Effects of Xinjiaxiangruyin on the TLR7 pathway in influenza virus-infected lungs of mice housed in a hygrothermal environment.

Background: To investigate the effects and immunological mechanisms of the traditional Chinese medicine Xinjiaxiangruyin on controlling influenza virus (FM1 strain) infection in mice housed in a hygrothermal environment.

Methods: Mice were housed in normal and hygrothermal environments, and intranasally infected with influenza virus (FM1). A high-performance liquid chromatography fingerprint of Xinjiaxiangruyin was used to provide an analytical method for quality control.

Injured liver-released miRNA-122 elicits acute pulmonary inflammation via activating alveolar macrophage TLR7 signaling pathway.

Hepatic injury is often accompanied by pulmonary inflammation and tissue damage, but the underlying mechanism is not fully elucidated. Here we identify hepatic miR-122 as a mediator of pulmonary inflammation induced by various liver injuries. Analyses of acute and chronic liver injury mouse models confirm that liver dysfunction can cause pulmonary inflammation and tissue damage.

Anti-influenza activity of berberine improves prognosis by reducing viral replication in mice.

Berberine, a natural isoquinoline alkaloid isolated from the berberis species, has a wide array of biological properties such as anti-inflammatory, antibacterial, antifungal, and antihelminthic effects. We evaluated the antiviral effect of berberine against influenza A/FM1/1/47 (H1N1) in vivo and in vitro. The results showed that berberine strongly suppressed viral replication in A549 cells and in mouse lungs.

Effects of different principles of Traditional Chinese Medicine treatment on TLR7/NF-κB signaling pathway in influenza virus infected mice.

Background: Influenza virus is a single-stranded RNA virus that causes influenza in humans and animals. About 600 million people around the world suffer from influenza every year. Upon recognizing viral RNA molecules, TLR7 (Toll-like receptor) initiates corresponding immune responses.

Effects of Gui Zhi Ma Huang Ge Ban Tang on the TLR7 Pathway in Influenza Virus Infected Mouse Lungs in a Cold Environment.

Objective: We wished to investigate the effects of the traditional Chinese medicine Gui Zhi Ma Huang Ge Ban Tang on controlling influenza A virus (IAV) infection and improving inflammation in mouse lungs.

Method: Mice were maintained in normal and cold environments and infected with IAV by intranasal application, respectively. Real-time quantitative polymerase chain reaction was used to measure mRNA expression of TLR7, myeloid differentiation primary response 88 (MyD88), and nuclear factor-kappa B (NF-B)p65 in the TLR7 signaling pathway and virus replication in lungs.

Hygrothermal environment may cause influenza pandemics through immune suppression.

Over the past few decades, climate warming has caused profound changes in our living environment, and human diseases, including infectious diseases, have also been influenced by these changes. However, it remains unclear if a warm-wet climate can influence the infectivity of influenza and result in influenza pandemics. This study focused on observations of how the hydrothermal environment influences the infectivity of the influenza virus and the resulting immunoreactions of the infected mice.

Dysbiosis of gut microbiota induced the disorder of helper T cells in influenza virus-infected mice.

It is widely understood that commensal microbiota contributes to the maintenance of intestinal homeostasis through dynamic interactions with a body's immunity. And the immune regulation is important for the influenza vaccine's effectiveness after body injection, however, the mechanism between commensal microbiota and vaccine's effectiveness remains unknown. The impact that individual bacteria species have on the balance of the systemic immune system beyond the local intestinal mucosal tissues also remains less clear, and the related mechanism is still unknown.

Andrographolide as an anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway.

Objective: To observe the anti-virus effects of andrographolide (AD) on the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) signaling pathway when immunological cells were infected with H1N1.

Methods: Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation, and immunological cells were harvested after cytokines stimulation. Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line (16HBE).

A novel reporter system for neutralizing and enhancing antibody assay against dengue virus.

Background: Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE).

Microbiota regulates the TLR7 signaling pathway against respiratory tract influenza A virus infection.

Although intestinal flora are crucial in maintaining immune homeostasis of the intestine, the role of intestinal flora in immune responses at other mucosal surfaces remains less clear. Here, we show that intestinal flora composition critically regulates the toll-like receptor 7 (TLR7) signaling pathway following respiratory influenza virus infection. TLR7 ligands rescued the immune impairment in antibiotic-treated mice.

Identification and characterization of a linearized B-cell epitope on the pr protein of dengue virus.

The four serotypes of dengue virus (DENV) represent one of the major mosquito-borne pathogens globally; so far no vaccine or specific antiviral is available. During virion maturation, the pr protein is cleaved from its precursor form the prM protein on the surface of immature DENV by host protease. Recent findings have demonstrated that the pr protein not only played critical roles in virion assembly and maturation, but was also involved in antibody-dependent enhancement of DENV infection.

Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway.

The antivirus effect of quercetin and oseltamivir on the Toll-like receptor 7 (TLR7) signaling pathway was observed when dendritic cells and macrophages were infected with H1N1. Leukomonocytes were obtained from umbilical cord blood and harvested after stimulation by recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (rhGM-CSF) and recombinant human Interleukin 4 (rhIL-4). Virus-infected cell model was established by human bronchial epithelial cells (16HBE) infected with H1N1.

[The immunomodulatory effects of sijunzi decoction and its disassembled prescription on D-galactose-induced aging mice].

Objective: To observe the immunomodulatory effects of codonopsis, atractylodes macrocephala, tuceahoe, broiled licorice and sijunzi decoction on D-galactose-induced aging mice.

Methods: The models of aging mice were induced by D-galactose, the garlands and splenic lymphocyte transformation test (MTT) were used to determine the ability of erythrocytes immune and lymphocytes conversion; The content of maleic dialdehyde (MDA), activity of superoxide dismutase (SOD) and glutathione pemfidase (GSH-Px) in serum were detected.

Results: Sijunzi decoction and its disassembled prescription codonopsis could significantly increase the ability of T lymphocyte transformation (P<0.

[Effects of curcumin on TNF-alpha and TGF-beta1 in serum and lung tissue of SiO2-induced fibrosis in mice].

Aim: To study the effects of curcumin on TNF-alpha and TGF-beta1 in serum and lung tissue of SiO2-induced fibrosis in mice.

Methods: 75 mice were divided into five groups. After treated with curcumin 6 and 9 mice of each group were sacrificed on day 14 and 42 respectively to take their blood and lung tissue.

[Studies on antiproliferative effect of flavones compounds isolated from Yao herb medicines].

Objective: To detect the cytotoxicity and in vitro antiproliferative effects of the flavones compounds isolated from some Yao herbal medicines, and analyze the basic structure-activity relationship.

Methods: The cytotoxicity on normal cells and antiproliferative effect on tumor cells were tested by MTT reduction assay respectively.

Results: Among the 6 flavones, the Eriodictyol-7-O-a-D-glucoside, Naringenin-7-O-beta-D-glucoside, quercetin and quercetin-3-O-beta-D-glucoside showed very low cytotoxicity to the two normal cells, Vero and MC cells, while the naringenin was high toxic to both of them.

[Clinical value of the detection of hepatitis C virus core antigen].

Aim: To explore the value of detecting hepatitis C virus core antigen in HCV infected diseases.

Methods: The HCV-RNA was tested by fluorescent quantitative polymerase chain reaction (FQ-PCR). The core antigens of HCV and anti-HCV were tested by enzyme-linked immunosorbent assay (ELISA).

[Preventive and therapeutic effects of qiongyugao on hepatocellular carcinoma via inhibition of the expression of HBxAg in hepatic carcinoma cells].

Aim: To investigate the effects of qiongyugao on the expression of hepatitis B x antigen (HBxAg) in BALB/c-nu mice into which human hepatic carcinoma cells were transplanted, and to analyze its specific mechanism in prophylaxis and treatment of hepatocellular carcinoma.

Methods: A nude mouse model with the transplantation of human hepatic carcinoma cells was established to observe the preventive and therapeutic effects of qiongyugao on the body weight and tumor weight of the mice. The expression of HBxAg in tumor and liver tissue wsa detected by immunohistochemical studies.

[Effects of Dengue virus infection on PGIS expression and PGI2 secretion of human vascular endothelial cells].

Aim: To elucidate the effects of Dengue virus on the expression and secretion of prostacyclin (PGI(2)) in endothelial cells.

Methods: Cultured human umbilical vein endothelial cells (HUVEC) were infected by Dengue virus II(DV(2)) for different duration. PGI(2) level in the supernatant was determined by radioimmune assay.

[Effect of IL-6 and TNF-alpha on Dengue virus infection of human dendritic cells].

Aim: To study the effect of IL-6 and TNF-alpha on Dengue virus (DV) infection of human dendritic cells (DCs).

Methods: Monocytes isolated from healthy human peripheral blood were incubated in medium with GM-CSF and IL-4 for 7-10 days, and then were collected and identified by transmission electron microscope, immunohistochemistry and lymphocytes stimulatory ability assay. DCs infected with DV type II (DV(2)) were incubated with IL-6, TNF-alpha at high, medium and low concentration.

[Expression of tissue factor induced by IL-6 in HUVEC].

Objective: To investigate the effects of IL-6 at the expression of tissue factor (TF) in human vein endothelial cells(HUVECs).

Methods: HUVECs were incubated with IL-6 at the concentration of 0.5 ng/mL.

[Study on dengue virus infection of human dendritic cells].

To study Dengue virus (DV) infection of human dendritic cells (DC). Monocyte isolated from healthy human peripheral blood were incubated in medium with GM-CSF and IL-4 for more than 7 days. DCs were then collected and identified by transmission electron microscope, immunohistochemistry and lymphocytes stimulatory ability.

Analysis of clinical effect of high-intensity focused ultrasound on liver cancer.

Aim: To evaluate the clinical effect of high-intensity focused ultrasound (HIFU) in the treatment of patients with liver cancer.

Methods: HIFU treatment was performed in 100 patients with liver cancer under general anesthesia and by a targeted ultrasound. Evaluation of efficacy was made on the basis of clinical symptoms, liver function tests, AFP, MRI or CT before and after the treatment.