Rational design of Ag-doped MnFeO/HNTs for peroxidase-mimicking activity and colorimetric sensing of uric acid in human serum.

Mimicking enzyme have significantly advanced sensing assays by replicating native enzyme functions, yet achieving both high catalytic activity and easy recyclability remains a challenge. In this study, Ag-doped MnFeO/halloysite nanotubes (HNTs) were rationally designed as a novel nanozyme by depositing in-situ Ag and MnFeO nanoparticles onto HNTs. The resulting nanocomposite exhibited excellent peroxidase-like activity along with magnetic properties.

Development of an inducible Cas9 nickase and PAM-free Cas12a platform for bacterial diagnostics.

Rapid, efficient, specific and sensitive diagnostic techniques are critical for selecting appropriate treatments for drug-resistant bacterial infections. To address this challenge, we have developed a novel diagnostic method, called the Dual-Cas Tandem Diagnostic Platform (DTDP), which combines the use of Cas9 nickase (Cas9n) and Cas12a. DTDP works by utilizing the Cas9n-sgRNA complex to create a nick in the target strand's double-stranded DNA (dsDNA).

Dissipation rate and residual fate of thiamethoxam in tobacco leaves and soil exposed to field treatments.

A two-year field experiment was conducted in two different locations to investigate the dissipation rate and residual fate of thiamethoxam in tobacco leaves and soil by high performance liquid chromatography with UV detection. The average recoveries for green, cured tobacco leaves and soil ranged from 89.7 %-94.