Publications by authors named "Zheng-Mei Li"
In eukaryotes, the localization of small ribosomal subunits to mRNA transcripts requires the translation of Kozak elements at the starting site. The sequence of Kozak elements affects the translation efficiency of protein synthesis. However, whether the upstream nucleotide of Kozak sequence affects the expression of recombinant proteins in Chinese hamster ovary (CHO) cells remains unclear.
View Article and Find Full Text PDFBackground: The association between bone fracture and cardiovascular diseases is examined in this study. While basic research has established a connection between fractures and heart attacks through the linkage between bones and arteries, population studies have not provided clear evidence. The aim of the present study is to investigate the association between bone fracture and the occurrence of myocardial infarction in a natural population during long-term follow-up.
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- - The study investigates the relationship between high blood pressure (hypertension) and fractures over time, focusing on whether hypertension increases the risk of future fractures and vice versa.
- - Data from the China Health and Nutrition Survey tracked over 10,000 adults from 1997 to 2015, using statistical models to analyze the risks associated with hypertension and fractures.
- - Findings revealed that a history of hypertension is linked to a higher risk of future fractures, but having a fracture does not significantly raise the risk of developing hypertension later on.
Background: Hyperuricemia and dyslipidemia are associated with left ventricular hypertrophy (LVH), while the effect of ApoE gene polymorphism on the correlation between serum uric acid (UA) level and severity of LVH in patients with coronary heart disease (CHD) has not been clarified.
Methods: This was a retrospective observational study of patients with CHD. Patients were divided into groups of ε4 carriers and non-ε4 carriers based on sanger sequencing.
Article Synopsis
- Recombinant therapeutic proteins (RTPs) are crucial for biopharmaceuticals, with Chinese hamster ovary (CHO) cells being the primary host for their production due to their effective growth and human-like modifications.
- Recent advancements have improved the yields and quality of recombinant protein expression in CHO cells, though some proteins remain challenging to express at adequate levels.
- The review discusses the complex process of recombinant protein expression and outlines factors that influence protein production, as well as strategies for enhancing expression levels in CHO cells.
Expression profile of circular RNAs in epicardial adipose tissue in heart failure.
Chin Med J (Engl)
November 2020
Background: Recent studies have reported circular RNA (circRNA) expression profiles in various tissue types; however, circRNA expression profile in human epicardial adipose tissue (EAT) remains undefined. This work aimed to compare circRNA expression patterns in EAT between the heart failure (HF) and non-HF groups.
Methods: RNA-sequencing was carried out to compare circRNA expression patterns in EAT specimens from coronary artery disease cases between the HF and non-HF groups.
Exosomes are attracting considerable interest in the cardiovascular field as the wide range of their functions is recognized in acute myocardial infarction (AMI). However, the regulatory role of exosomal long non-coding RNAs (lncRNAs) in AMI remains largely unclear. Exosomes were isolated from the plasma of AMI patients and controls, and the sequencing profiles and twice qRT-PCR validations of exosomal lncRNAs were performed.
View Article and Find Full Text PDFBackground: This study aims to investigate the associations of different (low/medium/high) levels of fasting triglyceride (TG) levels with cardiovascular endpoints.
Methods: This cohort study comprised of in-service and retired employees of the Kailuan Coal Mine Group, who participated in the health examination conducted in 11 hospitals in the Kailuan region from June 2006 to October 2007 (n=100,271). The study population was divided into five groups according to different TG levels.
The aim of the study was to examine the associations among plasma total homocysteine (tHcy) and blood pressure (BP) stages and brachial-ankle pulse wave velocity (ba-PWV) in a Chinese rural community population. In this cross-sectional study, 2148 rural community subjects with normotension and mild hypertension (HTN) were classified into four groups according to ba-PWV level. Multivariate regression showed that ba-PWV was significantly and independently correlated with tHcy (β = 5.
View Article and Find Full Text PDFDepartments of nursing in Taiwan junior colleges teach a comprehensive range of core competency skills; provide the healthcare system with entry level nursing professionals able to deliver complete care services; and help students prepare for and earn their nursing licenses. Framed by current junior college nursing department curricula and considerations of professional core competency, this paper examines the role of core nursing competencies in college education goals as they influence college curriculum design and clinical practicum programs. The achieved result should be a professional curriculum that incorporates general education, basic medical, and professional nursing elements that is capable of nurturing professional nursing school graduates able to execute their professional duties and earn the respect of patients, their families and society.
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