Pilose antler (Cervus elaphus Linnaeus) polysaccharide and polypeptide extract inhibits bone resorption in high turnover type osteoporosis by stimulating the MAKP and MMP-9 signaling pathways.

Ethnopharmacological Relevance: Pilose antler is a traditional Chinese medicine used to improve kidney function, strengthen tendons and bones, and prolong life, among other uses. It is widely employed in the treatment of osteoporosis. However, the molecular mechanisms underlying the treatment of high turnover osteoporosis are not fully understood.

Trichosanthis Pericarpium Aqueous Extract Protects H9c2 Cardiomyocytes from Hypoxia/Reoxygenation Injury by Regulating PI3K/Akt/NO Pathway.

Trichosanthis Pericarpium (TP) is a traditional Chinese medicine for treating cardiovascular diseases. In this study, we investigated the effects of TP aqueous extract (TPAE) on hypoxia/reoxygenation (H/R) induced injury in H9c2 cardiomyocytes and explored the underlying mechanisms. H9c2 cells were cultured under the hypoxia condition induced by sodium hydrosulfite for 30 min and reoxygenated for 4 h.

[Qualitative and quantitative detection of Poria cocos by near infrared reflectance spectroscopy].

Objective: The present study is concerning qualitative and quantitative detection of Poria cocos quality based on FT-near infrared (FT-NIR) spectroscopy combined with chemometrics.

Method: The Poria cocos polysaccharides contents were determined by UV. Transmission mode was used in the collection of NIR spectral samples.

[Changes of fingerprints with crude and processed cassiae semen in xuezhiling tablets].

Objective: To establish an effective and convenient method for HPLC fingerprints of Xuezhiling tablets and new Xuezhiling tablets, and to observe the changes of fingerprint with crude and processed Cassiae Semen in Xuezhiling tablets.

Methods: The HPLC with Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) column was used for the gradient elution of acetonitrile-0.

Simultaneous determination and pharmacokinetic comparisons of multi-ingredients after oral administration of radix salviae miltiorrhizae extract, hawthorn extract, and a combination of both extracts to rats.

A simple and sensitive HPLC method was developed for simultaneous determination of danshensu (DSS), rosmarinic acid (RA), lithospermic acid (LA), salvianolic acid B (SAB), and hyperoside (HP) in rat plasma. This method validated was successfully applied to the pharmacokinetic study of the main active ingredients after oral administration of Radix Salviae Miltiorrhizae extract (SME), hawthorn extract (HTE), and a combination of both extracts (2.5 : 1) to rats.

Glucuronidation of aurantio-obtusin: identification of human UDP-glucuronosyltransferases and species differences.

1. The aurantio-obtusin's glucuronide was detected when aurantio-obtusin was incubated with human liver microsomes (HLMs). Recombinant UGT isoforms screening experiment showed that UGT1A8 was the major isoform contributed to the glucuronidation.

Comparative pharmacokinetics of baicalin, wogonoside, baicalein and wogonin in plasma after oral administration of pure baicalin, radix scutellariae and scutellariae-paeoniae couple extracts in normal and ulcerative colitis rats.

The aim of this study was to investigate the pharmacokinetic profiles of baicalin, wogonoside, baicalein and wogonin after oral administration of pure baicalin, Radix scutellariae and Scutellariae-Paeoniae couple extracts were administered and the pharmacokinetics profiles were compared between normal and ulcerative colitis rats. The plasma concentrations of the four flavonoids were determined by using a simple and rapid high-performance liquid chromatography method. All the rats were divided randomly into two groups (ulcerative colitis and normal groups).

HPLC method determination of isoliquiritin apioside and isoliquiritin in rat plasma for application in pharmacokinetic study after an oral administration of zhigancao extract.

A sensitive HPLC method was developed for the quantitative determination of isoliquiritin apioside (ILA) and isoliquiritin (IL) in rat plasma. After protein precipitation with acetonitrile, chloroform was used to separate lipid-soluble impurities from the plasma samples and remove acetonitrile. A chromatography was carried out on Diamonsil C18 (150 × 4.

[Study on the tissue distribution of berberine from Rhizoma Coptidis and compatibility with Rhizoma Coptidis and Cortex Cinnamomi in rats].

Objective: To study the effects of Rhizoma Coptidis extracts and Rhizoma Coptidis and Cortex Cinnamomi extracts on the distribution of terberine in rats' tissues.

Methods: The rats were intragastrically given the two different extracts. HPLC was used to determine the concentration of berberine in rats' tissues and the sample was extracted by liquid-liquid extraction method.

[Studies on quality evaluation of Penis et Testis Cervi by Grey incidence degree method].

Objective: To systematically evaluate the quality of Penis et Testis Cervi.

Methods: Grey model was set up by determining six main compositions contained in the samples.

Results: The quality evaluation on 9 samples by this model was as same as tradition and reality.

[Determination of oridonin and rosemarinic acid in Rabdosia rubescens by HPLC].

Objective: To establish the method for determining the content of oridonin and rosemarinic acid in Rabdosia rubescens by HPLC.

Methods: Using ODS-C18 column (150 mm x 4.6 mm, 5 microm), methanol-0.

FloatBoost learning and statistical face detection.

A novel learning procedure, called FloatBoost, is proposed for learning a boosted classifier for achieving the minimum error rate. FloatBoost learning uses a backtrack mechanism after each iteration of AdaBoost learning to minimize the error rate directly, rather than minimizing an exponential function of the margin as in the traditional AdaBoost algorithms. A second contribution of the paper is a novel statistical model for learning best weak classifiers using a stagewise approximation of the posterior probability.