[Methodological study on magnetic affinity immunoassay for detecting chloramphenicol].

Aim: To establish a sensitive immunoassay for detection chloramphenicol using magnetic beads as solid phase.

Methods: This assay employs the competitive inhibition, the FITC conjugated with anti-chloramphenicol monoclonal antibodies and the alkaline phosphatase conjugated with chloramphenicol respectively. Magnetic beads were coupled with sheep anti-FITC antibodies as solid phase.

[Application of magnetic particle antibody immunoassay in detection of anti-Schistosoma japonicum egg antibody].

Objective: To establish a magnetic particle antibody immunoassay (MPAIA) for the detection of specific antibody in sera of schistosomiasis patients.

Methods: Fluorescein isothiocyanate (FITC) was used to label Schistosoma japonicum soluble egg antigen (Sj-SEA). Anti-human IgG coated with alkaline phosphatase (ALP) as enzyme-labeled second antibody, and magnetic beads were coupled with sheep anti-FITC antibody as solid phase.

[Establishment of the magnetic separation enzyme immunofluorescence method for detecting insulin].

Aim: To establish a convenient and sensitive magnetic separation enzyme immunofluorescence (MEIF) method for detecting human insulin.

Methods: Two monoclonal antibodies (mAbs) were conjugated with FITC and alkaline phosphatase (AP) respectively, which were incorporated magnetic solid phase separation. Magnetic beads were coupled with sheep anti-FITC antibody as solid phase and 4-Methylumbelliferyl-phosphoric acid (4-MUP) was used as substrate to set up MElF for detecting insulin.

[Establishment of chemiluminescence immunoassay for detecting Staphylococcal enterotoxin B and C1].

Aim: To establish chemiluminescence immunoassay (CLIA) for detecting staphylococcal enterotoxin B (SEB) and C1 (SEC1) and compare its sensitivity and stability with ELISA.

Methods: The anti-SEB and SEC1 monoclonal antibodies (mAb) were purified by Q Sepharose Fast Flow chromatographic column. The alkaline phosphatase (AP) conjugated mAbs FMMU-SEB.

[Methodological study on magnetic enzymic immunoassay for detecting free hCGbeta subunit].

Aim: To establish a novel magnetic enzyme immunoassay (MEIA) for detecting human choriogonadotropin free beta subunit (hCGbeta).

Methods: Two monoclonal antibodies (mAb) were used for conjugating with FITC and with alkaline phosphatase(AP) respectively, which incorporated magnetic solid phase separation. Magnetic beads were coupled with sheep anti-FITC antibody as solid phase, and phenolphthalein monophosphate was used as substrate to set up MEIA for detecting hCGbeta.