Publications by authors named "Zhen-Hua Huang"

The accumulation of excessive nitrogen, phosphorus, and other nutrients in rivers, lakes, and reservoirs has greatly accelerated eutrophication, which has led to the frequent outbreaks of algal blooms and brought great ecological risks to the related aquatic ecosystems. Evaluations on the eutrophic status of water bodies and estimations of water environment capacity are not only crucial for comprehensive assessment of eutrophic status but also indispensable references for comprehensive management of the aquatic ecosystems. In this study, major environmental variables (chemical oxygen demand, total nitrogen, and total phosphorus) of Yankou Reservoir watershed were monitored monthly from May 2020 to March 2022 and based upon the determined results, the comprehensive eutrophic conditions and water environment capacity were evaluated and estimated.

View Article and Find Full Text PDF
Article Synopsis
  • Ex vivo lung perfusion (EVLP) is a technique that assesses and restores donor lungs, particularly beneficial for marginal lungs from donation after circulatory death (DCD).
  • The study examined the impact of milk fat globule epidermal growth factor 8 (MFG-E8) on lung function during EVLP using a swine model, revealing that MFG-E8 enhanced blood oxygen levels and reduced inflammation markers compared to other preservation methods.
  • The results indicate that using MFG-E8 during EVLP can improve lung graft performance, suggesting its potential for clinical application to expand the availability of DCD lungs for transplantation.
View Article and Find Full Text PDF

Background: Out-of-hospital cardiac arrest (OHCA) is a leading cause of death worldwide.

Aim: To explore factors influencing prehospital return of spontaneous circulation (P-ROSC) in patients with OHCA and develop a nomogram prediction model.

Methods: Clinical data of patients with OHCA in Shenzhen, China, from January 2012 to December 2019 were retrospectively analyzed.

View Article and Find Full Text PDF

The "bacteria-algae" system plays an important role in water ecosystems. The effects of bacteria in phycospheres on the growth of under in-situ nutrient stimulation were studied to explore the bacteria-algae interaction during a cyanobacteria bloom. The results showed that LB medium could inhibit the growth of , and the algicidal rate was 86.

View Article and Find Full Text PDF

The goal of this study was to investigate the homeobox (HOX) gene expression status and its prognostic value in glioblastoma multiforme (GBM) and to uncover the biological processes related to its expression. The prognostic value of HOX genes in GBM was systematically investigated by a genome-wide analysis of HOX gene expression profiles in GBM patient samples in The Cancer Genome Atlas (TCGA) project (microarray dataset) and validation datasets. Using the differentially expressed gene (DEG) analysis and a Cox regression model, we discovered that the HOXC6 could stratify patients into significantly different survival ( = 0.

View Article and Find Full Text PDF

The number of circulating endothelial progenitor cells (EPCs) was found to increase in patients with breast cancer, but the alteration in EPC function remains to be elusive. We conducted this study to evaluate the number and function of peripheral EPCs of breast cancer patients and its possible underlying mechanism. Besides, the vascular endothelial growth factor (VEGF), VCAM-1, IL-6, and IL-34 levels were measured in blood samples and also in vitro in a medium of EPCs.

View Article and Find Full Text PDF

Emerging clinical and experimental evidence demonstrates that neuroinflammation plays an important role in cognitive impairment associated with neuropathic pain. However, how peripheral nerve challenge induces remote inflammation in the brain remains largely unknown. The circulating leukocytes and plasma C-X-C motif chemokine 12 (CXCL12) and brain perivascular macrophages (PVMs) were analyzed by flow cytometry, Western blotting, ELISA, and immunostaining in spared nerve injury (SNI) mice.

View Article and Find Full Text PDF

Context/objective: Following acute spinal cord injury (ASCI) in rabbits, subarachnoid space injection of Danshen was performed to protect the neurological damage. In this study, we established rabbit models of spinal cord injury using a modified Allen's method.

Design: After the operation introducing the injuries, the rabbits were randomized into two different groups, control group (normal saline, NS) and Danshen, a component extracted from Chinese herb, treatment group.

View Article and Find Full Text PDF

Background: ZFAS1 is a newly identified long noncoding RNA (lncRNA) that promotes tumor growth and metastasis. Exosomes mediate cellular communications in cancer by transmitting active molecules. The presence of ZFAS1 in the circulating exosomes and the roles of exosomal ZFAS1 in gastric cancer (GC) remains unknown.

View Article and Find Full Text PDF

The accumulation of Cd2+ and Hg2+ in Phascolosoma esculenta and its effects on its growth and main nutrient components were investigated using atomic absorption spectrometer analysis and atomic fluorescence spectrometer examination. The results showed that within the experimental heavy metal concentrations, the amounts of Cd2+ and Hg2+ accumulated in the somatic muscles increased with increasing exposure time and reached their saturated levels at the end of the experiment. Exposure of higher heavy metal concentrations speeded up the accumulation of Cd2+ and Hg2+ and subsequently took less time to reach their saturated levels, which were greater than those of lower concentration groups.

View Article and Find Full Text PDF

Hyperacute rejection (HAR) is a main barrier in xenotransplantation, which is mediated by the combination of natural antibody to the xenograft and complement activation. Current therapies have focus on the inhibition of complement by development of complement inhibitor and transgenic animal organ. Here, we investigated the effects of rFII, a recombinant fibrinogenase from Agkistrodon acutus venom, on complement and HAR.

View Article and Find Full Text PDF

Background: Anxiety and fear frequently causes an aversion to applying a face mask and increases difficulty during pediatric induction. There is at present little study of this problem. Therefore, the aim of this study was to investigate the effect of the combination of mask preconditioning and midazolam pretreatment on mask acceptance during pediatric induction and on postoperative mask fear.

View Article and Find Full Text PDF

The incidence of disseminated intravascular coagulation (DIC), which leads to multiple organ dysfunction and high mortality, has remained constant in recent years. At present, treatments of DIC have focused on preventing cytokine induction, inhibiting coagulation processes and promoting fibrinolysis. Recent clinical trials have supported the use of antithrombin and activated protein C supplementation in DIC.

View Article and Find Full Text PDF

Histidine-hyaluronic acid (His-HA) conjugates were synthesized using hyaluronic acid (HA) as a hydrophilic segment and histidine (His) as hydrophobic segment by 1-ethyl-3(3-dimethylaminopropyl)carbodiimide (EDC) mediated coupling reactions. The structural characteristics of the His-HA conjugates were investigated using (1)H NMR. His-HA nanoparticles (HH-NPs) were prepared based on His-HA conjugates, and the characteristics of HH-NPs were investigated using dynamic light scattering, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and fluorescence spectroscopy.

View Article and Find Full Text PDF

Hydrophobic alginate derivative was prepared by modification of alginate by acid chloride reaction using oleoyl chloride without organic solvents. The conjugate of oleoyl alginate ester (OAE) was confirmed by FT-IR and (1)H NMR. The degree of substitution (DS) of OAE was determined by (1)H NMR, and it ranged from 0.

View Article and Find Full Text PDF

AIM:To construct the recombinant of HDV cDNA and HBV specific ribozyme gene by recombinant PCR in order to use HDV as a transporting vector carrying HBV-specific ribozyme into liver cells for inhibiting the replication of HBV.METHODS:We separately cloned the ribozyme (RZ) gene and recombinant DVRZ (comprising HDV cDNA and HBV-specific ribozyme gene) into the downstream of T7 promoter of pTAdv-T vector and studied the in vitro cleavage activity of their transcripts (rRZ, rDVRZ) on target RNA (rBVCF) from in vitro transcription of HBV C gene fragment(BVCF).RESULTS:Both the simple (rRZ) and the recombinant ribozyme rDVRZ could efficiently catalyze the cleavage of target RNA (rBVCF) under different temperatures (37°, 42° and 55°) and Mg(2+) concentrations (10mmol/L, 15mmol/L and 20mmol/L) and their catalytic activity tended to increase as the temperature was rising.

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessionbivva86opvcf91tn0ine3lcfg1od15bi): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once