Research Note: Synergistic effect of isopropoxy benzene guanidine and colistin against mcr-1-positive Escherichia coli in vitro and in duck intestine infection models.

Colistin (CST) is considered as "agent of last resort" against gram-negative bacteria as feed additive. Its clinical effectiveness has reduced since the emergence of mcr-1 gene in ducks. Isopropoxy benzene guanidine (IBG), a new guanidine derivative, showed positive effects on improving animal weights and alleviating intestinal pathogens, therefore, the objective of this study was to evaluate the effect of this compound supplement with CST in ducks and explore the possibilities in feed additive.

Rapid Dissemination of Gene among Carbapenem-Resistant Isolates in a Yellow-Feather Broiler Farm via Multiple Plasmid Replicon.

Although carbapenems have not been approved for animal use, carbapenem-resistant (CREC) strains are increasingly being detected in food-producing animals, posing a significant public health risk. However, the epidemiological characteristics of CREC isolates in yellow-feather broiler farms remain unclear. We comprehensively investigated the genetic features of carbapenem-resistance genes among isolates recovered from a yellow-feather broiler farm in Guangdong province, China.

Molecular epidemiology and transmission of rmtB-positive Escherichia coli among ducks and environment.

This study aimed to investigate the transmission and molecular epidemiological characteristics of the rmtB gene in Escherichia coli (E. coli) strains isolated from duck farms in Guangdong Province of China from 2018 to 2021. A total of 164 (19.

Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine Isolates in Guangdong, China.

Carbapenem resistance has posed potential harmful risks to human and animals. The objectives of this study were to understand the prevalence of in pigs and investigate the molecular characteristics of NDM-5-producing isolates in Guangdong province in China. Carbapenem-resistant isolates were isolated from pigs and obtained using MacConkey plates containing 0.

Identification of the Multiresistance Gene in Oxazolidinone-Susceptible and of Pig and Feed Origins.

Previous studies on the prevalence and transmission mechanism of oxazolidinone resistance gene in CoNS are lacking, which this study addresses. By screening 763 CoNS isolates from different sources of several livestock farms in Guangdong, China, 2018-2020, we identified that the was present in seven CoNS isolates of pig and feed origins. Species identification and multilocus sequence typing (MLST) confirmed that seven -positive CoNS isolates were composed of five ST64- and two isolates.

Rapid Increase in the IS-Mediated Gene in Isolates with IncP and IncX4 Plasmids and Co-Existing and Genes in a Swine Farm.

This paper aimed to investigate the molecular epidemiological features of the gene in isolates in a typical swine farm during 2014-2017. A total of 617 isolates were screened for the gene using PCR amplification. A susceptibility test, pulsed-field gel electrophoresis (PFGE), S1-PFGE, southern blotting hybridization, and the genetic context of the gene were all used for analyzing all -positive isolates.

Preliminary view of the global distribution and spread of the tet(X) family of tigecycline resistance genes.

Background: The emergence of plasmid-mediated tet(X3)/tet(X4) genes is threatening the role of tigecycline as a last-resort antibiotic to treat clinical infections caused by XDR bacteria. Considering the possible public health threat posed by tet(X) and its variants [which we collectively call 'tet(X) genes' in this study], global monitoring and surveillance are urgently required.

Objectives: Here we conducted a worldwide survey of the global distribution and spread of tet(X) genes.

Reversal of -Mediated Colistin Resistance in by CRISPR-Cas9 System.

Purpose: The plasmid-borne mobilized colistin resistance gene () was discovered in 2015. Subsequently, the rapid horizontal transfer of gene to diverse bacterial species poses a serious threat to public health, which urgently needs the introduction of novel antimicrobial strategies. Therefore, the purpose of this study is to sensitize bacteria to colistin and reduce the propagation of gene by curing -harboring plasmid in () using the CRISPR-Cas9 system.

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in from Various Veterinary Hospitals.

() is one of the most clinically important zoonotic pathogens, but an understanding of the prevalence, biofilm formulation ability, virulence, and antimicrobial resistance genes of from veterinary hospitals is lacking. By characterizing in different origins of veterinary hospitals in Guangzhou, China, in 2019, we identified with the presence of in pets (17.1%), veterinarians (31.

Clonal Spread of ST93 Carrying -Harboring IncN1-IncHI2/ST3 Plasmid Among Companion Animals, China.

The purpose of this study was to investigate the occurrence of plasmid-mediated colistin resistance gene in Enterobacteriaceae isolates from companion animals in Guangzhou, China. Enterobacteriaceae isolated from 180 samples collected from cats and dogs were screened for by PCR and sequencing. MCR-1-producing isolates were further characterized by multilocus sequence typing and pulsed-field gel electrophoresis (PFGE).

Evolution and Comparative Genomics of F33:A-:B- Plasmids Carrying or in and Isolated from Animals, Food Products, and Humans in China.

To understand the underlying evolution process of F33:A-:B- plasmids among isolates of various origins in China, the complete sequences of 17 -harboring F33:A-:B- plasmids obtained from and isolates from different sources (animals, animal-derived food, and human clinics) in China were determined. F33:A-:B- plasmids shared similar plasmid backbones comprising replication, leading, and conjugative transfer regions and differed by the numbers of repeats in and and by the presence of group II intron, except that pHNAH9 lacked a large segment of the leading and transfer regions. The variable regions of F33:A-B- plasmids were distinct and were inserted downstream of the addiction system /, identified as the integration hot spot among F33:A-B- plasmids.

The role of wildlife (wild birds) in the global transmission of antimicrobial resistance genes.

Antimicrobial resistance is an urgent global health challenge in human and veterinary medicine. Wild animals are not directly exposed to clinically relevant antibiotics; however, antibacterial resistance in wild animals has been increasingly reported worldwide in parallel to the situation in human and veterinary medicine. This underlies the complexity of bacterial resistance in wild animals and the possible interspecies transmission between humans, domestic animals, the environment, and wildlife.