Discovery and Characterization of Intercondylar Transphyseal Complexes and their Oncological Significance in Transphyseal Extension of Pediatric Osteosarcoma.

Objective: To explore whether there exist undiscovered transphyseal vasculature-canal compound structures in immature femurs and tibias, and reveal their potential oncological impact.

Methods: This investigation was divided into a morphological study and a clinical study. In the morphological part, a new-identified anatomic structure was investigated by using radiographical, anatomical, and histological methodologies.

Efficacy of neuroendoscopic surgery versus craniotomy for supratentorial hypertensive intracerebral hemorrhage: A meta-analysis of randomized controlled trials.

Background: Hypertensive cerebral hemorrhage (HCH) is a potentially life-threatening neurological condition with an extremely high morbidity and mortality. In recent years, neuroendoscopy has been used to treat intracerebral hemorrhage (ICH). However, the choice of neuroendoscopic surgery versus craniotomy for patients with intracerebral hemorrhages is controversial.

Regulatory effects of insulin-like growth factor-1 on the expression of sensory neuropeptide mRNAs in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate.

Objective: To determine the effects of insulin-like growth factor-1 (IGF-1) on the expression of preprotachykinin (PPT) mRNA encoding substance P (SP) and calcitonin gene-related peptide (CGRP) mRNA in cultured dorsal root ganglion (DRG) neurons with excitotoxicity induced by glutamate (Glu).

Methods: DRGs were dissected from embryonic day 15 Wistar rats. DRG neurons were dissociated and cultured for 48 h and then exposed to Glu (0.

[Comparison of Cj1136, Cj1138 and Cj1139 genes among Campylobacter jejuni strains].

Objective: By sequenceing the Cj1136, Cj1138 and Cj1139 gene of Campylobacter jejuni (C. jejuni) strains associated with Guillain-Barré Syndrome (GBS), features of Cj1136, Cj1138 and Cj1139 gene were studied. Results were compared with the C.

[Effect of omega-3 long-chain polyunsaturated fatty acids on the cultured oligodendrocyte precursor cells].

Objective: To explore the effect of long-chain polyunsaturated fatty acids (LPFA) on the survival and process growth of the brain hippocampal Oligodendrocyte precursor cells (OPC).

Method: Two kinds of cultured neural progenitor cells isolated from adult rat hippocampus were used. After arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) treatment respectively, the activity of cells were determined by Lactate dehydrogenase (LDH) assay, and the quantitative measurements of the cell processes were done after the fluorescent immune cells staining.

Exogenous galanin regulates capsaicin-evoked substance P release from primary cultured dorsal root ganglion neurons.

Objectives: To determine the effect of exogenous galanin on capsaicin-evoked substance P (SP) release from primary cultured embryonic rat dorsal root ganglion (DRG) neurons.

Methods: DRG was dissected out from embryonic 15-day-old Wistar rat and cultured as dissociated cells for 2 days then exposed to galanin (1 nmol/L, 10 nmol/L, 100 nmol/L). After 4 days incubation with exogenous galanin, the levels of mRNAs for SP and vanilloid receptor 1 (VR1) and protein for VR1 were estimated by RT-PCR and Western blot, respectively.

Regulatory effect of nerve growth factor on release of substance P in cultured dorsal root ganglion neurons of rat.

Objective: To investigate the regulatory effects of nerve growth factor (NGF) on basal and capsaicin-induced release of neuropeptide substance P (SP) in primary cultured embryonic rat dorsal root ganglion (DRG) neurons.

Methods: DRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF (10 ng/mL, 30 ng/mL, or 100 ng/mL) for 72 h.

[Change of hippocampal NMDA receptor and emotional behavior and spatial learning and memory in status epilepticus rat model].

Aim: SD rats were utilized for the purpose of the exploration of effects of status epilepticus (SE) on their emotional behavior, spatial learning and memory, and explorating its molecular mechanism.

Methods: Forty maturity male SD rats, weighing (200 +/- 20) g were divided randomly and equally into SE group (SG) and normal control group (NG). The SG rats were induced by Pentylenetetrazole (PTZ) and the control animals received a saline (0.

Butyrate sensitizes the release of substance P and calcitonin gene-related peptide evoked by capsaicin from primary cultured rat dorsal root ganglion neurons.

Objectives: To investigate whether butyrate increases substance P (SP) and calcitonin gene-related peptide (CGRP) release evoked by capsaicin from primary cultured dorsal root ganglion (DRG) neurons.

Methods: DRG was dissected out from embryonic 15-day-old Wistar rat and cultured as dissociate cells for 24 h then exposed to butyrate (0.01 mmol/L, 0.

[Spectrophotometric determination of chondroitin sulfate with victory blue B].

In pH 4.0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value is linear with chondroitin sulfate concentration in the range of 0.

[Resonance-scattering spectral determination of H2O2 using rhodamine 6G association particles].

Under the conditions of 0.02 mol x L(-1) HCl-4.0 x 10(-4) mol x L(-1) KI-1.