[Study on absorption spectra of cell substrate for Hep-2 cell after being radiated by X-ray].

UV-absorption spectra of the Hep-2 cell's culture medium RPMI1640 (10% Foetal Calf Serum) were collected by UV-3101 spectrophotometer after the Hep-2 cell was radiated by X-ray and cultivated for 24, 48 and 72 h, and the absorbability of the proteins in the substrate was analyzed. From these results it was found that there were visible differences among these absorption spectra. In particular, the absorption peaks of the RPMI1640 culture medium during the cultivation shifted from 233 to 235 nm, while the absorption peak at 278 nm became more and more smooth and even finally disappeared with the cultivation time.