Chemical constituents of Saxifraga stolonifera (L.) Meeb.

To study the chemical constituents of Saxifraga stolonifera (L.) Meeb., chromatographic techniques were applied to separate and purify the compounds, and their structures were confirmed on the basis of physicochemical properties and spectral data.

Two new dihydrobenzofuran lignans from Rabdosia lophanthoides (Buch.-Ham.ex D.Don) Hara.

Two new dihydrobenzofuran lignanosides, (7R,8S)-4,3',9'-trihydroxyl-3-methoxyl-7,8-dihydrobenzofuran-1'-propylneolignan-9-O-(6-O-syringoyl)-beta-D-glucopyranoside, named lophanthoside B (1) and (7R,8S)-4,9,9'-trihydroxyl-3-methoxyl-7,8-dihydrobenzofuran-1'-propylneolignan-3'-O-beta-D-glucopyranoside (2), an enantiomer of umbroside, along with four known dihydrobenzofuran lignans (3-6), were isolated from 50% acetone extract of Rabdosia lophanthoides (Buch.-Ham.ex D.

Two new secolignans from Selaginella sinensis (Desv.) Spring.

Two new secolignans, 3,4-trans-3-hydroxymethyl-4-[bis(4-hydroxyphenyl)methyl]butyrolactone (1) and 2,3-trans-3,4-trans-2-methoxy-3-hydroxymethyl-4-[bis(4-hydroxyphenyl)methyl]tetrahydrofuran (2), together with six known compounds, were isolated from the whole grass of Selaginella sinensis (Desv.) Spring. Their structures were elucidated by NMR and MS experiments.

Aspirin inhibits proliferation of gemcitabine-resistant human pancreatic cancer cells and augments gemcitabine-induced cytotoxicity.

Aim: To investigate whether aspirin is able to augment gemcitabine-induced cytotoxicity in human pancreatic cancer cells.

Methods: Two gemcitabine-insensitive human pancreatic cancer cell lines, PANC-1 and Capan-1, were used. Cells were treated with either aspirin or gemcitabine alone or both of them.

Anti-angiogenic activity of triptolide in anaplastic thyroid carcinoma is mediated by targeting vascular endothelial and tumor cells.

Triptolide is confirmed to suppress angiogenesis of anaplastic thyroid carcinoma. Here we further expound the precise mechanism involved in this activity. Triptolide downregulated nuclear factor kappa B (NF-kappaB) pathway and its targeting genes associated with endothelial cell mobilization in human umbilical vein endothelial cells (HUVECs) and impaired VEGF expression in thyroid carcinoma TA-K cells.

[Chemical pattern recognition for HPLC fingerprint analysis of Flos Lonicerae Japonicae in different collecting time].

Objective: To compare HPLC fingerprint of Flos Lonicerae Japonicae in different collecting time for determining harvest.

Methods: The data from RP-HPLC were analyzed by similarity evaluation, principle component analysis and system cluster analysis.

Results: The content of chemical composition of Flos Lonicerae Japonicae were vaiance on collecting time.

Flavanone O-glycosides from the rhizomes of Dryopteris sublaeta.

The aim of this study was to look for the chemical constituents from the rhizomes of Dryopteris sublaeta. The fresh plant was extracted twice with boiling water, the extract was concentrated to small volume under reduced pressure at 50 degrees C. The concentrated material was partitioned with ether, ethyl acetate and n-butanol.

Chemical constituents from leaves of Celastrus gemmatus Loes.

To study the chemical constituents from the leaves of Celastrus gemmatus Loes., chromatographic methods were used to isolate and purify the chemical constituents, their structures were elucidated by the physiochemical characteristics and spectral data. Nine compounds were obtained and identified as (-)-massoniresinol 3a-O-beta-D-glucopyranoside (1), ambrosidine (2), isolariciresinol 9-O-beta-D-glucopyranoside (3), kaempferol 3-O-beta-D-glucopyranoside (astragalin) (4), kaempferol 3-O-rutinoside (5), kaempferol 3-O-neohesperidoside (6), apigenin 7-O-beta-D-glucuronide (7), apigenin 7-O-beta-D-glucuronide methyl ester (8) and D-sorbitol (9).

[Experimental study of estrogenic activities of five kinds of Chinese herbal medicines].

Objective: To evaluate the estrogenic activity of several kinds of Chinese herbal medicines.

Method: Use zoopery and reporter gene technique to study the estrogenic activity of five Chinese herbal medicines. Zoopery: weanling female Kunming mice weight 9-12 g were administrated botanical extracts of Selaginella tamariscina, Pinus Massoniana, Corallodiscus flabellate, Dryopteris sublaeta and Leonurus heterophyllus, the positive control group with Nilestriol tablets and control group with water, respectiely.

A new stilbene glycoside from Dryopteris sublaeta.

Aim: To study the chemical constituents of Dryopteris sublaeta Ching et Hsu.

Methods: Fresh plant of Dryopteris sublaeta ching et hsu was extracted twice with boiling water, concentrated to small volume under reduced pressure at 50 degrees C. The concentrated material was partitioned with ether, ethyl acetate, and n-butanol.

A new flavanone from Dryopteris sublaeta.

Aim: To study the chemical constituents of Dryopteris sublaeta Ching et Hsu.

Methods: Fresh plant of Dryopteris sublaeta Ching et Hsu was extracted twice with boiling water, the extract was concentrated to small volume under reduced pressure at 50 degrees C. The concentrated material was partitioned with ether, ethyl acetate, and n-butanol.

[Inclusion constant of beta-CD and paeonol measured by phase solubility method].

Objective: Measure the thermal stability and the forming constant of inclusion complex for paeonol with beta-CD, and investigate interaction between beta-CD and paeonol,

Method: The thermal stability investigated by using different thermal analysis. The forming constant was determined by using phase method.

Result: Comparing with melt temperature of paeonol, that of its inclusion complex increase about 220 degrees C.

[The isolation and identification of a new lignanoside from Selaginella tamariscina (Beauv.) Spring].

Aim: To study the chemical constituents of Selaginella tamariscina (Beauv.) Spring.

Methods: The compounds were isolated and purified by macroporous adsorption resin, Sephadex LH-20 and silica gel column chromatography and identified on the basis of their physicochemical and spectral data.

[One new phenylethanoid glycoside from Corallodiscus flabellata].

Aim: To study the antivirus chemical constituents of Corallodiscus flabellata.

Methods: The compounds were isolated with macroporous absorption resin, Diaion HP-20, Sephadex LH-20, silica gel column chromatography and identified on the basis of their physio-chemical and spectral data.

Results: Four compounds were obtained and identified as 1'-O-beta-D-(3,4-dihydroxyphenyl)-ethyl-6'-O-vanilloylglucopyranoside (1); 1'-O-beta-(4-hydroxyphenyl)-ethyl-beta-D-apiofuranosyl-(1-->2')-glucopyranoside (2); 4-hydroxyphenethyl alcohol (3); bis (2-hydroxyethyl) ether (4).

[Study on the chemical constituents of Selaginella tamariscina (Beauv.) Spring].

Aim: To study the chemical constituents of Selaginella tamariscina (Beauv.) Spring.

Methods: Various chromatographic techniques were used to separate and purify the chemical constituents.

[Isolation and structure identification of the chemical constituents from pine needles of Pinus massoniana Lamb].

Aim: To study the chemical constituents of the water-extracts of the pine needles of Pinus massoniana Lamb..

Methods: Pine needles were collected in Xixia country and extracted with boiling water for two times and the water-extracts were concentrated.

[Isolation and structural identification of C-glycosylflavones from Corallodiscus flabellata].

Aim: To study the chemical constituents of Corallodiscus flabellata.

Methods: Fresh plant of Corallodiscus flabellata was extracted twice with boiling water, concentrated to small volume under reduced pressure at 55 degrees C. The concentrated material was partitioned with ether, ethyl acetate, and n-butanol.

[Isolation and structural identification of chemical constituents from Selaginella tamariscina (Beauv.) Spring].

Aim: To study the chemical constituents of the water-extracts of Selaginella tamariscina (Beauv.) Spring.

Methods: Various chromatographic techniques were used to separate and purify the constituents.

[Isolation and structure identification of lignans from pine needles of pinus massoniana Lamb].

Aim: To study the chemical constituents from the water-extracts of pine needles of Pinus massoniana Lamb.

Methods: Chromatographic techniques were used to separate and purify compounds. Their physico-chemical properties and spectral data (UV, IR, MS, 1H-1H, 13C-1H NMR, DEPT, HMBC etc.

[Two new phenylethanoid glycosides from Corallodiscus flabellata].

Aim: To study the chemical constituents from Corallodiscus flabellata.

Methods: Fresh plant of Corallodiscus flabellata was extracted twice with boiling water, filtered to remove insoluble materials, concentrated under reduced pressure at temperature 55 degrees C to a small volume. The concentrated liquor was subjected to solvent-solvent partitioning using ether, ethyl acetate, and n-butanol (saturated with water).

[Studies on the lignan chemical constituents from pine needles of Pinus massoniana Lamb].

Aim: To study the chemical constituents of the pine needles from Pinus massoniana Lamb..

Methods: Various chromatographic techniques were used for the separation and purification.

[Isolation and structural identification of phenylethanoid glycosides from Corallodiscus flabellata].

Aim: To study the chemical constituents from Corallodiscus flabellata.

Methods: The compounds were isolated and purified by macroporous adsorption resin, silica gel column chromatography and identified on the basis of their physiochemical and spectral data.

Results: Three phenylethanoid glycosides (I-III) were obtained from the n-BuOH fraction of water-extracts.

[Studies on the chemical constituents from herba of Corallodiscus flabellata].

Objective: To study the chemical constituents from Corallodiscus flabellata.

Method: The compounds were isolated with macroporous adsorption resin, silica gel column chromatography and identified on the basis of their physiochemical and spectral data.

Result: Six compounds were obtained and identified as vanillic acid, 3,4-dihydroxyphenylethyl-8-O-beta-D-glucopyranoside, syringic acid, caffeic acid, isoacteoside, ferulic acid.

[Isolation and structure identification of ligan glycosides from pine needles of Pinus massoniana lamb].

Aim: To study the chemical constituents of the pine needles of Pinus massoniana lamb..

Methods: Various chromatographic techniques were used to separate and purify.