Publications by authors named "Zhen-Yuan Qi"
Establishment and assessment of two methods for quantitative detection of serum duck hepatitis B virus DNA.
World J Gastroenterol
September 2004
Aim: To establish and assess the methods for quantitative detection of serum duck hepatitis B virus (DHBV) DNA by quantitative membrane hybridization using DHBV DNA probe labeled directly with alkaline phosphatase and fluorescence quantitative PCR (qPCR).
Methods: Probes of DHBV DNA labeled directly with alkaline phosphatase and chemiluminescent substrate CDP-star were used in this assay. DHBV DNA was detected by autoradiography, and then scanned by DNA dot-blot.
Assessment of a method for detecting serum HBV DNA with HBV DNA probe labelled directly by alkaline phosphatase.
Hepatobiliary Pancreat Dis Int
November 2003
Objective: To assess a sensitive and specific technique for detecting serum HBV DNA with an HBV DNA probe labelled directly by alkaline phosphatase (AlkPhos Direc probe).
Methods: AlkPhos Direc probe was prepared with purified HBV DNA labelled directly by alkaline phosphatase. The probe and chemiluminescent substrate CDP-star for AP were used in hybridization assay.