Publications by authors named "Zhen-Liang Qu"
[Investigation of hepatitis B virus integration sites in hilar cholangiocarcinoma tissues].
Zhonghua Wai Ke Za Zhi
August 2011
Objectives: To study the phenomena of hepatitis B virus (HBV) integration into the tissues of hilar cholangiocarcinoma (HCCA) and to identify the integration sites in the host genome.
Methods: Ten fresh HCCA samples were collected from the tissues by surgical ablation, 1 normal hilar bile duct sample selected as control. Cellular DNA were extracted by Wizard SV Genomic DNA Purification System.
Aim: To study the changes of human telomerase reverse transcriptase (hTERT) mRNA expression in human hepatocarcinoma cell lines (HepG2) and cholangiocarcinoma cell lines (QBC939) after HBx gene transfection and to illustrate the significance of transcriptional regulation of hTERT gene by HBx gene in the carcinogenesis.
Methods: HepG2 and QBC939 cell lines were cultured and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein (EGFP) coding sequence using lipid-mediated gene transduction technique. Thirty-six hours after transfection, EGFP expression in cells was used as the indicator of successful transfection.
[Culture and identification of human skin fibroblasts].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
February 2005
Objective: To explore the method of isolation, cultivation, and identification of human skin fibroblasts in vitro.
Methods: By digesting human skin with collagenase type II to isolate human eccrine sweat glands. The fibroblasts grew along with the growth of eccrine sweat gland cells,and they were separated by digesting with 0.
[Isolation and purification of eccrine sweat glands in human skin].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
February 2005
Article Synopsis
- This study aimed to isolate and purify epithelial cells from human eccrine sweat glands in a lab setting.
- Using collagenase type II, researchers successfully digested human skin to extract gland cells, with further purification achieved through multiple transfers under an inverted microscope.
- The results showed that collagenase type II effectively preserved the integrity of the cells while allowing them to grow in culture, overcoming challenges related to contamination from other cell types and tissue debris.
- Despite successful isolation and cultivation of sweat gland cells, the researchers acknowledged that there are still significant challenges in the process.
Objective: To study the effect of Hepatitis B virus X (HBx) gene transfection on expression of human telomerase reverse transcriptase (hTERT) mRNA in human bile duct carcinoma cell lines QBC939 and to elucidate the significance of cis-activation of hTERT mRNA by HBx gene on the carcinogenesis of bile duct.
Methods: QBC939 were cultured in vitro and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein (EGFP) coding sequence using liposome-mediated gene transduction technique. Thirty six hours after transfection, EGFP expression, the indicator of successful transfection in cells, was determined.
Aim: To study the transcriptional regulation of human telomerase reverse transcriptase (hTERT) mRNA in normal human cholangiocytes (HBECs) after hepatitis B virus X (HBx) gene transfection and to elucidate the possible mechanism of HBV infection underlying cholangiocarcinoma.
Methods: HBECs were cultured in vitro and co-transfected with a eukaryotic expression vector containing the HBx coding region and a cloning vector containing coding sequences of enhanced green fluorescent protein (EGFP) using lipid-mediated gene transfer. The transfection efficiency was determined by the expression of EGFP.
Objective: To detect the expression of human telomerase reverse transcriptase (hTERT) protein and mRNA in bile duct carcinomas and the adjacent tissues and to elucidate its role in bile duct carcinogenesis.
Methods: The expression of hTERT protein and hTERT mRNA in the formalin-fixed paraffin-embedded specimens of 71 cases of bile duct cancers and 39 cases of adjacent tissues was detected by streptavidin-peroxidase immunostaining and in situ hybridization. The correlation was analysed statistically between the expression of hTERT protein and mRNA and clinicopathological parameters bile duct carcinomas.
Objective: To detect the expression of HBV X gene (HBx mRNA) in extrahepatic biliary tract carcinomas and the adjacent non-cancerous tissues, and to analyzed the relationship between HBV infection and incidence of biliary tract carcinomas, thereby to elucidate the possible role of HBx in the carcinogenesis of biliary tract.
Methods: The plasmid pSPX46 was digested by appropriate restriction enzyme. HBx fragment was obtained through gel extraction kit.