[Construction and expression of human EpCAM eukaryotic expression vectors and identification of their products].

Aim: To construct EpCAM eukaryotic expression vectors pIg-EpCAM and pEGFP-EpCAM and to express them in COS7 cells.

Methods: EpCAM cDNA was amplified by PCR and then inserted into the pIg and pEGFP vectors respectively to construct recombinant vectors pIg-EpCAM and pEGFP-EpCAM. The two recombinant vectors were transfected into COS7 cells under the mediation of liposome.

[Comparative studies on the resistance of hepatitis B by Syringa extract, IFN and ganyanling in HepG2.2.15 cells].

Aim: To explore the effects of Syringa extract, IFN and ganyanling on the the survival rate of HepG2.2.15 cells and the secretion of HBsAg and HBeAg by HepG2.

[Study on cellular and serum concentration of calcium and magnesium in peripheral blood cells of cirrhosis].

Objectives: To study on the changes of intracellular calcium and magnesium in cirrhosis and its clinical significance.

Methods: The calcium and magnesium were determined in serum (SCa, SMg), platelets (PCa, PMg), mononuclear cells (MNCCa, MNCMg), polymorphonuclear cells (PMNCa, PMNMg) and erythrocytes (RCa, RMg) of 50 patients with uncompensative cirrhosis (group A) and of 35 patients with compensative cirrhosis (group B). 35 health persons were the control group.