Interaction between pristine nC and bovine serum albumin by fluorimetry: assessment of inner filter effect corrections.

Introduction: Fluorescence spectrometry is widely used to investigate nanomaterial-protein interactions, a crucial component of nanomaterial safety evaluation. However, the inner filter effect (IFE) significantly distorts fluorescence data during the analysis of fullerene (nC) -protein interactions. Systematic correction methods for this system are rarely reported.

Enhancing the role of MRI in rectal cancer: advances from staging to prognosis prediction.

Rectal cancer (RC) is one of the major health challenges worldwide. Accurate staging, restaging, invasiveness assessment, and treatment efficacy evaluation are crucial for its clinical management. Magnetic resonance imaging (MRI) plays a significant role in these processes.

Dynamic kinetic resolution-mediated synthesis of C-3 hydroxylated arginine derivatives.

Hydroxylated amino acids and their derivatives, including those found in proteins, are important in biology and medicinal chemistry. Incubation of N-acetyl-l-arginine with clavaminic acid synthase, a key oxygenase in clavulanic acid biosynthesis, affords a (3R)-hydroxylated product that is identical to material obtained by total synthesis from Boc-beta alanine. The key step employed dynamic kinetic resolution (DKR) of a β-ketoester precursor, achieved in high diastereomeric and enantiomeric excess using an (R)-SEGPHOS/Ru(II) catalyst.

RNA polymerase II at histone genes predicts outcome in human cancer.

Genome-wide hypertranscription is common in human cancer and predicts poor prognosis. To understand how hypertranscription might drive cancer, we applied our formalin-fixed paraffin-embedded (FFPE)-cleavage under targeted accessible chromatin method for mapping RNA polymerase II (RNAPII) genome-wide in FFPE sections. We demonstrate global RNAPII elevations in mouse gliomas and assorted human tumors in small clinical samples and discover regional elevations corresponding to de novo HER2 amplifications punctuated by likely selective sweeps.

Optimization of Ultrasonic-Enzyme Synergistic Extraction of Proanthocyanidins from Jujube: Purification, Characterization, and Bioactivity Study.

Proanthocyanidins have received extensive attention due to their high functional value, but their sources are limited. Therefore, this experiment studied the preparation, biological activities, and characterization of proanthocyanidins from Chinese jujube ( Mill. cv.

Enhanced circular dichroism multilayer Weyl semimetal material structures optimized with the Runge-Kutta algorithm.

In this paper, a multilayer structure (MS) containing Weyl semimetal materials (WSM) is proposed for separating circularly polarized waves with opposite handedness. The MS configuration is optimized to maximize the circular dichroism (CD) bandwidth using the Runge-Kutta (RUN) algorithm. Both periodic and four quasi-periodic sequences (Fibonacci, Thue-Morse, period-doubling, and Rudin-Shapiro sequences) are considered.

An image dataset for analyzing tea picking behavior in tea plantations.

Tea is an important economic product in China, and tea picking is a key agricultural activity. As the practice of tea picking in China gradually shifts towards intelligent and mechanized methods, artificial intelligence recognition technology has become a crucial tool, showing great potential in recognizing large-scale tea picking operations and various picking behaviors. Constructing a comprehensive database is essential for these advancements.

How far do we still need to go with antibiotics in aquatic environments? Antibiotic occurrence, chemical-free or chemical-limited strategies, key challenges, and future perspectives.

Global consumption and progressive migration of antibiotics through aquatic systems have contributed to their rapid spread, posing significant threats to environmental and human health, and antibiotics have been recognized as emerging pollutants. Hence, extensive approaches have been proposed for antibiotic treatment in water, yielding great achievements. This review systematically summarized current knowledge from contamination characteristics to treatment strategies.

Recent advances in the anti-tumor activities of saponins through cholesterol regulation.

Abnormal cholesterol metabolism has become a popular therapeutic target in cancer therapy. In recent years there has been a surge in interest in the anti-tumor activities of saponins, particularly their ability to disrupt cholesterol homeostasis in tumor cells. Cholesterol regulation by saponins is a complex process that involves multiple mechanisms.

Brain imaging and electrophysiological markers of anaphoric reference during speech production.

Pronouns create cohesive links in discourse by referring to previously mentioned elements. Here, we focus on pronominalization during speech production in three experiments employing ERP and fMRI methodologies. Participants were asked to produce two short sentences describing a man or woman using an object.

NPA7: A Dual Receptor Activating Peptide That Inhibits Cardiac Oxidative Stress.

Background: Cardiomyocyte oxidative stress significantly contributes to the progression of hypertension-induced heart failure, highlighting the need for targeted therapies. We developed a novel peptide, NPA7, that coactivates the GC-A (guanylyl cyclase A)/cGMP and MasR (Mas receptor)/cAMP pathway. This study aimed to test NPA7's ability to inhibit oxidative stress by modulating the p62 (Sequestosome 1)-KEAP1 (Kelch-like ECH-associated protein 1)-NRF2 (nuclear factor erythroid 2-related factor 2) pathway in human cardiomyocytes (HCMs) and a rat model of hypertension.

PPARδ restrains the suppression function of intra-tumoral Tregs by limiting CIITA-MHC II expression.

Regulatory T cells (T cells) play a critical role in suppressing anti-tumor immunity, often resulting in unfavorable clinical outcomes across numerous cancers. However, systemic T depletion, while augmenting anti-tumor responses, also triggers detrimental autoimmune disorders. Thus, dissecting the mechanisms by which T cells navigate and exert their functions within the tumor microenvironment (TME) is pivotal for devising innovative T-centric cancer therapies.

Harmless treatment of cyanide tailings by functional bacteria: Degradation of cyanide and the secondary pollutant ammonia nitrogen.

The eco-friendly treatment of cyanide tailings (CT) using microorganisms is a cost-effective and promising technology. However, this process often generates the secondary pollutants, such as ammonia nitrogen (NH-N), which can adversely impacts the surrounding environment. The accumulation of NH-N is also toxic to cyanide-degrading microorganisms, presenting a significant challenge in achieving simultaneous cyanide degradation and NH₄⁺-N mitigation.

A new endoscopic tumor grading for rectal neuroendocrine tumors: Correlation of vascular pattern with histopathology.

Introduction: The clinical utility of applying endoscopic vascular patterns in rectal neuroendocrine tumor (NET) remains unknown. The aims of this study were to develop a system for utilizing vascular patterns for tumor grade and submucosal invasion depth.

Methods: We retrospectively included patients diagnosed as well-differentiated rectal NET between March 2015 and February 2024.

LRMAHpan: a novel tool for multi-allelic HLA presentation prediction using Resnet-based and LSTM-based neural networks.

Introduction: The identification of peptides eluted from HLA complexes by mass spectrometry (MS) can provide critical data for deep learning models of antigen presentation prediction and promote neoantigen vaccine design. A major challenge remains in determining which HLA allele eluted peptides correspond to.

Methods: To address this, we present a tool for prediction of multiple allele (MA) presentation called LRMAHpan, which integrates LSTM network and ResNet_CA network for antigen processing and presentation prediction.

Diagnostic performance of simultaneous multislice diffusion-weighted imaging in differentiating breast lesions: a systematic review and meta-analysis.

Objectives: To investigate the diagnostic performance of simultaneous multislice diffusion-weighted imaging (SMS-DWI) in differentiating malignant and benign breast lesions, and compare it with conventional single shot and readout segmented echo planar imaging without the SMS technique.

Methods: The literature search was performed in PubMed, Embase, and Web of Science to identify comparative studies reporting the diagnostic performance of SMS-DWI and conventional DWI in patients with breast lesions. Histopathological analysis was used as a reference standard for malignant breast lesions.

Evaluation value of ultrasound on gastrointestinal function in patients with acute heart failure.

Objective: To study the changes in gastrointestinal wall thickness, blood flow, motility, and symptoms in patients with acute heart failure, and to assess gastrointestinal function by ultrasound.

Methods: In this study, patients diagnosed with acute heart failure were selected as the study group, and healthy individuals were selected as the control group. Both groups collected general data and completed the Chinese version of the gastrointestinal symptom rating scale.

Impaired Sequential Working Memory in Patients With Young Onset Parkinson's Disease.

Background: Sequential working memory refers to the cognitive ability to maintain and/or manipulate a set of ordered representations within a short period. It remains unclear whether sequential working memory is impaired in patients with young onset Parkinson's disease (YOPD).

Objectives: The aim of this study is to evaluate the sequential working memory in patients with YOPD.

Signal detection and analysis of sulfasalazine adverse reaction events based on the US FDA adverse event reporting database.

Background: Based on real data from the FDA Adverse Event Reporting System (FAERS), we explored and evaluated the adverse reactions (ADRs) of sulfasalazine (SSZ) in the first quarter of 2004-2023 to provide a reference basis and early warning for the safe use of SSZ in the clinic.

Research Design And Methods: The reporting odds ratio (ROR), the proportional reporting ratio (PRR), the Bayesian confidence propagation neural network (BCPNN), and the empirical Bayesian geometric mean (EBGM) were used as measures of SSZ-associated adverse events (AEs) signals, mining for potential positive signals between SSZ and AE.

Results: In this study, a total of 5327 case reports related to SSZ in the last 20 years involving 25 system organ classes (SOCs) were collected in FAERS.

Subjective time dilation in abstinent patients with alcohol use disorder.

Introduction: Patients with alcohol use disorder (AUD) may have distortions in time perception. This study investigated subjective time dilation (the tendency to perceive a time interval longer than it is) and its association with craving and impulsivity in AUD.

Method: Thirty abstinent male inpatients with AUD (age 29-60 years) and thirty sex-, age-, and education-matched healthy controls completed a temporal generalization task, which assessed the preference (point of subjective equality, PSE) and sensitivity of time perception in the second range.

Neutrophil extracellular traps with low concentrations induce proliferation and migration of human fibroblasts via activating CCDC25/ILK/PI3K/AKT pathway.

Neutrophil plays an important role in the early stage of wound healing. Excessive neutrophil extracellular traps (NETs) which are produced by neutrophil have been proved as a significant unfavorable factor for wound healing by impairing the function of human skin fibroblasts. However, the effect of NETs with low concentrations on fibroblasts remains unclear.

Methamphetamine abuse impairs sequential working memory.

The ability to maintain and manipulate sequential information in working memory, referred to as sequential working memory, plays a vital role in our daily life. While research has shown that methamphetamine abuse affects the neural substrates and the overall functioning of working memory, its specific impact on sequential working memory remains unclear. In this study, we asked 62 abstinent methamphetamine-dependent participants and 59 control participants to complete a digit ordering task in which they saw four digits one-by-one over time and subsequently rearranged them in ascending order.