A new method of establishing orthotopic bladder transplantable tumor in mice.

Objective: The present study aims to find a convenient, rapid, and stable method to establish bladder tumor in mice.

Methods: Female Balb/C-nu-nu nude mice (or female T739 mice) were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The stylet of the 24# venous retention needles was bent in a 5° to 7° angle at a distance of 15 mm from the needlepoint to form a circle with 2.

[Expression and clinical significance of nuclear matrix protein 22 and cytokeratin 18 in transitional cell carcinoma of the bladder].

Objective: To evaluate the expression and clinical significance of urinary nuclear matrix protein (NMP22) and cytokeratin 18 (CK18) for transitional cell carcinoma of the bladder.

Methods: Urinary NMP22 and CK18 levels of 293 patients with transitional cell carcinoma of the bladder, 400 patients with non-transitional cell carcinoma of the bladder, and 105 bladder benign disease were analysed by enzyme-linked-immunosorbent assay (ELISA).

Results: The levels of urinary NMP22 and CK18 in the patients with transitional cell carcinoma of the bladder (M = 17.

[Testosterone induces different-featured prostate hyperplasia in castrated and uncastrated mice].

Objective: To study the different features of hyperplasia in castrated and uncastrated mice after testosterone (T) treatment.

Methods: Forty-eight BALB/c mice were randomly divided into 6 groups of 8 in each: castrated (A), uncastrated (B) , castrated + low T (C), uncastrated + low T (D), castrated + high T (E), uncastrated + high T (F). Groups C and D were treated with testosterone solution at the dose of 12.

[Human chorionic gonadotrophin regulates epidermal growth factor in the phallus of hypospadias mice].

Objective: To investigate the etiopathogenesis of congenital hypospadias and the regulating effect of exogenous human chorionic gonadotrophin (hCG) on the epidermal growth factor (EGF) in the phallus of hypospadiac mice.

Methods: Mouse models of congenital hypospadias were established. Fifty healthy male mice randomly selected as normal controls received intraperitoneal injection of normal saline, and another 50 with hypospadias were equally divided into an experimental control group, intraperitoneally injected with 1 ml normal saline, and 4 hCG dose groups treated by hCG intraperitoneal injection at 50 IU, 100 IU, 150 IU and 200 IU respectively for 7 consecutive days.

[The diagnosis and monitoring of transitional cell cancer of the urinary tract through nuclear matrix protein 22].

Objective: To evaluate the urinary nuclear matrix protein (NMP22) as an adjuvant diagnostic index for transitional cell carcinoma of urinary tract and monitoring the state of disease.

Methods: Urinary samples were collected from 262 patients with transitional cell carcinoma, 198 non-transitional cell carcinoma of the urinary tract and 65 patients with benign diseases. Urinary NMP22 concentration was determined through enzyme linked immunosorbent assay (ELISA).