Identification of nine mutant genes and establishment of three prediction models of organ tropism metastases of non-small cell lung cancer.

Background: Most Non-small cell lung cancer (NSCLC) patients tend to have metastases at the initial diagnosis. However, limited knowledge has been established regarding which factors, are associated with its metastases. This study aims to identify more biomarkers associated with its organ tropism metastasis and to establish models for prediction of its metastatic organs.

Suppresses High-Fat Diet-Induced Obesity and Related Metabolic Disorders in Beagles.

Obesity is one of the prevalent chronic diseases in human and companion animals usually associated with several metabolic disorders. The gut commensal bacterium () is known for its therapeutic effects on metabolic disorders and inflammations. Here, we isolated the AKK2 strain from the feces of interferon-inducible protein 204 (IFI204) mice and further evaluated its anti-obesity effects on high-fat diet (HFD)-fed C57BL/6J mice and beagles.

Neoadjuvant Efficacy of Three Targeted Therapy Strategies for HER2-Positive Breast Cancer Based on the Same Chemotherapy Regimen.

(1) Background: The objective of our study was to provide evidence for choosing the optimal neoadjuvant therapy strategies for patients with human epidermal growth factor receptor 2 (HER2)-positive early breast cancer. Three neoadjuvant targeted therapy strategies (H + Py, trastuzumab plus pyrotinib; H, trastuzumab; HP, trastuzumab plus pertuzumab) based on the same chemotherapy regimen (TC, docetaxel and carboplatin) were included in the present study; (2) Methods: We retrospectively analyzed patients with HER2-positive breast cancer who were treated with neoadjuvant TCH + Py, TCH or TCHP, followed by surgery. The outcome was the pathological complete response (pCR) rate; (3) Results: In total, 545 patients were enrolled.

Intracellular bacteriolysis contributes to pathogenicity of by exacerbating AIM2-mediated inflammation and necroptosis.

can survive within phagocytes. Indeed, we confirm in this study that approximately 10% of population persists in macrophages during infection, while the rest are eliminated due to bacteriolysis, which is of particular interest to us. Herein, we observe that the bacteriolysis is an early event accompanied by macrophage death during infection.

Hotspots and trends in ophthalmology in recent 5 years: Bibliometric analysis in 2017-2021.

Purpose: The purpose of this study was to investigate the hotspots and research trends of ophthalmology research.

Method: Ophthalmology research literature published between 2017 and 2021 was obtained in the Web of Science Core Collection database. The bibliometric analysis and network visualization were performed with the VOSviewer and CiteSpace.

Rapid and sensitive analytical strategy for multi-class antibiotic residues analysis in aquatic products with amphiphilic magnetic polymer particles as an effective cleanup adsorbent.

Multi-class antibiotic analysis in aquatic products is still challenging owing to the complex matrix and low co-extraction efficiency of various antibiotics. A facile and sensitive sample pretreatment method based on magnetic dispersive solid-phase extraction was thus developed via systematic optimization of extraction and purification procedure. The multi-class antibiotics can simultaneously be extracted by acetonitrile-2 % acetic acid.

Exploration of prognostic factors and the value of adjuvant chemotherapy in T1a,bN0M0 triple-negative breast cancer: a prospective cohort study based on the SEER database.

Background: There are limited published studies on the prognostic predictors and the value of adjuvant chemotherapy (CT) in T1a,bN0M0 triple-negative breast cancer (TNBC) after local therapy. Therefore, the aim of the present study was to explore the prognostic predictors and the value of adjuvant CT in this population.

Methods: We identified T1a,bN0M0 TNBC cases registered in the Surveillance, Epidemiology, and End Results (SEER) database between 2010 and 2015.

DNA Motifs and an Accessory CRISPR Factor Determine Cas1 Binding and Integration Activity in .

CRISPR-Cas systems empower prokaryotes with adaptive immunity against invasive mobile genetic elements. At the first step of CRISPR immunity adaptation, short DNA fragments from the invaders are integrated into CRISPR arrays at the leader-proximal end. To date, the mechanism of recognition of the leader-proximal end remains largely unknown.

Stereoselective bioaccumulation and dissipation of pyrisoxazole in earthworm-soil microcosm.

Pyrisoxazole is a chiral fungicide with high sterilizing activity to the plant pathogenic bacteria. It has two chiral C atoms, which bring four stereoisomers. The present work was the first time to explore the stereoselective bioaccumulation behavior of pyrisoxazole in earthworms by chiral liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS).

Magnetic polymer particles as a highly efficient and facile cleanup adsorbent for multi-pesticide residues analysis in aquatic products.

A novel and efficient sample pretreatment procedure using magnetic particles was exploited for the determination of multi-pesticide residues in aquatic products. The magnetic adsorbent was prepared using divinyl benzene and N-vinyl pyrrolidone as functional monomers modified on the FeO @SiO. The obtained magnetic adsorbent, octadecylsilane sorbents, and graphitized carbon black were employed as effective adsorbents to remove matrix interferences in aquatic products, and their dosages were optimized.

The lens epithelium as a major determinant in the development, maintenance, and regeneration of the crystalline lens.

The crystalline lens is a transparent and refractive biconvex structure formed by lens epithelial cells (LECs) and lens fibers. Lens opacity, also known as cataracts, is the leading cause of blindness in the world. LECs are the principal cells of lens throughout human life, exhibiting different physiological properties and functions.

Production of Multiple Talaroenamines from via One-Pot/Two-Stage Precursor-Directed Biosynthesis.

Nineteen new talaroenamine derivatives, talaroenamines F1-F19 (-), were isolated from the Yellow River wetland derived HPU-J01 by use of a one-pot/two-stage precursor-directed biosynthesis approach. During this approach, the initial precursor -methylaniline was first used as a carrier to capture the biologically synthesized cyclohexanedione to produce talaroenamine F, and then the other aniline derivatives were employed to replace the -methylaniline fragment of talaroenamine F to generate the final products. LC-MS analysis showed that only four compounds (, , , and ) could be produced by the traditional precursor-directed biosynthesis in which the aniline precursors were added simultaneously.

Functional characterization of MEKK3 in the intestinal immune response to bacterial challenges in grass carp ().

Mitogen-activated protein kinase kinase kinase 3 (MEKK3) is an evolutionarily conserved Ser/Thr protein kinase of the MEKK family that is essential for the host immune response to pathogen challenges in mammals. However, the immune function of MEKK3s in lower vertebrate species, especially in bony fish, remains largely unknown. In this study, a fish MEKK3 (designated MEKK3) gene was cloned and identified from grass carp ().

Trust in government buffers the negative effect of rumor exposure on people's emotions.

Unlabelled: Although we are surrounded by various kinds of rumors during the coronavirus disease pandemic, little is known about their primary content, what effect they might have on our emotions, and the potential factors that may buffer their effect. Combining qualitative (study 1 extracted 1907 rumors from top rumor-refuting websites using the Python Web Crawler and conducted content analysis) and quantitative (study 2 conducted an online survey adopting a three-wave design,  = 444) research methods, the current study revealed that government-related rumors accounted for the largest proportion of rumors during the outbreak stage of the pandemic and were positively associated with the public's negative emotions. We also found that trust in government negatively moderated the relationship between government-related rumors and negative emotions.

Bioinformatic analysis of the role of solute carrier-glutamine transporters in breast cancer.

Background: Breast cancer (BC) is a highly heterogeneous disease. Solute carriers (SLCs) have been involved in the tumor progression of various cancer types. This study aimed to evaluate the role of these SLC-related glutamine transporters in the prognosis of BC patients by bioinformatics analysis.

A DLSTM-Network-Based Approach for Mechanical Remaining Useful Life Prediction.

Remaining useful life prediction is one of the essential processes for machine system prognostics and health management. Although there are many new approaches based on deep learning for remaining useful life prediction emerging in recent years, these methods still have the following weaknesses: (1) The correlation between the information collected by each sensor and the remaining useful life of the machinery is not sufficiently considered. (2) The accuracy of deep learning algorithms for remaining useful life prediction is low due to the high noise, over-dimensionality, and non-linear signals generated during the operation of complex systems.

Rational highly dispersed ruthenium for reductive catalytic fractionation of lignocellulose.

Producing monomeric phenols from lignin biopolymer depolymerization in a detachable and efficient manner comes under the spotlight on the fullest utilization of sustainable lignocellulosic biomass. Here, we report a low-loaded and highly dispersed Ru anchored on a chitosan-derived N-doped carbon catalyst (RuN/ZnO/C), which exhibits outstanding performance in the reductive catalytic fractionation of lignocellulose. Nearly theoretical maximum yields of phenolic monomers from lignin are achieved, corresponding to TON as 431 mol mol, 20 times higher than that from commercial Ru/C catalyst; high selectivity toward propyl end-chained guaiacol and syringol allow them to be readily purified.

Impact of Marital Status on Prognosis of Patients With Invasive Breast Cancer: A Population-Based Study Using SEER Database.

Objective: This study aimed to investigate the prognostic roles of marital status in patients with invasive breast cancer. Method: We extracted the data of patients with invasive breast cancer who were diagnosed during 2010-2015 and had complete staging and molecular typing from the Surveillance, Epidemiology, and End Results (SEER)-18 database. Kaplan-Meier curve method and Cox regression analysis were performed to investigate the differences in breast cancer-specific survival (BCSS) and overall survival (OS) in the total population and various subgroups with different marital statuses.

Towards Evaluating Pitch-Related Phonation Function in Speech Communication Using High-Density Surface Electromyography.

Pitch, as a sensation of the sound frequency, is a crucial attribute toward constructing a natural voice for communication. Producing intelligible sounds with normal pitches depend on substantive interdependencies among facial and neck muscles. Clarifying the interrelations between the pitches and the corresponding muscular activities would be helpful for evaluating the pitch-related phonating functions, which would play a significant role both in training pronunciation and in assessing dysphonia.

Targeting HECTD3-IKKα axis inhibits inflammation-related metastasis.

Metastasis is the leading cause of cancer-related death. The interactions between circulating tumor cells and endothelial adhesion molecules in distant organs is a key step during extravasation in hematogenous metastasis. Surgery is a common intervention for most primary solid tumors.

Body mass index and serum markers associated with progression-free survival in lung cancer patients treated with immune checkpoint inhibitors.

Background: ICIs have remarkably affected the treatment strategies for numerous malignancies, including lung cancer. However, only a fraction of patients experience durable responses to ICIs; thus, there is an urgent need to identify the parameters related to ICI therapeutic effects. In this study, we investigated nutritional status surrogates and several serum markers to estimate the efficacy of ICIs.

Bacillus coagulans in Combination with Chitooligosaccharides Regulates Gut Microbiota and Ameliorates the DSS-Induced Colitis in Mice.

Ulcerative colitis (UC) are chronic inflammatory disorders, which may be caused by intestinal barrier dysfunction, immune system disorders and intestinal microbiota dysbiosis. Synbiotic, the combination of probiotics and prebiotics, is thought to be a pragmatic approach in mitigating inflammation in UC. Bacillus coagulans has been recognized as a potential probiotic for treating intestinal diseases because of its favorable industrial and probiotic properties, including sporulation and lactic acid production.

Exploration of the breast ductal carcinoma in situ signature and its prognostic implications.

Following the implementation of breast screening programs, the occurrence of ductal carcinoma in situ (DCIS) as an early type of neoplasia has increased. Although the prognosis is promising, 20%-50% of DCIS patients will progress to invasive ductal carcinoma (IDC) if not treated. It is essential to look for promising biomarkers for predicting DCIS prognosis.