Genetic profiling of Gleason grade 4/5 prostate cancer: which is the best prostatic control tissue?

Purpose: We examined the variation in gene expression profiles of prostate cancer caused by zone specific genes.

Materials And Methods: Ten normal central zone, 10 transition zone (benign prostatic hyperplasia) and 6 normal peripheral zone tissues from radical retropubic prostatectomies were compared to each other and to 12 peripheral zone Gleason grade 4/5 cancers. Test chips and HuGeneFL6800 (Affymetrix, Inc.

Hepsin and maspin are inversely expressed in laser capture microdissectioned prostate cancer.

Purpose: Recent studies have shown that hepsin, a serine protease, is over expressed in prostate cancers, implicating hepsin activity in tumor invasion. Using microarray technology we have previously identified 22 genes that were up-regulated in high grade prostate cancers compared with benign prostatic hyperplasia. Of them hepsin was the most differentially over expressed.