Bioinformatics analysis of the role of lysosome-related genes in breast cancer.

This study aimed to investigate the roles of lysosome-related genes in BC prognosis and immunity. Transcriptome data from TCGA and MSigDB, along with lysosome-related gene sets, underwent NMF cluster analysis, resulting in two subtypes. Using lasso regression and univariate/multivariate Cox regression analysis, an 11-gene signature was successfully identified and verified.

Tips and Tricks: Evolution of Orchidectomy.

Radical orchidectomy has been the standard surgery for testicular tumours. While a straightforward routine surgery, there are several finer points in the surgical technique and perioperative care that urologists should be familiar with. This mini-review discusses modifications to the conventional surgical approach such as organ-sparing surgery and the subinguinal approach, and practice points regarding prostheses and sperm banking that are pertinent to early management of a patient with a testicular tumour.

Dynamic interactions between E-cadherin and Ankyrin-G mediate epithelial cell polarity maintenance.

E-cadherin is an essential cell‒cell adhesion protein that mediates canonical cadherin-catenin complex formation in epithelial lateral membranes. Ankyrin-G (AnkG), a scaffold protein linking membrane proteins to the spectrin-based cytoskeleton, coordinates with E-cadherin to maintain epithelial cell polarity. However, the molecular mechanisms governing this complex formation and its relationships with the cadherin-catenin complex remain elusive.

Modulus difference-induced embedding strategy to construct iontronic pressure sensor with high sensitivity and wide linear response range.

Sensitivity and linearity are two crucial indices to assess the sensing capability of pressure sensors; unfortunately, the two mutually exclusive parameters usually result in limited applications. Although a series of microengineering strategies including micropatterned, multilayered, and porous approach have been provided in detail, the conflict between the two parameters still continues. Here, we present an efficient strategy to resolve this contradiction via modulus difference-induced embedding deformation.

Fast and sensitive coulometric signal transduction for ion-selective electrodes by utilizing a two-compartment cell.

Here, we propose a fast and sensitive coulometric signal transduction method for ion-selective electrodes (ISEs) by utilizing a two-compartment cell. A potassium ion-selective electrode (K-ISE) was connected as reference electrode (RE) and placed in the sample compartment. A glassy carbon (GC) electrode coated with poly(3,4-ethylenedioxythiophene) (GC/PEDOT), or reduced graphene oxide (GC/RGO), was connected as working electrode (WE) and placed in the detection compartment together with a counter electrode (CE).

Tumors evade immune cytotoxicity by altering the surface topology of NK cells.

The highly variable response rates to immunotherapies underscore our limited knowledge about how tumors can manipulate immune cells. Here the membrane topology of natural killer (NK) cells from patients with liver cancer showed that intratumoral NK cells have fewer membrane protrusions compared with liver NK cells outside tumors and with peripheral NK cells. Dysregulation of these protrusions prevented intratumoral NK cells from recognizing tumor cells, from forming lytic immunological synapses and from killing tumor cells.

Recyclable Composite Membrane of Polydopamine and Graphene Oxide-Modified Polyacrylonitrile for Organic Dye Molecule and Heavy Metal Ion Removal.

Developing efficient and recyclable membranes for water contaminant removal still remains a challenge in terms of practical applications. Herein, a recyclable membrane constituted of polyacrylonitrile-graphene and oxide-polydopamine was fabricated and demonstrated efficient adsorption capacities with respect to heavy metal ions (62.9 mg g of Cu ion, CuSO 50 mg L) and organic dye molecules (306.

[Effect of acupuncture on expression of endoplasmic reticulum Ca and Caspase-12 protein in hippocampal neurons of rats with convulsive brain injury].

Objective: To observe the effect of acupuncture on endoplasmic reticulum calcium, apoptosis number and Caspase-12 protein expression in hippocampal neurons of convulsive rats, so as to explore its mechanisms underlying improvement of convulsion.

Methods: SD rats were randomly divided into normal control, model and acupuncture groups, with 36 rats in each group. Rats in the normal control group received intraperitoneal injection (i.

A wearable electrochemical sensor based on β-CD functionalized graphene for pH and potassium ion analysis in sweat.

Here, we developed a wearable electrochemical sensor for pH and K monitoring in sweat. The sensor was composed of flexible reference electrode, pH response electrode and K selective electrode, which were prepared through printing β-CD functionalized graphene (β-CD/RGO) water suspension on conductive PET substrate with microelectronic printer. β-CD/RGO not only served as the pH sensitive material for pH response electrode with good sensitivity, selectivity and reproducibility due to its abundant oxygen-containing functional groups, but also worked as the ion-to-electron transducer for K selective electrode with good sensitivity.

Achieving Record Efficiency and Luminance for TADF Light-Emitting Electrochemical Cells by Dopant Engineering.

Thermally activated delayed fluorescence (TADF) light-emitting electrochemical cells (TADF-LECs) are appealing due to their simple sandwich structure and potential applications in wearable displays and sensors. However, achieving high performance remains challenging. In this paper, we demonstrate that the use of TADF emitters with a low aggregated-caused quenching (ACQ) tendency is crucial to address this challenge.

Role of Two G-Protein α Subunits in Vegetative Growth, Cell Wall Integrity, and Virulence of the Entomopathogenic Fungus .

Heterotrimeric G-proteins are crucial for fungal growth and differentiation. The α subunits of heterotrimeric G-proteins play an essential role in controlling signal transduction. However, the function of G-protein α subunits in entomopathogenic fungi remains poorly understood.

Bismuth Nanoparticles Encapsulated in Nitrogen-Rich Porous Carbon Nanofibers as a High-Performance Anode for Aqueous Alkaline Rechargeable Batteries.

The aqueous alkaline rechargeable batteries (AARBs) have an attractive potential for electrochemical energy storage devices. In view of the advantages of high theoretical capacity and desirable negative operating window, bismuth (Bi) has been deemed as a hopeful anode material for AARBs. Unfortunately, intensive reported works of Bi anode are still confronted with limited capacity and poor cycling stability.

The Cdc42 GTPase-activating protein Rga6 promotes the cortical localization of septin.

Septins are a family of filament-forming GTP-binding proteins that regulate fundamental cellular activities, such as cytokinesis and cell polarity. In general, septin filaments function as barriers and scaffolds on the cell cortex. However, little is known about the mechanism that governs the recruitment and localization of the septin complex to the cell cortex.

Postmortem high-dimensional immune profiling of severe COVID-19 patients reveals distinct patterns of immunosuppression and immunoactivation.

A complete diagnostic autopsy is the gold-standard to gain insight into Coronavirus disease 2019 (COVID-19) pathogenesis. To delineate the in situ immune responses to SARS-CoV-2 viral infection, here we perform comprehensive high-dimensional transcriptional and spatial immune profiling in 22 COVID-19 decedents from Wuhan, China. We find TIM-3-mediated and PD-1-mediated immunosuppression as a hallmark of severe COVID-19, particularly in men, with PD-1 cells being proximal rather than distal to TIM-3 cells.

[Effect of acupuncture serum on expression of microtubule associated protein-2 and nerve growth associated protein-43 in Mg-free-cultured hippocampal neurons of neonatal rats].

Objective: To observe the effect of acupuncture serum on the expression of microtubule associated protein-2 (MAP-2) and nerve growth associated protein-43 (GAP-43) in cultured hippocampal neurons of convulsive rats.

Methods: The acute convulsion model was induced by intraperitoneal injection of pentylenetetrazol in SD rats who were then randomized into model group and acupuncture group. Rats of the acupuncture group received manual acupuncture stimulation of "Baihui" (GV20) and "Dazhui" (GV14) for 30 min, once daily for 7 days.

Self-Healing of a Covalently Cross-Linked Polymer Electrolyte Membrane by Diels-Alder Cycloaddition and Electrolyte Embedding for Lithium Ion Batteries.

Thermally reversible self-healing polymer (SHP) electrolyte membranes are obtained by Diels-Alder cycloaddition and electrolyte embedding. The SHP electrolytes membranes are found to display high ionic conductivity, suitable flexibility, remarkable mechanical properties and self-healing ability. The decomposition potential of the SHP electrolyte membrane is about 4.

Graphite-like Carbon Nitride Nanotube for Electrochemiluminescence Featuring High Efficiency, High Stability, and Ultrasensitive Ion Detection Capability.

Herein, for the first time, we introduced a novel electrochemiluminescence (ECL) luminophore based on a one-dimensional g-CN nanotube using KSO as the coreactant. The g-CN nanotube/KSO couple displayed very satisfactory ECL performance, i.e.

Skin-Inspired Hair-Epidermis-Dermis Hierarchical Structures for Electronic Skin Sensors with High Sensitivity over a Wide Linear Range.

The quest for both high sensitivity and a wide linear range in electronic skin design is perpetual; unfortunately, these two key parameters are generally mutually exclusive. Although limited success in attaining both high sensitivity and a wide linear range has been achieved material-specific or complicated structure design, addressing the conflict between these parameters remains a critical challenge. Here, inspired by the human somatosensory system, we propose hair-epidermis-dermis hierarchical structures based on a reduced graphene oxide/poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) aerogel to reconcile this contradiction between high sensitivity and a wide linear range.

Adsorption and desorption mechanisms on graphene oxide nanosheets: Kinetics and tuning.

A knowledge of the adsorption and desorption behavior of sorbates on surface adsorptive site (SAS) is the key to optimizing the chemical reactivity of catalysts. However, direct identification of the chemical reactivity of SASs is still a challenge due to the limitations of characterization techniques. Here, we present a new pathway to determine the kinetics of adsorption/desorption on SASs of graphene oxide (GO) based on total internal reflectance fluorescence microscopy.

Delayed surgery for localised and metastatic renal cell carcinoma: a systematic review and meta-analysis for the COVID-19 pandemic.

Purpose: The COVID-19 pandemic has led to the cancellation or deferment of many elective cancer surgeries. We performed a systematic review on the oncological effects of delayed surgery for patients with localised or metastatic renal cell carcinoma (RCC) in the targeted therapy (TT) era.

Method: The protocol of this review is registered on PROSPERO(CRD42020190882).