 15 years old who came from the border areas of Myanmar and Yunnan Province, virus isolation and sequencing were conducted for all the 271 samples. 6 strains of polioviruses (PVs) were detected from 271 stools with an isolation rate of 2.
View Article and Find Full Text PDF[Distribution of hepatitis B virus genotypes and serotypes in people who had a physical examination in Yunnan Province].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
April 2011
Objective: To know genotypes and serotypes of hepatitis B virus (HBV) detected from hepatitis B infected people in Yunnan Province.
Methods: Serum samples were collected from HBsAg carriers detected from people who had a physical examination at Yunnan Provincial Center for Disease Control and Prevention. The S genes of HBV were amplified by nested PCR and the PCR products were sequenced.
Article Synopsis
- - The study examined the epidemiological features of paralytic cases related to type 2 vaccine-derived poliovirus (VDPV) in Zhaotong city, China, in 2010, focusing on the excretion status of affected individuals and healthy children under five.
- - Researchers collected 108 stool samples (from a case, close contacts, and healthy children) but found no evidence of VDPV or related strains circulating among these groups.
- - They isolated 21 non-polio enteroviruses (NPEVs) from 105 samples, with 52.4% being HEV-A and 47.6% HEV-B, indicating the presence of other enterovirus infections in the area.
Molecular typing was conducted according to the reported method for one HBsAg positive carrier who had a physical examination in Yunnan Province. The S gene of this HBV sample was amplified by nested PCR and the PCR products were directly sequenced. Blast searching was done on the Genbank database and the sequence were compared with the HBV reference sequences in database.
View Article and Find Full Text PDF[Investigation on an outbreak of measles caused by new virus (d11 genotype) imported from Myanmar].
Zhonghua Liu Xing Bing Xue Za Zhi
January 2011
Article Synopsis
- A study investigated a measles outbreak in Menglian County, caused by an imported d11 genotype virus from Myanmar, with a focus on understanding contributing factors and implementing effective control measures.
- The outbreak resulted in 15 reported cases, primarily affecting young children and Myanmar citizens, highlighting gaps in vaccination history, but effective emergency vaccinations helped control the situation.
- The findings stressed the risk of imported measles leading to local epidemics, underscoring the importance of timely vaccination to prevent the spread of the disease and the effectiveness of the measles vaccination in the region.
[Status of enterovirus infection and molecular identification among healthy children at the areas bordering Myanmar, in Yunnan province, China].
Zhonghua Liu Xing Bing Xue Za Zhi
February 2010
Objective: To explore the enterovirus infection status among healthy children under 15 years old in the border areas of Yunnan province that connecting Myanmar.
Methods: A total of 319 stool samples were collected from healthy children in the 10 entrance ports. Enterovirus was isolated from these stool samples and then poliovirus and adenovirus were serotyped by neutralization test using specific anti-sera.
[Characteristics of 57 acute flaccid paralysis cases with polio-virus isolated from stool specimens, in Yunnan province, from 2003 to 2007].
Zhonghua Liu Xing Bing Xue Za Zhi
August 2009
Objective: Study on the epidemiological characteristics of poliomyelitis virus in Yunnan, from 2003 to 2007.
Methods: Surveillance data of acute flaccid paralysis (AFP) cases from year 2003 to 2007 was gathered. All the stool specimens were identified to contain polio virus.
[Study on the influencing factors of hepatitis B vaccine timely birth dose for infants born at hospitals].
Zhongguo Yi Miao He Mian Yi
October 2009
Article Synopsis
- - The study aimed to assess the timely coverage of the HepB vaccine (HepB1) for infants born in hospitals in remote, impoverished areas of Yunnan province and identify reasons for delays in vaccination.
- - Results showed an average HepB1 coverage of 67.19%, with higher rates in county hospitals compared to township hospitals, and key barriers included issues like premature birth and vaccine shortages.
- - To improve HepB1 coverage, the study suggests ensuring a sufficient supply of the vaccine in village hospitals and accurately identifying immunization contraindications as essential strategies.
Molecular typing was conducted according to the reported methods for 2 enteroviruses that were isolated from healthy children in the border areas of Yunnan Province with Myanmar. RT-PCR and sequencing were performed with 292/222 primers according to the Oberste's methods. The resulting sequences were blasted against the Genbank database and compared with all available enterovirus database.
View Article and Find Full Text PDF[Analysis on immune effect of different measles vaccine among 196 infants at age of 8-month in Yunnan Province].
Zhongguo Yi Miao He Mian Yi
August 2009
Objective: To analyze the effect of different dosage of measles vaccines (0.2 ml or 0.5 ml) to measles antibody level in 8 month-old infants and study the relationship between maternal antibody in infants and the immune response to the vaccine.
View Article and Find Full Text PDF[Study on the molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan province, China].
Zhonghua Liu Xing Bing Xue Za Zhi
April 2007
Article Synopsis
- - The report investigates the prevalence and types of enteroviruses in Yunnan province, China, through a study of 210 non-poliovirus strains collected from 1997 to 2004 during paralysis surveillance.
- - Out of the isolates, 198 were molecularly typed, revealing that human enterovirus B species was the most common (75.2%), followed by enterovirus C (12.2%), adenovirus (5.7%), and enterovirus A (2.4%).
- - The study highlights that, despite lacking some epidemiological data on non-polio enteroviruses in Yunnan, molecular typing has significantly improved understanding of the enterovirus landscape in the region.