Huang-Lian-Jie-Du Decoction ameliorates sepsis through dynamic regulation of immune response and gut microbiota-metabolite axis.

Sepsis remains a life-threatening condition with high mortality rates despite current therapeutic approaches. While Huang-Lian-Jie-Du Decoction (HLJDD), a traditional Chinese medicine formula, has been historically used to treat inflammatory conditions, its therapeutic potential in sepsis and underlying mechanisms remain unexplored. This study investigated HLJDD's comprehensive effects on sepsis pathophysiology using a rat cecal ligation and puncture (CLP) model.

Thymic stromal lymphopoietin modulates T cell response and improves cardiac repair post-myocardial infarction.

Background: The inflammatory response is associated with cardiac repair and ventricular remodeling after myocardial infarction (MI). The key inflammation regulatory factor thymic stromal lymphopoietin (TSLP) plays a critical role in various diseases. However, its role in cardiac repair after MI remains uncertain.

Needlescopic Laparoscope Sleeve Gastrectomy (NLSG): a Potential Surgical Approach.

Background: Laparoscopic sleeve gastrectomy has become the most popular weight loss surgery currently, but it inevitably leaves multiple small scars in the abdomen. Although the appearance of single-incision laparoscopic sleeve gastrectomy achieve better cosmetic results, it may lead to postoperative complications such as umbilical hernia and umbilical infection. The author has developed a new surgical approach that can compensate for these deficiencies, which we called needlescopic laparoscope sleeve gastrectomy (NLSG).

Elucidating the molecular mechanisms of CCR5-containing extracellular vesicles in vitro and in a rat model of experimental Rheumatoid arthritis.

Extracellular vesicles from Rheumatoid arthritis (RA) derived synovial fibroblasts (EVs) have been implicated in the pathogenesis of RA, acting as mediators of cell-to-cell communication. This study aimed to elucidate the role of the chemokine receptor CCR5 and EVs positive for CCR5 (EVs) in RA, focusing on their impact on cartilage destruction and bone erosion in a rat model of Adjuvant-induced arthritis (AIA). In vivo experiments were conducted using AIA rats, treated with either EVs, EVs without CCR5 (EVs), or EVs which encapsulated the CCR5 antagonist Maraviroc.

Elevated Serum Direct Bilirubin and Smoking Status Are Prognostic Factors for EGFR-Mutated Non-Small Cell Lung Cancer Patients Receiving Tyrosine Kinase Inhibitor Therapy.

Lung cancer is one of the leading causes of cancer-related death worldwide [...

Senescent lung fibroblasts in idiopathic pulmonary fibrosis facilitate non-small cell lung cancer progression by secreting exosomal MMP1.

Lung cancer is a fatal complication of idiopathic pulmonary fibrosis (IPF) with a poor prognosis. Current treatments are insufficient in improving the prognosis of lung cancer patients with comorbid idiopathic pulmonary fibrosis (IPF-LC). Senescent fibroblasts, as stromal cells in the tumor microenvironment, influence tumor progression via exosomes.

The Causal Effect Between Human Microbiota and Scabies: A Study from the Genetic Perspective.

Background: Previous studies have indicated that human flora may affect the development of scabies, however, no studies have proven a causal relationship between human flora and scabies, which would be detrimental to future in-depth studies on human flora and scabies.

Methods: Mendelian randomization (MR) was used to analyze the causal effect between human microbiota and scabies, with data on intestinal flora and skin flora from two large published studies and data on scabies from the FinnGen database. Five MR analysis methods were used to increase the reliability of the results, and sensitivity analyses were conducted to increase the robustness of the results.

Assessing the effectiveness and mechanisms of Fe/PS, Fe/HO, and O treatment for water disinfection: Spotlight on VBNC bacteria.

Advanced oxidation processes (AOPs) exhibit significant potential for water disinfection due to their generation of large quantities of highly oxidizing free radicals. However, the neglect of viable but nonculturable (VBNC) cells obscures their true disinfection efficacy and potential environmental health risks. Therefore, the study evaluated the disinfection effectiveness and mechanisms of typical AOPs, including Fe/HO, Fe/persulfate (PS), and O, from the perspective of the production of VBNC bacteria.

Unraveling the Configuration Modulation in Spiro-Based Through-Space Charge Transfer Materials.

Thermally activated delayed fluorescence (TADF) materials hold promise for optoelectronic applications. Among various design strategies, through-space charge transfer (TSCT) systems offer the potential for enhanced performance. However, the relationship between molecular configuration and TSCT properties remains unclear compared to traditional through-band charge transfer materials.

Multistep Growth Pathway of Covalent Organic Framework Onion Nanostructures.

The growth of complex organic macromolecular materials in solution is a pervasive phenomenon in both natural and synthetic systems, yet the underlying growth mechanisms remain largely unresolved. Using liquid-phase transmission electron microscopy (TEM), we elucidate the real-time growth pathways of covalent organic framework (COF) onion nanostructures, which involve graphitic layer formation, subsequent layer attachment, onion ring closure, and structural relaxation. This process is marked by variations in orientation and curvature, driven by the dynamic formation of the COF structure, which further regulates order-disorder transition and defect generation within the framework.

Rationally designed Cas9 enables efficient gene activation and base editing.

Compact and adaptable CRISPR-Cas systems enable genome engineering applications in various contexts via high-efficiency delivery. The adeno-associated virus (AAV) is a widely used delivery system. One of the most compact type II-C Cas9 orthologs-CjCas9, derived from is particularly appealing for AAV delivery.

Metabolic profiling identifies potential biomarkers associated with progression from gestational diabetes mellitus to prediabetes postpartum.

The current study aims to identify potential metabolic biomarkers that predict the progression to prediabetes in women with a history of gestational diabetes mellitus (GDM). We constructed a prediabetes group ( = 42) and a control group ( = 40) based on a2-hour 75 g oral glucose tolerance test for women with a history of GDM from six weeks to six months postpartum, and collected their clinical data and biochemical test results. We performed the plasma metabolomics analysis of the subjects at the fasting and 2-hour post-load time points by using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS).

Enhanced photocurrents for photoelectrochemical immunoassay of alpha-fetoprotein with Pt-functionalized BiOS nanoflowers.

Background: Designing heterojunctions with efficient electron-hole separation holds great promise for improving photoelectric response.

Results: Herein, we reported a multifunctional Pt co-catalyst-modified BiOS nanoflowers (BOS NFs) photocatalytic component for achieving an efficient photoelectric chemistry (PEC) immunosensor for alpha-fetoprotein (AFP). Briefly, the Pt co-catalyst improved the intrinsic band gap structure of BOS on the one hand, and on the other hand, it was able to achieve a rapid decomposition of hydrogen peroxide to hydroxyl radicals, which led to the improvement of electrochemical half-responses during the amplification of target immunosignals.

Isovalerylspiramycin I suppresses small cell lung cancer proliferation via ATR/CHK1 mediated DNA damage response and PERK/eIF2α/ATF4/CHOP mediated ER stress.

Small cell lung cancer (SCLC) urgently needs new therapeutic approaches. We found that the antibiotic-derived compound Isovalerylspiramycin I (ISP-I) has potent anti-tumor activity against SCLC cell lines H1048 and DMS53 both in vitro and in vivo. ISP-I induced apoptosis, G2/M phase cell cycle arrest, and mitochondrial respiratory chain dysfunction in both cell lines.

Enhancing the magnetic properties of Dy(III) single-molecule magnets in octahedral coordination symmetry by tuning the equatorial ligands.

Conventionally, octahedral () coordination symmetry of lanthanide centers is not ideal for constructing high-performance single-molecule magnets (SMMs). However, introducing a strong ligand field in the axial direction to increase crystal field splitting can potentially overcome this limitation. Herein, we successfully obtained two dysprosium(III) single-molecule magnets, [Dy(OCBu)X(py)] (X = Cl (1), I (2), py = pyridine), in coordination symmetry.

A Novel Non-Invasive Murine Model of Neonatal Hypoxic-Ischemic Encephalopathy Demonstrates Developmental Delay and Motor Deficits with Activation of Inflammatory Pathways in Monocytes.

Neonatal hypoxic-ischemic encephalopathy (HIE) occurs in 1.5 per 1000 live births, leaving affected children with long-term motor and cognitive deficits. Few animal models of HIE incorporate maternal immune activation (MIA) despite the significant risk MIA poses to HIE incidence and diagnosis.

The potential of flavonoids to mitigate cellular senescence in cardiovascular disease.

Aging is one of the most significant factors affecting cardiovascular health, with cellular senescence being a central hallmark. Senescent cells (SCs) secrete a specific set of signaling molecules known as the senescence-associated secretory phenotype (SASP). The SASP has a remarkable impact on age-associated diseases, particularly cardiovascular diseases (CVD).