Structural insights into Semiliki forest virus receptor binding modes indicate novel mechanism of virus endocytosis.

The Very Low-Density Lipoprotein Receptor (VLDLR) is an entry receptor for the prototypic alphavirus Semliki Forest Virus (SFV). However, the precise mechanisms underlying the entry of SFV into cells mediated by VLDLR remain unclear. In this study, we found that of the eight class A (LA) repeats of the VLDLR, only LA2, LA3, and LA5 specifically bind to the native SFV virion while synergistically promoting SFV cell attachment and entry.

[Salidroside alleviates high glucose and ethanol-induced pyroptosis in insulinoma cells].

This study established a pyroptosis injury model by stimulating insulinoma cells(INS-1) of rats with high glucose(HG) and observed the impact of additional ethanol(ET) exposure on cell pyroptosis, as well as the intervention effect of salidroside(SAL). INS-1 cells were cultured and divided into a normal control group(NG), an HG group, an HG + ET(100 mmol·L~(-1)) group, and an HG + ET + SAL(1-100 μmol·L~(-1)) group. After 72 hours of treatment, cell viability was assessed using the cell counting kit-8(CCK-8) assay.

Effect of 2-Mercapto-1-methylimidazole on the Dual Action of Chemical Mechanical Polishing of Cu and Ta.

When chemical mechanical polishing (CMP) of tantalum (Ta)-based barrier layers is done through silicon via (TSV), it is necessary to control the rate selection of copper (Cu) and Ta to prevent the formation of dishing pit formation due to the rapid removal rate (RR) of copper in the via compared to tantalum outside the via. This paper selected 2-mercapto-1-methylimidazole (TAMZ) as an inhibitor, which has the dual effect of reducing the RR of Cu and increasing the RR of Ta. The experimental results show that the rate selection ratio of Cu and Ta is up to 2.

Kidney outcomes associated with SGLT2 inhibitors compared to other glucose-lowering drugs: a real-world study from China.

Objective: This study aimed to investigate the association between the utilization of Sodium-dependent glucose cotransporters inhibitors (SGLT2i) in real-world settings and kidney outcomes in patients with type 2 diabetes (T2D) and chronic kidney disease (CKD) in mainland China.

Methods: In a retrospective analysis of electronic medical records from West China Hospital of Sichuan University, patients with T2D and CKD were included. Patients were divided into two groups, those initiating treatment with SGLT2i and those receiving other glucose-lowering drugs (oGLDs).

Enhancing access to specialist appointments in tertiary healthcare in Shanghai, China: a structured reservation pathway using digital health technologies.

Objective: The aim of this study is to develop, implement the precise reservation path (PRP) and investigate its prediction function for scheduling shunting patients for specialist appointment registration in Shanghai, China.

Design: The PRP system was built on the hospital's existing information system, integrated with WeChat (WeCom) for user convenience. The outcome analysis employed a mixed-methods approach, integrating quantitative analysis with statistical and machine learning techniques, including multivariate logistic regression, random forest (RF) and artificial neural network (ANN) analysis.

Identification of accident-prone segments using APIU: A case study on highway safety analysis in China.

Objective: This study proposes the accident point interval unit (APIU) method combined with the characteristics of road traffic accidents. The aim is to automatically identify accident aggregation areas, providing basis for highway design and traffic management.

Methods: Historical accident data from a secondary highway in Guizhou Province and an expressway in Guangdong Province over 3 to 4 years were analyzed using APIU to identify accident-prone segments.

[Spatio-temporal Distributions and Source Analysis of Nitrogen in the Surface Water of Dianbu River Basin].

Dianbu River, flowing into the western part of Chaohu Lake, has been heavily polluted, and nitrogen is one of the key factors. During three periods （wet, normal, and dry）, 30 surface water samples were collected from the Dianbu River Basin as the research objects. The water chemistry, multiple stable isotopes （N-NO, O-NO, and N-NH）, and a SIAR mixing model were analyzed not only to understand the spatio-temporal distribution characteristics of nitrogen and its influencing factors but also the sources of nitrogen.

Fish-Finder: A robust small target detection method for aquaculture fish in low-quality underwater images.

Underwater fish object detection serves as a pivotal research direction in marine biology, aquaculture management, and computer vision, yet it poses substantial challenges due to the complexity of underwater environments, occultations, and the small-sized and frequently moving fish in aquaculture. Addressing these challenges, we propose a novel underwater fish object detection algorithm named Fish-Finder. First, we engendered a structure titled "C2fBF," utilizing the dual-path routing attention protocol of BiFormer.

Znf706 regulates germ plasm assembly and primordial germ cell development in zebrafish.

The cell fate of primordial germ cells (PGCs) in zebrafish is pre-determined by maternally deposited germ plasm, which is packaged into ribonucleoprotein complex in oocytes and inherited into PGCs-fated cells in embryos. However, the maternal factors regulating the assembly of germ plasm and PGC development remain poorly understood. In this study, we report that the maternal transcription factor Znf706 regulates the assembly of germ plasm factors into a granule-like structure localized perinuclearly in PGCs during migration.

Transcription regulation of African swine fever virus: dual role of M1249L.

African swine fever virus (ASFV), which poses significant risks to the global economy, encodes a unique host-independent transcription system. This system comprises an eight-subunit RNA polymerase (vRNAP), temporally expressed transcription factors and transcript associated proteins, facilitating cross-species transmission via intermediate host. The protein composition of the virion and the presence of transcription factors in virus genome suggest existence of distinct transcription systems during viral infection.

The nonlineard association between triglyceride to HDL cholesterol ratio and long-term heart disease risk: findings from China Health and Retirement Longitudinal Study (CHARLS).

Objective: The aim of this study was to investigate the relationship between triglyceride to high-density lipoprotein cholesterol ratio (TG/HDL-C) and the risk of cardiovascular disease (CVD) in middle-aged and elderly Chinese population. CVD has high morbidity and mortality in China, with 5.09 million CVD deaths in 2019 and a mortality rate of 364.

Antimicrobial Resistance Trends and Epidemiological Characteristics of Isolates from Intra-Abdominal Infections in China: A 6-Year Retrospective Study (2017-2022).

Antimicrobial resistance represents a continuing threat to the health of patients with intra-abdominal infections (IAIs). This study aimed to provide clinicians with guidance to optimize antibiotic therapy. The clinical data and antibiotic susceptibility results of pathogens from patients with IAIs from 2017 to 2022 were retrospectively collected.

Negative-Free Self-Supervised Gaussian Embedding of Graphs.

Graph Contrastive Learning (GCL) has recently emerged as a promising graph self-supervised learning framework for learning discriminative node representations without labels. The widely adopted objective function of GCL benefits from two key properties: alignment and uniformity, which align representations of positive node pairs while uniformly distributing all representations on the hypersphere. The uniformity property plays a critical role in preventing representation collapse and is achieved by pushing apart augmented views of different nodes (negative pairs).

Association between human herpes simplex virus and severe headache or migraine among aged 20-49 years: a cross-sectional study.

Background And Purpose: Previous studies have shown that human herpes simplex virus (HSV) infection may be associated with the onset of headache or migraine. We aimed to investigate the association between HSV infection and severe headache or migraine.

Materials And Methods: The cross-sectional data on 5,730 participants aged 20-49 years were obtained from the 1999-2004 National Health and Nutrition Examination Survey (NHANES).

Radiomic Machine Learning in Invasive Ductal Breast Cancer: Prediction of Ki-67 Expression Level Based on Radiomics of DCE-MRI.

Purpose: Our study aimed to investigate the potential of radiomics with DCE-MRI for predicting Ki-67 expression in invasive ductal breast cancer.

Method: We conducted a retrospective study including 223 patients diagnosed with invasive ductal breast cancer. Radiomics features were extracted from DCE-MRI using 3D-Slicer software.

Porous Metal Phosphonate Frameworks: Construction and Physical Properties.

ConspectusPorous metal phosphonate frameworks (PMPFs) as a subclass of metal-organic frameworks (MOFs) have promising applications in the fields of gas adsorption and separation, ion exchange and storage, catalysis, sensing, etc. Compared to the typical carboxylate-based MOFs, PMPFs exhibit higher thermal and water stability due to the strong coordination ability of the phosphonate ligands. Despite their robust frameworks, PMPFs account for less than 0.

Understanding the Electrochemical Carbon Dioxide Reduction Reaction Mechanism of Lattice Tuning of Copper by Silver Single-Crystal Surface.

Intermolecular interactions and adsorbate coverage on a metal electrode's surface/interface play an important role in CO reduction reaction (CORR). Herein, the activity and selectivity of CORR on bimetallic electrode, where a full monoatomic Cu layer covers on Ag surface (Cu/Ag) are investigated by using density functional theory calculations. The surface geometric and electronic structure results indicate that there is high electrocatalytic activity for CORR on the Cu/Ag electrode.

Quantitative Changes and Transformation Mechanisms of Saponin Components in Chinese Herbal Medicines during Storage and Processing: A Review.

Saponins are an important class of active components in Chinese herbal medicines (CHMs), which are present in large quantities in , , , etc., and have immune regulation, anti-tumor, anti-inflammatory, anti-cardiovascular disease, and hypoglycemic activities. Storage and processing are essential processes in the production process of CHMs which affect the stability of saponin components and then reduce the medicinal and economic value.

Risk factors and distribution pattern of lateral lymph node recurrence after central neck dissection for cN1a papillary thyroid carcinoma.

Background: The indication and extent of selective lateral neck dissection (LND) for cN1a papillary thyroid carcinoma (PTC) remain uncertain. The present study aimed to identify potential predictors and distribution pattern of lateral lymph node recurrence (LLNR) after central neck dissection in cN1a PTC patients.

Methods: The cN1a PTC patients who underwent initial central neck dissection at our centre were retrospectively reviewed, and the median follow-up period was 6.