Mitochondrial DNA B Resour
October 2018
We sequenced and characterized the complete mitochondrial genome of golden yellow snakehead fish, The mitogenomes contained the typical complement of 13 protein-coding genes, 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and a non-coding control region. They share the same gene arrangement pattern that was identical with most vertebrates. The entire mitochondrial DNA molecule of golden yellow snakehead fish was 16,558 bp long.
View Article and Find Full Text PDFMitochondrial DNA B Resour
July 2018
We sequenced and characterized the complete mitochondrial genome from normal colour (grey black) and mutant colour (orangey red) of . Both mitogenomes contained the typical complement of 13 protein-coding genes, 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and a non-coding control region. They share the same gene arrangement pattern that was identical with most vertebrates.
View Article and Find Full Text PDFOur recent study observed that the expression of Musashi-2 (MSI2), a member of the Musashi family, was up-regulated in hepatitis B virus (HBV) related hepatocellular carcinoma parenchymal cells. Using quantitative PCR, tissue microarray (TMA) and immunohistochemical staining, we evaluated MSI2 mRNA and protein levels in tumor tissues from patients with different stages of hepatocellular carcinoma with paired adjacent noncancerous sample sets. The following techniques were used to further investigate MSI2 function and its potential molecular mechanism: RNAi, wound healing assay, Transwell assay, quantitative PCR and western blot analysis.
View Article and Find Full Text PDFPurpose: Altered expression of serine protease inhibitor peptidase inhibitor clade E member 2 (SERPINE2) associates with human cancer development and progression; thus, this study investigated SERPINE2 expression in gastric cancer tissues for association with clinicopathological and survival data from the patients and then investigated the role of SERPINE2 in gastric cancer cells in vitro.
Methods: The levels of SERPINE2 mRNA in 243 gastric cancer tissues and paired non-cancerous mucosa were determined using quantitative PCR. Inhibition of SERPINE2 expression by small interfering RNA (siRNA) was detected by Western blotting.
Cip1-interacting zinc-finger protein1 (Ciz1) is a nuclear matrix protein associated DNA replication factor which has been implicated in breast and lung cancer progression. However, the clinical significance of Ciz1 expression in colon cancer has not been determined. This study aimed to examine Ciz1 expression pattern and its potential as a biomarker of prognosis in colon cancer.
View Article and Find Full Text PDFIn this study, a strategy of the construction of leaky strains for the extracellular production of target proteins was exploited, in which the genes mrcA, mrcB, pal and lpp (as a control) from Escherichia coli were knocked out by using single- and/or double-gene deletion methods. Then the recombinant strains for the expression of exogenous target proteins including Trx-hPTH (human parathyroid hormone 1-84 coupled with thioredoxin as a fusion partner) and reteplase were reconstructed to test the secretory efficiency of the leaky strains. Finally, the fermentation experiments of the target proteins from these recombinant leaky strains were carried out in basic media (Modified R media) and complex media (Terrific Broth media) in flasks or fermenters.
View Article and Find Full Text PDFBased on the fishery resources data from the bottom trawl surveys conducted on the R/V Beidou in the Yangtze River estuary and its adjacent waters in June, August and October of 2006, the index of relative importance (IRI) was measured to determine the dominant species of fish assemblage, and the niche indicators and their seasonal variations of the dominant species were analyzed. A total of 10 dominant species in the 3 survey cruises were recorded, which were divided into two groups by the Bray-curtis similarity clustering and non-metric multidimensional scaling (MDS) analysis, with a significant seasonal variation of niche breadth and niche overlap. One group included Engraulis japonicus, Champsodon capensis, and Acropoma japonicum, whose niche breadth and niche overlap were larger in summer than in autumn, with a migration from the Yangtze River estuary and its adjacent waters to outer deeper waters, while the other group included Trichiurus haumela, Chaeturichthys stigmatias, Apogon lineatus, Larimichthys polyactis, Psenopsis anomala, Argyrosomus argentatus, and Benthosema pterotum, whose niche breadth and niche overlap were larger in autumn than in summer, with a reverse migration from southern Yellow Sea and northern East China Sea to the Yangtze River estuary and its adjacent waters.
View Article and Find Full Text PDFJ Immunoassay Immunochem
November 2013
To elucidate the significance of Toll-like receptors and their negative regulating factors PPAR-γ and Tollip on the pathogenesis of colitis. Colitis model was induced by TNBS in rat. The expression of TLR2, TLR4, NF-κBp65, PPAR-γ and Tollip was examined by immunohistochemistry (IHC) and reverse-transcription polymerase chain reaction (RT-PCR).
View Article and Find Full Text PDFZhonghua Wei Chang Wai Ke Za Zhi
July 2012
Purpose: Downregulation of metallothionein (MT) genes has been reported in several tumors with discrepant results. This study is to investigate molecular mechanism of MT gene regulation in colon cancer which is characterized by tumor suppressor gene alterations.
Experimental Design: Integral analysis of microarray data with loss of heterozygosity (LOH) information was employed.
Exp Biol Med (Maywood)
November 2011
Biglycan, a member of the small leucine-rich proteoglycan family, has been implicated in the development and progression of human cancers. However, the clinical significance of biglycan expression in gastric cancer has not been determined. In the present study, biglycan mRNA and protein concentrations were analyzed using quantitative realtime reverse transcription polymerase chain reaction and Western blot in 69 gastric cancer and adjacent non-tumorous tissues, respectively.
View Article and Find Full Text PDFJ Immunoassay Immunochem
April 2011
This study elucidates the significance of Toll-like receptor 4 (TLR4), CD14, and nuclear factor (NF)-κB on the pathogenesis of ulcerative colitis (UC). Colonic biopsy specimens were collected from active UC and controls. The expression of TLR4, CD14, and NF-κBp 65 was analyzed by immunohistochemistry (IHC) and reverse-transcription polymerase chain reaction (RT-PCR).
View Article and Find Full Text PDFXi Bao Yu Fen Zi Mian Yi Xue Za Zhi
July 2010
Aim: To investigate the expression of TLR4 and NF-kappaB in ulcerative colitis (UC).
Methods: Colonic biopsy specimens were collected from active UC and controls. The expression of TLR4 and NF-kappaBp 65 were analyzed by immunohistochemistry (IHC) and RT-PCR.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
July 2007
Aim: To explore the possible relationship between expression of PPAR(pevoxisome prodiferator adivated receptor) beta and clinical pathological features of epithelial ovarian carcinoma (EOC).
Methods: PPARbeta expressions in normal ovaries (n=10), ovarian bordline tumors (n=10), and ovarian serous cystadenocarcinomas (n=46) were investigated by immunohistochemistry and RT-PCR.
Results: PPARbeta protein was expressed in normal ovaries and ovarian cancer.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
June 2007
Aim: The differential expression of USP2, USP14 and UBE4A between ovarian serous cystadenocarcinoma and adjacent normal tissues was investigated.
Methods: Restriction fragment differential display polymerase chain reaction (RFDD-PCR), semi-quantitative RT-PCR and immunohistochemical staining were applied to analyze the differentially expressed genes and proteins of ubiquitin specific proteases (USPs), USP2 and USP14, and ubiquitin factor E4A (UBE4A) between ovarian serous cystadenocarcinoma and adjacent normal tissues obtained from 40 patients aged from 29 to 72 years old, collected in 2005 year at excision of surgical operation with ovarian serous cystadenocarcinoma.
Results: USP2, USP14 and UBE4A were over-expressed (>3 folds) in ovarian serous cystadenocarcinoma tissues compared to normal tissues.
Background: Beta-catenin has two distinct roles in E-cadherin mediated cell adhesion and carcinogenesis by activating the wnt/beta-catenin signaling pathway. One occurs at the cell-adhesion site, where cadherins are linked to the actin-based cytoskeleton. The other takes place in the cytoplasm and nuclei and is thought to regulate cell transformation.
View Article and Find Full Text PDFJ Hepatobiliary Pancreat Surg
July 2004
Background/purpose: We aimed to determine the rationality of pericardial devascularization (PCDV) plus proximal splenorenal shunt (PSRS) for cirrhotic patients with portal hypertension with variceal bleeding, using a duplex sonography study of the effects of the different surgical procedures (PCDV, PSRS, and PCDV + PSRS) on the hemodynamics of the portal system.
Methods: Ninety-nine patients with cirrhotic portal hypertension and a history of bleeding esophageal varices were studied. These patients were divided into three groups (PSRS group, PCDV group, and PCDV + PSRS group).