Integrative Analysis of Minichromosome Maintenance Proteins and Their Prognostic Significance in Melanoma.

Background: Minichromosome maintenance () is known for participating in cell cycle progression, as well as DNA replication. While the diverse expression patterns and prognostic values of s in melanoma still remained unclear.

Methods: In the present study, the transcriptional and clinical profiles of s were explored in patients with melanoma from multiple databases, including GEO, TCGA, ONCOMINE, GEPIA, UALCAN, cBioPortal, and TIMER databases.

SphK1-targeted miR-6784 inhibits functions of skin squamous cell carcinoma cells.

Sphingosine kinase 1 (SphK1) is overexpressed in skin squamous cell carcinoma (SCC). It has emerged as a novel therapeutic oncotarget. The current study identified a novel SphK1-targeting microRNA, microRNA-6784 (miR-6784).

virB11 gene potentially involves in ATP metabolism to provide energy in H. pylori infection.

Helicobater pylori (H. pylori) is the most important bacteria known to be associated with various gastroduodenal diseases. virB11 gene is a structural gene of tfs3a genes cluster in the plasticity region of H.

miRNA-221 promotes cutaneous squamous cell carcinoma progression by targeting PTEN.

Background: Cutaneous squamous cell carcinoma (CSCC) is a common type of skin malignancy. MicroRNA-221 (miRNA-221) is a critical non-coding RNA in tumor initiation and progression. However, the molecular mechanisms of miRNA-221 in the development of CSCC remain unknown.

Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns.

Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly detected during mass screening for neonatal disease. We developed a method to measure reduced glutathione (GSH) and glutathione disulfide (GSSG) using tandem mass spectrometry (MS/MS) for detecting G6PD deficiency.

Methods: The concentration of GSH and the GSH/GSSG ratio in newborn dry-blood-spot (DBS) screening and in blood plus sodium citrate for test confirmation were examined by MS/MS using labeled glycine as an internal standard.

Association of plasminogen activator inhibitor-1 and vitamin D receptor expression with the risk of keloid disease in a Chinese population.

Keloid disease (KD) is a benign fibroproliferative scarring condition of unknown etiopathogenesis. Plasminogen activator inhibitor-1 (PAI-1) and vitamin D receptor (VDR) have been shown to play important roles in the progression of tissue fibrosis; therefore, both these genes are potential susceptibility genes for KD. We aimed to determine whether the gene expression levels of PAI-1 and VDR are altered in Chinese KD patients.

Cloning of mating-type gene MAT1-1 from the caterpillar medicinal mushroom, Cordyceps militaris (Ascomycetes) using TAIL-PCR technology.

Cordyceps militaris and Ophiocordyceps sinensis (syn. Cordyceps sinensis), 2 well-known traditional Chinese medicines, contain the same bioactive components and share a similar developmental process. In this study, one C.

[Blood spot carnitine and acylcarnitine in newborn to adolescence: measured by tandem mass spectrometry].

Objective: To determine the levels of blood spot carnitine and acylcarnitine in children aged 0-15 years by tandem mass spectrometry, offer basic data for evaluating carnitine nutritional status and diagnosing metabolic diseases of organic acid and fatty acid.

Methods: The concentration of carnitine and acylcarnitines were measured in blood spot by tandem mass spectrometry using underivatized samples. The samples included those from 1376 perinatal neonates, 49 neonates above 1 week of life, 64 children aged up to 1 year and 401 children aged 1 year to 15 years.

[Value of ultrasonography in the diagnosis and differential diagnosis of testicular tumor].

Objective: To evaluate ultrasonography in the diagnosis and differential diagnosis of testicular tumor.

Methods: Ultrasound findings and post-operation pathological results were retrospectively studied in 172 men with testicular mass 1998 to 2005.

Results: Of the total number, 50 cases were testicular hematoma, 13 testicular cyst, 26 testicular inflammatory node, 25 testicular tuberculosis and 58 testicular tumor.

[Omission factors in detecting early stage prostate cancer by TRUS needle biopsy].

Objective: To analyze and reduce the omission factors in detecting early stage prostate cancer by TRUS needle biopsy, and to improve the diagnosis of the disease.

Methods: A total of 80 benign prostatic hyperplasia patients suspected of prostatic carcinoma underwent TRUS sextant biopsies. The pathological results being negative, the patients received transurethral resection of the prostate (TURP).