Functional verification of a landmark gene EVM713 involved in spermatogenesis in the marine bivalve Chlamys nobilis.

The formation of broodstock gametes is closely linked to the yield and quality in aquaculture production, yet molecular mechanisms underlying this process remain insufficiently understood. The noble scallop Chlamys nobilis, an economically significant dioecious bivalve species, serves as an excellent model for studying gametogenesis. In this study, the adult scallops with testis at different developmental stage were chosen for histological examination and transcriptome analysis to dig genes related gonad development.

Srebp-1 bridges gonad development and lipid accumulation by regulating lipogenesis in noble scallop Chlamys nobilis.

In bivalve, development of female gonad is accompanied with accumulating lipids which provided energy resource for non-feeding larvae development. As the major transcriptional regulators of lipid metabolism, Srebps play pivotal role in lipid homeostasis during oogenesis. However, little work was conducted on Srebps function in bivalves.

Effects of chronic cold stress on tissue structure, antioxidant response, and key gene expression in the warm-water bivalve Chlamys nobilis.

As ectothermic invertebrates, mollusks are regarded as good environmental indicator species for determining the adverse effects of climate change on marine organisms. In the present study, the effects of cold stress on the tissue structure, antioxidant activity, and expression levels of genes were evaluated in the warm-water noble scallop Chlamys nobilis by simulating natural seawater cooled down during winter from 17 °C to 14 °C, 12 °C, 10 °C, and 9 °C. Firstly, the gill was severely damaged at 10 °C and 9 °C, indicating that it could be used as a visually indicative organ for monitoring cold stress.

Genome-wide identification of STATs and analysis of their role in sex determination in Pacific oysters (Crassostrea gigas).

STAT (signal transducer and activator of the transcription) proteins, are a group of highly conserved transcription factors and fundamental components of the JAK-STAT signaling pathway. They play crucial roles in a variety of biological processes, such as immunity, proliferation, differentiation, and growth. However, little information is known regarding their role in gonad development and sex determination in mollusks.

Genome-wide identification and expression analysis of Dmrt gene family and their role in gonad development of Pacific oyster (Crassostrea gigas).

Doublesex and Mab-3-related transcription factor (Dmrt) is a type of transcription factor with a zinc-finger DM structural domain, which plays a significant role in sex determination and differentiation in animals. Although Dmrt has been reported in many vertebrates and invertebrates, it has rarely been studied in bivalves. In this study, a total of three members of the Dmrt gene family were identified and characterized in Crassostrea gigas, and all these CgDmrt genes contained a conserved DM domain.

Transcriptome analysis provides insights into the high ability to synthesize fatty acids in "yellow oil" mud crab (Scylla paramamosain).

Yellow oil mud crab (YOC) is a new variant of mud crab (Scylla paramamosain), which was attracted much attention in recent years due to its high level of nutrition. However, the nutritive values and the physiological changes in YOC have not been clearly understood. In this study, we aimed to identify the nutrient compositions (including total carotenoid content (TCC), total lipid content (TLC), total antioxidant capacity (TAC), and fatty acids) and differences in genes related to the biosynthesis of fatty acids using transcriptome analysis in YOC in comparison with those of normal mud crabs.

Effects of high stocking density on growth performance and expression of MyD88, and its temporal expression under the challenge of Vibrio parahaemolyticus in the noble scallop Chlamys nobilis.

High stocking density has been regarded as an adverse factor in bivalve aquaculture. However, its subsequent molecular response to pathogenic bacteria has been little studied. In order to study the question, a novel MyD88 was first cloned using adult noble scallops Chlamys nobilis (CnMyD88), and its tissue distribution was investigated.

In vitro fermentation of Gracilaria lemaneiformis and its sulfated polysaccharides by rabbitfish gut microbes.

This study intended to characterize the Gracilaria lemaneiformis (SW)-derived polysaccharide (GLP) and explore the fermentation aspects of SW and GLP by rabbitfish (Siganus canaliculatus) intestinal microbes. The GLP was mainly composed of galactose and anhydrogalactose (at 2.0:0.

Evaluation of the Feasibility of Harvest Optimisation of Soft-Shell Mud Crab () from the Perspective of Nutritional Values.

Soft-shell crabs have attracted consumers' attention due to their unique taste and nutritional value. To evaluate the feasibility of harvest optimisation of soft-shell mud crabs, the proximate composition, mineral composition, and total carotenoid, amino acid, and fatty acid contents of edible parts of male and female soft-shell mud crabs at different moulting stages were determined and compared from a nutritional value perspective. The results showed that the sex and moulting stages could significantly affect the nutritional values of the edible portions of soft-shell crabs.

Carotenoid content and composition: A special focus on commercially important fish and shellfish.

Carotenoids are natural pigments that provide many health benefits to living organisms. Although terrestrial plants are the major dietary source of carotenoids for humans, aquatic animals (especially fish and shellfish) are equally important because they are rich in certain important carotenoids lacking in fruits and vegetables. Although extensive research has focused on exploring the carotenoid content and composition in fish and shellfish, this information is poorly organized.

Whole-transcriptome RNA sequencing revealed the roles of chitin-related genes in the eyestalk abnormality of a novel mud crab hybrid (Scylla serrata ♀ × S. paramamosain ♂).

Chitin is a kind of insoluble structural polysaccharide and plays different roles in different species. In crustaceans, it forms the structural components in the exoskeleton. In our previous studies, novel mud crab hybrids have been produced from the interspecific hybridization of Scylla serrata ♀ × S.

Intraspecific hybridization as a mitigation strategy of ocean acidification in marine bivalve noble scallop Chlamys nobilis.

The driving factors of climate change, especially ocean acidification (OA), have many detrimental impacts on marine bivalves. Hybridization is one of the important methods to improve environmental tolerance of animals and plants. In this study, we explored the feasibility of intraspecific hybridization as an OA mitigation strategy in noble scallop Chlamys nobilis (ecologically and economically important bivalve species).

Climate change and n-3 LC-PUFA availability.

Omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) are essential fatty acids for the growth, development and survival of virtually all organisms. There is increasing evidence that anthropogenic climate change has a direct and indirect impact on the availability of natural n-3 LC-PUFA. However, this information is fragmented and not well organized.

Evidence of Sex Differentiation Based on Morphological Traits During the Early Development Stage of Mud Crab .

In order to uncover the sexual difference in morphology and how early they appear during the development stage of mud crab , we measured, observed, and biostatistically analyzed morphological traits related to sex. For unveiling the morphological differences between sexes, morphological traits involving abdomen width (AW), carapace length (CL), and carapace width (CW) were first measured during the crablet development stage of in the present study. The correlation analyses and path analyses exhibited that sexual dimorphism in the third abdomen width (AW3) and fourth abdomen width (AW4) could be used for sex identification from stage C VI (stage VI of crablet).

Lipid nutritional quality of marine and freshwater bivalves and their aquaculture potential.

Omega-3 Long-chain polyunsaturated fatty acids (n-3 LC-PUFA) are beneficial to human health. Since the industrial revolution, with the tremendous increase of human population, the supply of natural n-3 LC-PUFA is far lower than the nutritional need of n-3 LC-PUFA. Therefore, a new alternative source of natural n-3 LC-PUFA is urgently needed to reduce the supply and demand gap of n-3 LC-PUFA.

Endogenous LC-PUFA biosynthesis capability in commercially important mollusks.

Mollusks are excellent dietary sources for LC-PUFA. However, the main challenge limiting mollusk production is the high mortality rate of molluskan larvae in early life cycle stages. This paper reviews scientific evidences on molecular and biochemical studies of LC-PUFA biosynthesis in commercially important molluskan species.

The functional roles of the non-coding RNAs in molluscs.

This review focus on the current knowledge of non-coding RNAs (ncRNAs) in molluscs. In this review, we provide an overview of long ncRNAs (lncRNA), microRNAs (miRNA) and piwi-interacting RNAs (piRNA), followed by evidence for the regulation of ncRNAs in variety of biological process in molluscs, including development, biomineralization and innate immune response. This review advances our understanding on the roles of ncRNAs in molluscs and suggest the future direction to fully understand the epigenetic regulatory network of molluscs.

Potential causative factors of noble scallop Chlamys nobilis mass mortality in Nan'ao Island, Shantou, China in 2017.

Noble scallop Chlamys nobilis is an important marine bivalve that has been extensively cultured in the south coast of China since the 1980s. Unfortunately, since the late 1990s, the farmed scallops often suffered from regional mass mortality, which results in enormous economic losses to farmers and industries. In 2017, another mass mortality event occurred in Nan'ao Island, Shantou, China.

mRNA profile provides novel insights into stress adaptation in mud crab megalopa, Scylla paramamosain after salinity stress.

Background: Mud crab, Scylla paramamosain, a euryhaline crustacean species, mainly inhabits the Indo-Western Pacific region. Wild mud crab spawn in high-salt condition and the salinity reduced with the growth of the hatching larvae. When the larvae grow up to megalopa, they migrate back to estuaries and coasts in virtue of the flood tide, settle and recruit adult habitats and metamorphose into the crablet stage.

Differential responses of a pi-class glutathione S-transferase (CnGSTp) expression and antioxidant status between golden and brown noble scallops under pathogenic stress.

Glutathione S-transferases (GSTs) play important roles in immunity by protecting organisms against the damage of reactive oxygen species (ROS). In this study, a pi-class GST cDNA sequence was first cloned from noble scallop Chlamys nobilis (named CnGSTp). The full length cDNA of CnGSTp was 922 bp, encoding a cytosolic protein of 202 amino acids residues, with predicted molecular masses of 23.

Effects of high stocking density on the galectin gene expression in noble scallop Chalmys nobilis under bacterial infection.

High stocking densities have been shown to have adverse effects on the physiology of bivalves. The noble scallop Chlamys nobilis is one of the most important cultured shellfish in Southern China. However, the effects of scallop stocking density on its immunity is not well understood.