Publications by authors named "Zhen-dong Zhou"
Targeted gene disruption by CRISPR/xCas9 system in Drosophila melanogaster.
Arch Insect Biochem Physiol
May 2020
Although the Cas9 protein from Streptococcus pyogenes (SpCas9) is the most widely used clustered regularly interspaced short palindromic repeats (CRISPR) variant in genome engineering experiments, it does have certain limitations. First, the stringent requirement for the protospacer adjacent motif (PAM) sequence limits the target DNA that can be manipulated using this method in insects. Second, its complementarity specifications are not very stringent, meaning that it can sometimes cause off-target effects at the target site.
View Article and Find Full Text PDFThe effect of borneol on the concentration of meropenem in rat brain and blood.
J Asian Nat Prod Res
September 2014
Meropenem is a carbapenem antibiotic with a wide spectrum of activity against both Gram-positive and Gram-negative bacteria. Because of its clinical efficacy, meropenem is an excellent choice for the treatment of serious infections in both adults and children. The knowledge of tissue concentrations of antibiotic in an infection site is valuable for the prediction of treatment outcome.
View Article and Find Full Text PDFObjective: Polyethylene glycol/bone morphogenetic protein-2 (PEG/BMP-2) nanoparticles were transfected into Rabbit bone mesenchymal stem cells (rBMSCs) and the expression of BMP-2 was detected.
Methods: Dissociated rBMSCs were primarily cultured in vitro and BMP-2 gene was transfected into rBMSCs by PEG/BMP-2 nanoparticals and lipofectamine, respectively. The efficiency of transfection was detected by flow cytometry and the expression of BMP-2 was detected by Western Blot and real time RT-PCR.
Article Synopsis
- The study examines the effectiveness of multiplanar reconstruction (MPR) from spiral CT scans in diagnosing and addressing sacral fractures associated with neurological damage.
- Conducted at Shengjing Hospital, it involved 10 patients aged 30-55, primarily due to accidents, with MPR helping visualize the sacral neural tube and the relationship between fractures and nerve injury.
- Results indicated that MPR identified significant nerve injuries related to sacral fractures, leading to tailored clinical diagnoses and treatments, ultimately aiding recovery for most patients.
Objective: To investigate the effects on HIF-1α expression of rabbit adipose-derived mesenchymal stem cells (ADSCs) by dynamic compression plus IGF-1 gene transfection and explore the mechanism of promoting chondrogenesis by HIF-1α.
Methods: The ADSCs were harvested after the digestion of typeIcollagenase and transfection with pcDNA3.1-IGF-1.